Questions tagged [growth-media]

Nutrient solution for the growth of eukariotic cells or bacteria. It can contain everything the cell needs for its growth (complete media) or can be incomplete (selective media) to select for cells that are able to synthesize specific nutrients.

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Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
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17 views

Would BMP7 introduced to a Petri dish with cartilage cause chondrocytes to replicate?

Chondrocytes are the cells that make up cartilage. As I understand it, the best growth factor that signals replication of chondrocytes is Bone morphogenetic protein 7. If supplied with a sufficient ...
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804 views

Prevent clumping of bacteria in my media so that I can measure turbidity?

Forgive my naivety, I'm an undergraduate Chemical Engineering student doing research this summer into growth rates of various combinations of bacterial isolates. I have about 9 isolates from a toxic ...
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39 views

Can you grow more Biomass in a brickhouse only powered by solar cells than in a greenhouse? Will we ever be able to?

So I asked following question some physicists but they couldn't really help me though: Right now I read that 1m² of solar cells generates roughly 100Watts of energy. There are really efficient ...
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350 views

What is the practical difference between SC, SD and CSM?

Is there some logic to how SC, SD and CSM yeast dropout media differ from each other? I have encountered these three formulations in Sunrise Science Products' catalog, and it appears that the ...
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Is Cell Concentration a Major Causal Factor In Lag Phase Specific Growth Rate of Chlorella?

Literature on lag phase low specific growth rate seems invariably to assume a change in environment to which the single-cell organism must adapt before the specific growth rate reaches its maximum ...
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34 views

What’s this fungus growing in supersaturated salt solution

As a hobby, I’ve been trying to “grow” crystals of from supersaturated solutions of aluminium potassium sulphate. I noticed that my solutions are also rather successful at growing a type of fungus. ...
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19 views

Using a magnetic stirrer as a makeshift shaking incubator?

I'm working in my high school's lab and need to culture bacteria in a broth culture. More specifically, I'm trying to harvest Bacillus endospores with the exhaustion method published in "Molecular ...
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26 views

Scientifically testing when milk goes bad

How could I test if milk has gone bad scientifically? Performing a taste test on the milk yields a subjective interpretation as to whether the milk is good or not. Is there any objective metric that I ...
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Lecithin in Tryptone Azlectin Tween (TAT) media

When I made TAT in tank and separate in chamber, I found precipitation in some chamber, not at all. I asked my site manager and her tell me it's lecithin and normal. But, in my oppinion, I think ...