Questions tagged [histology]

Microanatomy of cells and tissues

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What renders a cytoplasm basophilic?

I know that being basophilic or acidophilic corresponds to affinity to certain dyes used in microscopy. What i want to know is what characteristics of the cytoplasm can we infer from its basophilic ...
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Modern views on the correspondence of moles

In his Memoirs, Casanova wrote: In the midst of the dimple which added such a charm to her chin Esther had a little dark mole, garnished with three or four extremely fine hairs. These moles, which ...
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Number of dopaminergic neurons in VTA

Do you know an authoritative source for the approximate number of dopaminergc cells in the ventral tegmental area (VTA)? Ideally I would like to know this for mice, rats, as well as humans, but one ...
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Quick overview of how to capture microscope images of histology plate or fluorescent plate

I am looking at these two: I would like to DIY take photographs of plant and animal cells using a cheap microscope such as this: I don't know too much about microscopes or the techniques to do the ...
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Distinguishing Dermis and Epidermis Layer

Hello, I have two histology slides of my skin equivalent samples that I received recently and was not sure if I was identifying my parts correctly. I just wanted to confirm that the most of my skin is ...
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Where is the cartilage plate in this electron microscope image of the trachea?

Also how could you tell? Assuming you were shown a photo of the cartilage only, how would you identify it?
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Histology cheek swab

I recently did a cheek swab and I can recognize the cells the nucleus and cell membrane, but I noticed a section that had a cluster of nuclei resembling dots. I was wondering if it was just cells ...
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Skin (histology)

Our instructor share this image with us. She didn't specify much. She only said it was a skin sample. Could you tell me what is the thing that was pointed at with the blue arrow? I believe that I ...
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What advantage does the pseudostratified epithelial structure type provide over squamous, cuboidal, and columnar?

I'm currently and have had difficulty receiving an answer about the role the pseudostratified columnar structure provides. I understand that that the following have their respective advantages: ...
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Do different microscopes and attached cameras differ in crop factor between each other?

I analyzed a bunch of microphotographs recently and used "% of image area" to describe the area of structures (chorionic villi, had to show their density in placenta). However my professor suggested I ...
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What is going on with these buccal epithelial cells?

A pooled saliva sample from a 70 year old male. What is happening with these cells? Are these cells undergoing normal apoptosis? In the video, the cytoplasmic movement looks liquefied and jelly-...
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FIbroblast vs fibrocyte [duplicate]

After i had been studing for histology examp i tried to solved one nominclature dilema.Its about fibrocytes and fibroblast. In my histology book is statment, that fibrocytes is special type of cell ...
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what is the exact definition of entdothelial cell fenestrae?

In fact, I want to know that are fenestrae 1.holes which exist between cells or 2.they are holes in a cell( for example, a cell is like a paper we have punched it and the holes are called fenestrae). ...
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What gives nerves their silver colour?

I always thought the silver colour specific to nerves was due to the myelin sheaths, but I've observed that unmyelinated C fibres display that same silvery appearance. Where does this colour come from?...
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What are null cells?

My histology textbook says the following: The pluripotential hemopoietic stemm cell (which resembles a lymphocyte) is a member of the null cell population of lymphocytes. It then goes on to add: ...
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Binucleate cells of transitional epithelium (bladder)?

Working through Histology slides of the transitional epithelium (urothelium) of the urinary bladder, I noticed that the dome-shaped cells at the top of the transitional epithelium are frequently ...
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What is the difference between the otolith membrane and the endolymph?

The human vestibular system contains otoliths to sense acceleration in the vertical and horizontal plane. I cannot find what the difference is between the otolith membrane and the endolymph? Can ...
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Why facet cells/umbrella cells of uroepithilium are sometimes multinucleated?

Why do are they multinucleated and why only some cells, why not all?
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How are neural pathways discovered?

I have seen the spinal cord and it's a white, soft, slippery cylinder that has a small diameter (about 25-30 milimeters). It absolutely doesn't have a macroscopocally reticular texture that would ...
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Is it correct to use the terms multinucleate and syncitia interchangeably?

If you see the skeletal muscle,it is having several nuclei and is said to be "multinucleate".Similarly the cardiac muscle is also having several nuclei yet it is specifically termed"syncitial".So my ...
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Why are the granules of pancreatic beta cells larger than those of alpha cells?

Why are the granules (insulin) of β cells larger than the granules (glucagon) in α cells in pancreatic islets of Langerhans? Also is there any size difference between alpha cells and beta cells? ...
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How to keep Paraffin Sections from falling apart?

I've been trying to make 5 uM sections of injured mouse spinal cords and stain the tissue with Luxol Fast Blue and H&E. At first I was removing the bones from around the spinal cord, but the ...
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Can formalin fixed tissue stored in absolute ethanol be used for histology?

Can samples which have been stored in buffered formalin and the moved straight to absolute ethanol be used for histology? I know that the samples normally need to go through a series of ethanol ...
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Gland defined based on morphology or hormone secretion?

Is a gland defined by a visible morphology, or is anything that secretes a hormone a gland? The answer does matter for the definition of histamine. Based on the answer I can conclude whether it's an ...
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What is this small tissue structure found in kidney?

It's covered with white adipose tissue and it is smooth with reddish brown colour. HE dyed with dimensions 3 cm long and 1 cm wide. I'm guessing that this is a rare thing because I already checked ...
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Histology tissue

Can anybody tell me from where is this tissue taken and in with techique is it colored please ?
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How can I download histological slide from Cancer Digital Slide Archive

Could you tell me if it possible and how to download images data from http://cancer.digitalslidearchive.net ? Thank you.
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What is the function of oxyphilic cells in the parathyroid?

Apart from the chief cells, which produce and secrete parathyroid hormone (PTH), the parathyroid gland has another type of cells, which are large and fewer in number, have small, dark nuclei and an ...
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Tissue identification in vascular bundle sections

I always have trouble differentiating the tissues in a vascular bundle. I specially cannot properly identify the vascular cambium and phloem. I know xylem vessel elements are large cells with thicker ...
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Why does spiral or annular thickening occur in water conducting plant vessels?

From what I behold, spiral and annular thickening of xylem and trachied cell walls leaves a lot of not thickened regions of cell wall. Lignin is the material which prevents water from escaping these ...
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Changes in spongy urethra during tumescence

How does the columnar cells of spongy urethra expand during tumescence(erection of penis)? If we assume that the urethra has the length, long enough to sustain tumescence without expansion of cells, ...
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Why are the walls of the bronchioles folded?

The bronchiole shown in the section above has folded epithelium, why is this so?
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Is the central axon of pseudounipolar neurons in dorsal root ganglia typically myelinated

As I understand, sensory nerve cells in the dorsal root ganglion are pseudounipolar neurons, with a sole protrusion from the soma branching into a central axon (or functional axon) and peripheral axon ...
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Why are there no Nissl bodies in the axon?

What could be the significance of not having Nissl bodies (endoplasmic reticulum) in the axon hillock (a part of the cyton) and axon? Does it have something to do with the processing of action ...
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DAPI staining showing unknown artefacts?

Dear fellow researchers, I have been facing some problem with staining nucleus with DAPI. I use the standard protocol for fixation 4%PFA+0.1% TX-100+2%BSA. I use 0.2ug/ml conc. of DAPI for 5 minutes....
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How does the ATPase stain for muscles revert on changing the pH?

Muscle sections are stained with ATPase to differentiate between types of fibres and to display the ckecerboard pattern. But it is done at two different pH. The staining is reversed at acidic/basic pH....
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What is “non-immune immunoglobulin”?

From a short introduction to immunohistochemistry controls: Isotype Control This control can be utilized when working with monoclonal primary antibodies. The sample is incubated with antibody ...
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Do our blood vessels exude chemicals?

I watched a youtube video explaining how mosquitoes detect our blood vessels through "chemicals that our blood vessels exude naturally" I was wondering if it's true that our blood vessels exude any ...
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Extraction of arthropod embedded in animal tissue

I'd like to extract an ectoparasite from a biopsy of animal skin for subsequent identification. The mite is small, about 100 um, and embedded in a column of tissue. It's too small to manually excise ...
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What is the meaning of the verb “lines” in this context? [closed]

What is the meaning of the verb "lines" in this context? "Epithelial tissue covers body surfaces, lines body cavities and form glands" I searched in the dictionary but I found a lot of results ...
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Heterochromatin v Euchromatin. Which is more abundant?

So I was just reading that whether heterochromatin or euchromatin is more abundant in a particular human cell depends on how active that cell is. But considering that most of the 25,000 or so genes in ...
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What are the exact differences between stereocilia and microvilli?

This is a question in an old exam of Histology. I tried to answer the question, and I've made many searching attempts, but without resulting in any fixed/exact information. In some articles on the web,...
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Can axons act as receptors?

In all histology books, it is stated that all sensory nerve endings (receptors) consist of dendrites that translate physical stimuli from the environment into neural signals. However, several sensory ...
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Why can't plasma proteins migrate from capillaries?

Why can't plasma proteins shift from capillaries to connective tissue but WBCs can be very rich in connective tissue even though obviously the WBCs had to go through capillaries. Another example: in ...
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What is the relationship between W, X, Y and P, M retinal ganglion cells?

In Guyton and Hall Textbook of Medical Physiology (12e) the retinal ganglion cells (RGCs) are classified into W, X, and Y types. However, in Gray's Anatomy (40th ed.), RGS are subdivided into midget ...
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Is this a legitimate teratoma for a human pluripotent stem cell assay?

I can't quite make out this teratoma:                            ...
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Why can we use mouse-produced antibodies on mice tissues?

I have seen biologists use mouse grown primary antibodies in mouse tissue, and they've told me that if the blood is perfused well then there is no problem with this method. How does the secondary ...
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Trying to differentiate between the three muscle tissues with small pictures

For an assignment I have to differentiate the three types of muscle tissues in these three pictures . I'm having difficulty seeing the striations and branches etc. because the pictures are so small. ...
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Why are Merkel cells innervated by an axon, and not a dendrite?

Here are two images from Google. Afferent neurons receive input and send it to the central nervous system. Input is received by the neuron's dendritic end and send away centrally via axon terminals ...
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Does methanol fixation deform cultured cells?

I use methanol fixation (@ -20⁰C for 10 minutes) when performing immunofluorescence assays on cultured cells. Generally speaking, this results in very good antibody staining. However, the cells tend ...