Questions tagged [histone-modifications]

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21 votes
1 answer
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Can siRNA induce DNA methylation in mammalian cells?

Some years ago Hiroaki Kawasaki and Kazunari Taira published an article called "Induction of DNA methylation and gene silencing by short interfering RNAs in human cells" in Nature: In plants, ...
Mad Scientist's user avatar
15 votes
1 answer
2k views

How are epigenetic marks transmitted during cell division?

As far as I know, this is one of the biggest questions in the epigenetic field: how are the epigenetic marks like histone modifications propagated through cell division? A lot is already known about ...
Gergana Vandova's user avatar
6 votes
1 answer
337 views

How does "inheritance of methylation" of DNA and/or histones work?

What are the current models/ideas describing the mechanisms explaining inheritance of methylation on DNA resp. histone level? Is there evidance of this "setup" information being really duplicated/...
pointernil's user avatar
2 votes
2 answers
135 views

ChIP-seq for histone modification not in agreement with RNA-seq for expression

I have ChIP-seq for H3K79me2 and H3K36me3 and RNA-seq data for treated and untreated samples. Those two histones mark active genes. Lets say, hypothetically, a peak caller finds differential sites at ...
Alina's user avatar
  • 363
2 votes
1 answer
392 views

H3K36me3 role in RNA polymerase II elongation

I found a paper describing the association of RNA polymerase II with the histone methyltransferase Set2 that methylates H3K36. At the end they say: While our results strongly implicate K36 ...
Alina's user avatar
  • 363
2 votes
2 answers
422 views

How does mass spectrometry detect histone methylation?

In this research paper they talk about the methylation of histone. They use mass spectrometry to identify which peptide position is methylated. Does the number yielded by the spectrometer calculate ...
Scsqpd's user avatar
  • 195
2 votes
1 answer
51 views

What signals histone methyl transferases to function?

I wanted to find out if anyone knows what stimulates histone methyltransferases to function? I know that it is a process still trying to be understood, but I can't find anything that seems to direct ...
Carina's user avatar
  • 21
2 votes
2 answers
71 views

Can one talk about deacetylation of a promoter rather than associated histone?

I am confused on a detail in a paper I am reading and am not sure whether I am misunderstanding the wording or misunderstanding the concept. I am including the whole abstract of this paper for ...
mshum's user avatar
  • 147
1 vote
0 answers
81 views

What determines the differences between differentiated cells?

Given two multi-cellular species with obviously different phenotypes. The reason for the different phenotypes reflects their different DNA. However two types of cells in an adult organism may have ...
Hans-Peter Stricker's user avatar