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Questions tagged [introns]

Regions of a gene which do not form a part of the mature RNA product.

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How do introns code for proteins? [duplicate]

The reason I am asking this is because my textbook states that: Some introns may themselves encode proteins, and some may become short non-coding lengths of RNA involved in gene regulation. Some ...
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1answer
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Do the genes encoded by the nucleus for mitochondrial proteins have introns?

Mitochondria are believed to have transferred much of their genome to the nucleus. I know that mitochondrial protein-coding genes lack introns, but is this true for mitochondrial genes encoded by the ...
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Why aren't 'exons' named 'introns'?

Why are introns called 'introns' when they are the actual ones who are getting spliced out from the pre-mRNA. Shouldn't exons be named introns as they are the ones that are 'in' and are not 'exiting'? ...
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Can you splice out the first and last exon from pre-mRNA?

I think the answer is no because there are no introns before the first exon or after the last exon, although I am not sure if this assumption is correct. Thanks!
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Clarification on RefSeq Genes track on IGV

The following picture is a snapshot of the RefSeq Genes track (collapsed) on IGV. I know that the bold blue line indicates exons and the thin one indicates introns. However, I cannot find what the ...
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What happens to the cut-out introns from a pre-mRNA?

Once the spliceosome has cut out the introns from a pre-mRNA they are said to be degraded. What does that mean and is that happening in the cytosol? Can the nucleotides be used for something else ...
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132 views

Confirming exon shuffling in a gene

I'm trying to confirm that the sequence of a novel gene is derived by exon shuffling between several different genes. I have the promoter sequence, gene sequence, and mRNA (with defined exon/intro ...
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1answer
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Excluding the Exon, What Does the Rest of the Genome Do?

The exon is the portion of the genome that encodes protein synthesis, if I understand correctly. But, this exon is apparently less than 5% of the genome. So, what does the rest of the genome do?
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Why is mutation rate high in introns?

Principle of genetics mentions The fact that introns accumulate new mutations much more rapidly than exons indicates that many of the specific nucleotide-pair sequences of introns,excluding the ...
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Why are minor introns bounded by nucleotides AT-AC, where T is not normally in RNA?

Major intron boundary nucleotides are GU-AG. Minor intron boundary nucleotides are AT-AC. Why does the former use RNA Uracil and the latter use DNA thymine? Since introns are in the primary RNA ...
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1answer
850 views

How does the splicing enzyme recognize where to splice the introns?

When the DNA from the nucleus is transcribed to an mRNA, the mRNA is spliced by an enzyme before it goes outside through the nuclear pore. What is the name of this enzyme and how does it recognize ...
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What are the reasons which make intron detection uncertain?

Intron/exon sequence detection seems to involve statistical prediction which can at best deliver a guess (until experimentally confirmed) as to where the splice site is. What are the reasons why ...
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Is intron removal coordinated?

Are the introns present in a nascent rna molecule removed simultaneously or are they removed in some sort of sequential fashion or is removal completely random?
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Transcription factor binding site located in intron

I have noticed that some TF binding sites are located in the introns of the genes. I am puzzled about whether the TF only binds to DNA in the initiation stage of transcription and will detach during ...
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2answers
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Detecting Introns and Exons

I know that when RNA is transcribed from the original strand of DNA it contains introns and exons, and that the introns are spliced out of the strand to provide genetic diversity. However, what I don'...
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How to find Exons in mRNA Computationally

I'm having trouble finding a method to find exons in the original DNA sequence used to create the mRNA, even given the sequence of the mRNA, as I cannot find a way to reliably identify the beginning ...
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Why do eukaryotic organisms have introns in their DNA?

We touched on introns and exons in my bio class, but unfortunately we didn't really talk about why Eukaryotes have introns. It would seem they would have to have some purpose since prokaryotes do not ...
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Could Junk DNA be used as a Turing Machine by nature?

In what ways has DNA been studied to see if there a "programmable" aspect to it? Has nature produced anything resembling a Turing machine within the cell, perhaps using the "junk DNA" as its code? I ...
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What are limiting factors for intron length?

For predicting genes from a sequenced genome, you need to set a maximum intron length. How long can introns get in animals? Is there some limit?
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Is exon order always preserved in splicing?

Are there any cases in which the splicing machinery constructs an mRNA in which the exons are not in the 5' -> 3' genomic order? I'm interested any such cases, whether they involve constitutive or ...