Questions tagged [lentivirus]

A genus of retroviruses with a lengthy incubation time, in particular HIV. Commonly used as DNA transduction agents in animal cells.

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Should gene therapy safety protocol include isolation?

In the case of a gene therapy trial where viral vectors are used to deliver genes into mammalian cells, including humans, should biosafety and ethical protocols include isolation of the patient as a ...
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Virus titre and copy number of integration

If I need to preform a genome-wide high throughput screen using pooled shRNA library infection followed by deconvolution by deep sequencing, how would I determine the optimal infection rate at which ...
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Lentiviral vaccine vector

If you have to make a vaccine, where the non-integrating lentivirus is used as vector. Would you then insert the surface proteins (the antigens targeted in the vaccine) in the Env-plasmid or in the ...