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Questions tagged [ligation]

Is the process of covalently link two DNA ends. The process is usually done by the enzyme class of ligases.

18
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2answers
368k views

What does 5' and 3' mean in DNA and RNA strands?

What are 5' and 3' in DNA and RNA strands? Please clarify with some images and please use simple English.
5
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2answers
878 views

What are common causes of unexpected ligation products?

I digested two plasmids, one with EcoRI and AgeI and the other with EcoRI and XmaI. Digests looked as expected, so I purified the respective fragments and set up the T4 DNA ligation (AgeI and XmaI ...
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vote
1answer
2k views

circularizing DNA molecules?

I have been reading about next-generation sequencing technologies that can sequence long reads. Even though the origin of my question is sequencing technologies, the question I am asking is about the ...