Questions tagged [mammalian-cell-culture]

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Is this contamination, debris?

I have been culturing SVG cell lines. This line was obtained as a live culture from another institute and was delivered by mail. After subculturing , I saw some particles, and I do not know what they ...
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What is the difference between an immortalized human cell line and a cancerous cell line?

Many cell lines (like CaCo2) are derived from cancerous tissue, and hence, like any other tumor cell, they have an infinite replicative potential. However, many cell lines like HaCaT cells are not ...
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Single-cell subcloning of BV-2 cells?

I am trying to knock-out genes in BV-2 cell line. However, the majority of protocols require cell subcloning and expanding. I tried to subcone these cells and grow clones in DMEM+10% heat-inactivated ...
Monika1223's user avatar