Questions tagged [mass-spectrometry]

The tag has no usage guidance.

3 questions with no upvoted or accepted answers
Filter by
Sorted by
Tagged with
2
votes
0answers
32 views

Do “real” human samples tend to return fewer protein identifications in Proteomics experiments?

I'm working with bottom-up (shotgun) mass spectrometry-based proteomics and have encountered an issue. If I do a run with a HeLa digest standard, the resulting data returns many protein IDs. When I do ...
2
votes
0answers
533 views

Why do my peptide samples turn yellow and red?

The protocols for (brain) tissue digestion I am trying out are: lysis (buffer containing 0.1% SDC or SDS in 100mM TEAB + phosphatase inhibitor which is sort of yellow), reduction with TCEP, alkylation ...
1
vote
0answers
43 views

Why is carbonylation added as a fixed modification in mass spectrometry peptide analysis?

When using mass spectrometry analysis software we use carbonylation as a fixed modification. What is the significance of this modification and why doesn't it change?