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Questions tagged [medium]

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1
vote
0answers
85 views

Yeast transformation plate confluent with growth

I have problem with yeast transformation. I tried to transform pyes3-tryp(my insert inside) into INVsc1 yeast cell by following the protocol in the manual for few times but failed(confluent with ...
4
votes
1answer
60 views

Medium for Pseudomonas?

What is a good minimal medium for Pseudomonas bacteria? Do I need different media for different Pseudomonas species?
3
votes
1answer
117 views

Can a plate with incompletely filled LB agar be used?

I am falling short of LB agar plates and I have a plate with less LB agar poured. I meant, the plate is not fully filled with the agar (it was the last plate I poured the medium into). Some part of ...
0
votes
1answer
898 views

How the concentration of an antibiotic is chosen for YPD medium?

I need to prepare YPD plates containing hygromycin, but I don't know which concentration I can use. I'll select transformants of Hansenula polymorpha.
8
votes
2answers
102 views

Can paper/plastic currency serve as a medium for pathogens?

http://www.nytimes.com/2009/12/15/health/15real.html seems to indicate for a surface, to serve as a medium, the following properties are relevant animation humidity temperature The article ...