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Yeast transformation plate confluent with growth

I have problem with yeast transformation. I tried to transform pyes3-tryp(my insert inside) into INVsc1 yeast cell by following the protocol in the manual for few times but failed(confluent with ...
att901's user avatar
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How the concentration of an antibiotic is chosen for YPD medium?

I need to prepare YPD plates containing hygromycin, but I don't know which concentration I can use. I'll select transformants of Hansenula polymorpha.
Armacino's user avatar