All Questions
Tagged with methods microbiology
7 questions
1
vote
2
answers
191
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Storing bacteria in a dry, room temperature long-term storage whilst maintaining viability
I'm quite new to biology, but I'm wondering if there are any methods of storing bacteria in long-term, dry conditions with no climate control. Perhaps they could be dormant in a powder or granule-like ...
- 4,832
5
votes
0
answers
66
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Methods for getting clean C. difficile spore preps without spores clumping together
I'm a microbiologist recently who recently started working on an ongoing C. difficile project for the first time. One of the things I need to do is verify the lower detection limit of my qPCR ...
- 2,941
0
votes
0
answers
22
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How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?
I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
- 715
4
votes
1
answer
1k
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Using catalase-test to determine if Staphylo- or Streptococcus
If the microscope clearly shows bacteria of cocci morphology but it is difficult to determine if they are Staphylo- or Streptococcus, are catalase tests a good way to differentiate between the two ...
- 17.9k
0
votes
2
answers
1k
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Biology experiment with algae method help
I'm planning a biology experiment I need to complete. I was planning on measuring the affect of pH on algae growth. I chose chlorella to be the test algae I use (are there any algae that would be ...
- 17.9k
1
vote
1
answer
417
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Positive control for microorganisms
I'm planning to test the antimicrobial activity of endophytic fungi (their secondary metabolites) against Gram-positive bacteria, Gram-negative bacteria, and one fungus (Candida).
I was wondering ...
- 8,149
1
vote
1
answer
478
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Well Plate Method VS Kirby Bauer Method
Any pros and cons? One puts the test substance in sterile discs, while the other one puts it directly into wholes in the agar. Any thoughts?
- 1,529