Questions tagged [methods]

The methods tag can be used for questions on experimental hardware; experimental set up; experimental procedures; surgical techniques etc.

21 questions with no upvoted or accepted answers
Filter by
Sorted by
Tagged with
4
votes
0answers
27 views

How do scientists determine the nature of ions passing through a channel/carrier/pump?

The NCE (Sodium Calcium Exchanger) transports 3 Na+ inside the cell for 1 Ca2+ outside. How did we figure this out, and other mechanisms of this sort? If it were a protein, we could tag it with GFP. ...
4
votes
0answers
51 views

How to anaesthetise mosquitoes during a mass-trapping field study?

Do any of you have experience in anaesthetising mosquitoes during a mass-trapping field study? Normally, mosquito traps have a little net in which you catch the mosquitoes. The nets can, after ...
3
votes
0answers
290 views

Is there a standard formula/calculation for sexual dimorphism?

Is there a standard, or most accepted, way to present a value for sexual dimorphism? For example, one could use $$\frac{\text{Female value}}{\text{Male value}} -1$$ This would give negative values ...
3
votes
1answer
218 views

What is an extinction test?

In Stolerman & Olufsen (2001) I read the sentence: After drug-appropriate responding with the training mixtures reached 85%, generalisation to ethanol was examined in extinction tests. Here ...
2
votes
0answers
35 views

Nucleic acid extractions

Is it fine to leave pulverized plant tissue in CTAB buffer at -80 degrees over night? will the buffer remain effective to generate good quality nucleic acids?
2
votes
0answers
68 views

PCR limits of detection calculate method

Is any standard method for use to gain PCR sensivity or limit of detection? my mean is not for RT-PCR or Real-Time or any other kind and just simple PCR. After making PCR product free of dNTP and ...
2
votes
0answers
39 views

Stable isotope sample preparation: Bone Collagen

I am performing research in which I need to extract bone collagen from the foot bones of American Beaver (Castor canadensis). I have read through the literature fairly thoroughly on both the ...
1
vote
0answers
13 views

Does the preservation fluid (ethanol or formaldehyde) leach heavy metals from mollusc tissue?

In a thread on researchgate, the concern was raised that long term exposure to unbuffered preservation fluids such as ethanol or formaldehyde may leach heavy metals from biological samples due to a ...
1
vote
0answers
52 views

What kind of sieve does one use to extract meiofauna?

Suppose I have a core of sediment, and I want to extract the meiofauna from it, in order to study these organisms. According to the sources I could find, this involves using a very fine (e.g. 45 μm) ...
1
vote
0answers
17 views

Determine planar phyllotaxis characteristics

Is there any method to fast and precise count the number of spiral and the divergence angle on a planar phyllotaxis pattern? The example of spiral phyllotactic arrangement of emerging flowers on ...
1
vote
0answers
59 views

Protein expression in K. lactis system

I am trying to express a protein in K. lactis Protein Expression Kit (E1000S - NEB) in the way that the protein is secreted into the medium. I know that I can get some protein but it is not active. As ...
1
vote
0answers
38 views

Where can I get fungal extracts?

Does anyone know how I can get fungal extracts from fungal isolates? I have isolates in PDA slants and I'm gonna need to get their respective extracts to impregnate in sterile discs. I'm a novice in ...
1
vote
0answers
47 views

Spectroscopy on plant leafs

I'm trying to do spectroscopy readings on plants where I shoot electromagnetic waves and look at their signature/profile. I want to be able to do this at a distance, in the field and on live plants ...
1
vote
0answers
141 views

What is/are the effect(s) of altering the Tris concentration on polyacrylamide gels?

Why do we use two different concentrations of Tris (i.e. 1.5M Tris in resolving gel and 1.0M Tris in stacking gel)? For the gel to run properly there needs to be a different pH, but that can be done ...
1
vote
0answers
893 views

How to calculate the LOB, LOD and LOQ of an enzyme assay

I understand how to calculate limit of blank (LOB), limit of detection (LOD), and limit of quantitation (LOQ) in the traditional way i.e., average and SD of raw analytical signal of blanks and low ...
1
vote
0answers
62 views

Walkthrough Illumina Genotyping

I would love help outlining a basic walkthrough of the wet-lab techniques for processing blood samples from patients, all the way to loading a BeadChip, to the part just before Next Generation ...
0
votes
0answers
12 views

Insect collection question: Where to find anchor pins for specimen vials

Where can I purchase anchor pins for insect specimen vials for an insect collection. They look like they are in an 'M' shape,sort of, and stainless? will hopefully hold better than staight pins.
0
votes
0answers
22 views

Why don't materials and methods describe culture media in volume?

After reading several materials and methods sections of cell culture experiments, I have not seen the recipe for culture media ever described in terms of volume. Human adipose tissue was obtained ...
0
votes
0answers
19 views

How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?

I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
0
votes
0answers
46 views

How to find papers about voltage imaging (with GEVI) for human brain research?

I would like to follow the Nature article https://www.nature.com/articles/d41586-018-06694-6 about voltage imaging of brains, about use of GEVI - Genetically Encoded Voltage Indicators. But I am ...
0
votes
0answers
20 views

How can I approach this data that has a different total number of results between samples?

I was not sure whether I should post this in Biology Stack Exchange or Math Stack Exchange so I posted it in both. I was planning on doing a small research for my math class (a final project for the ...