Questions tagged [methods]

The methods tag can be used for questions on experimental hardware; experimental set up; experimental procedures; surgical techniques etc.

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1answer
1k views

What are wave frequencies in the EEG?

Here is a typical EEG reading: (source: frontalcortex.com) I understand that each row corresponds to the signal read between two sensors on a standard 10-20 (or 10-5) distribution setup (e.g. <...
2
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1answer
137 views

Efficacy of the 10-20 montage for EEG

I am trying to better understand the relationship between the placement of EEG electrodes and the quality of the "brainwave" signals they produce. There is the standard 10-20 system which has me ...
2
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1answer
2k views

What are the highest and lowest amplitude values in ECG for humans?

I'm currently trying to parse the records captured from an ECG device and display them on a mobile application. I am wondering what the highest and lowest possible amplitude values for ECGs are in ...
1
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1answer
50 views

How to incorporate intraspecific variation in discrete characters in comparative analyzes?

I'd like to run a multivariate analysis (Pavoine et al. 2011) that aims to disentagle the effect of environmental gradients on traits, considering its autocorrelation with phylogeny and space. It uses ...
1
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0answers
893 views

How to calculate the LOB, LOD and LOQ of an enzyme assay

I understand how to calculate limit of blank (LOB), limit of detection (LOD), and limit of quantitation (LOQ) in the traditional way i.e., average and SD of raw analytical signal of blanks and low ...
3
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1answer
93 views

How to best conserve a beetle inside a jar?

Few days ago a giant beetle appeared on my house. Well, it's dead now. Since it attracted some attention from my little girl, I'd like to preserve it inside some jar. What's the best way to do it? ...
1
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1answer
67 views

What are known methods of non-surgically disabling neural tracts/pathways (esp. Corpus Callosum)?

I think I've read something about usage of TTX and optogenetics, but I cannot find the papers for either anymore. Any reference would be much appreciated, especially those concerning mice and the ...
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0answers
62 views

Walkthrough Illumina Genotyping

I would love help outlining a basic walkthrough of the wet-lab techniques for processing blood samples from patients, all the way to loading a BeadChip, to the part just before Next Generation ...
2
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1answer
180 views

Genomic DNA isolation from wheat

Can I use dry seed, wheat for example, in place of young leaves for isolation and purification of genomic DNA for PCR amplification? The goal of my experiment is to validate a novel gene which is ...
0
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1answer
99 views

How can I fit the A595 of my unknown samples to the data?

While I was conducting the Bradford Assay experiment to determine the concentration of my unknowns, I ended up by having an A595 for my unknown samples that is higher than the A595 of the standards. ...
1
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2answers
33k views

What is the effect of the wavelength on the spectrophotometric measurements of protein?

Does the wavelength setting affect the absorbance reading of a spectrophotometer? For example, if I am using a spectrophotometer at 550 nm to determine the protein concentration of my samples, would ...
5
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1answer
77 views

How are the physiological properties of mitochondria measured?

this is my first question on BiologySE. I am a Physics and Mathematics student currently doing a project on cell growth simulation. I am doing literature survey and I have a question about cellular ...
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3answers
1k views

LCMS/MS versus Western Blot

I have a general question regarding which method would you recommend me to use if I would like to investigate the difference in the level of several proteins in tissue samples and compare different ...
2
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1answer
131 views

How is magnet based bead capture used for indexing-first ChIP-seq?

The context for this question can be found in this paper by Lara-Astiaso et al. Here, to study H3K4 trimethylation in various cell types, they sort cells sonicate chromatin immobilize chromatin on ...
4
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1answer
1k views

Measuring body size in flies/insects

"Mesothorax length (the distance from the tip of the scutellum to the most anterior part of the mesothorax)" From Bergland et al 2008. Would that be this distance (green line)? If not, how would ...
15
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1answer
214 views

Doing a medical study on yourself?

What research guidelines is a person legally mandated to follow if they want to do medical experimentation on themselves? There is a large amount of variation in the types of medical experiments so I ...
6
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1answer
322 views

What are the units of Phylogenetic Independent Contrasts (PICs)

PICs are a popular phylogenetic comparative method and I was wondering what units the actual contrasts are in. I want to say they are unitless but I am not entirely sure.
4
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1answer
174 views

How can I reduce the background gradient in phase contrast microscopy?

I'm looking for ways to set up a phase contrast microscope to reduce the brightness gradient that appears across the background of my images. I use phase contrast to find the outlines of cells in my ...
3
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0answers
291 views

Is there a standard formula/calculation for sexual dimorphism?

Is there a standard, or most accepted, way to present a value for sexual dimorphism? For example, one could use $$\frac{\text{Female value}}{\text{Male value}} -1$$ This would give negative values ...
3
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1answer
114 views

NMR spectroscopy detection methods for proteins

I've stumbled upon a table comparing the sensitivities of different detection methods in NMR spectroscopy: "Direct", "DEPT" and "Inverse", where "Inverse" seems to be the most sensitive. What exactly ...