Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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Why do bacteriophages look like tiny little robots? [closed]

I get it, evolution is funny and works in mysterious ways. But come on. You can't tell me this thing doesn't look like a nanobot. Why do bacteriophages look this way? With their cylindrical heads and ...
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65 views

How to get crisp images on the microscope?

I am using Nikon TS100 (Microscope) to take images of fluorescent green and red dye to see live and dead cells of Shewanella MR1. Visually on the microscope, the images look fine. However, when I ...
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Does anyone know of any other papers investigating this polymer mechanism idea?

https://www.google.com/amp/s/amp.dw.com/en/german-researchers-are-working-on-a-tissue-that-will-tell-you-whether-youve-got-the-flu/a-17030239 I am having trouble finding any other papers that describe ...
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If brine salt percentage is between 1 and 2 percent, can Clostridium botulinum growth prevail over Lactobacillus acidophilus?

After searching in various websites, I found mostly that Clostridium botulinum doesn't grow easily in a salty environment, and that it needs a pH from 4.6 to up; as I am lactic fermenting some bell ...
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Doubts about what is growing in my bacteriological cultivation

Foreword: I don't belong to the field at all, I am a high school student with a passion for science so I apologise in advance if I formulate the question incorrectly or make some mistakes. Out of ...
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Do bacteria produce proteins other than ribosomal proteins when grown in wrong culture media?

Someone I know has done an experiment using pure culture and given it to me for rna-seq analysis. But there are a few problems the bacteria she gave me as reference was initially a Staphylococcus sp. ...
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623 views

Limitations of 16S rRNA sequencing

Looking at the microbiome analysis literature there seems to have been a shift away from 16S rRNA sequencing analysis toward shotgun sequencing of the whole genome. While the motivation given is ...
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22 views

Do microorganisms produce more glucose in their extracellular environment than they need? Why?

Trichoderma reesei breaks down cellulose into beta and alpha glucose by secreting cellulases. Why do they produce far more glucose then they need when incubated with susbtrate?
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133 views

Do viruses do anything other than multiply? [closed]

Viruses are "on the edge of life ". They infect the cells and use it for multiplication of themselves but other than that do they do anything else like "eat food" like an amoeba ...
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25 views

Why crystal violet inhibits growth of gram positive bacteria?

In microbiology lab, we use MacConkey agar to selectively grow Gram-Negative bacilli. Crystal violet is used as a component of MacConkey agar for inhibiting growth of Gram Positive bacteria. However I ...
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How long for a ring of mold to grow in bathtub?

I stayed in an Airbnb for the past month, and the landlord is blaming me for mold around the shower drain, which was caused by a leaky shower. However, I remember the mold ring being there when I ...
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80 views

Is there any alternative to peptone and beef or yeast extract?

I am trying to start a small microbiology project and I need a growth culture for bacteria. I do not want to use the costly nutrient agars you can buy online. I would like to create my own nutrient ...
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1answer
56 views

Can a polymer coated with antibodies have enough attraction to wrap around a virus?

Archived source for image Please ignore the signal release. Does anyone know if it’s possible for polymer to wrap around a virus?
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Does a rapid cooling step improve pasteurization of medical equipment?

I learned that pasteurization consists of two steps: first heating and then rapid cooling. The rapid cooling was explained as "it's a thermic shock for the germs, so the ones remaining after the ...
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22 views

How do I cover Cellstrainers?

I‘m doing some experiments for my bachelors thesis and I‘m using some Cell strainers by Roth for it (https://www.carlroth.com/com/en/accessories/cell-strainer-easystrainer™/p/cly9.1). My problem is ...
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1answer
121 views

Is there any chance that the COVID-19 virus could become more deadly by it interacting with the HIV virus?

I would like to know if there is any chance that the COVID-19 virus, or one of its variants, could become more deadly by interacting with the HIV virus. Let's say for example, that a person who has ...
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1answer
38 views

Why log pathogen reduction for pasteurization validation and not absolute counts?

I notice that most guidelines for heat treatment processes such as pasteurization or sterilization use a "x-log pathogen reduction" target for validating the success of such processes. e.g. ...
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14 views

Microorganism ID?

I found this microorganism in a wet moss sample. It was very active and moved around quite quickly, and it looked to be using cilia to move. It had no coloration. I got some pictures at 400x: Any ...
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1answer
29 views

Disposing biohazardous material

I had conducted some disc diffusion tests with E.coli and S.epidermidis. Now, I wanna dispose these petri dishes and was wondering the correct way to do it. Many websites mention adding bleach to the ...
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1answer
28 views

ID microbe found in moss sample (USA)

I found this microbe after putting a wet moss sample under my microscope. It latched on to a piece of moss and scanned the area around it for prey, and when it didn't find any, it slowly swam away. It'...
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1answer
75 views

Why does open olive oil keep so long?

This is a question about the biology of microorganisms, not about food safety. While most of our foods spoil quite quickly (it is recommended to throw food away after 2h in the danger zone), for some ...
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32 views

Methods for getting clean C. difficile spore preps without spores clumping together

I'm a microbiologist recently who recently started working on an ongoing C. difficile project for the first time. One of the things I need to do is verify the lower detection limit of my qPCR ...
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Does Tryptic Soy Agar (TSA) contain L-cysteine? (Question about Legionella)

I ask because Legionella sp. requires the presence of cysteine. If TSA does contain L-cysteine, is it sufficient to grow Legionella?
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Do E. coli change color (from white/grey to yellow) when they grow older?

In my experiment, I had white colonies initially and they turned yellow. So I thought it´s natural that mature E. coli colonies are yellow and young are white. But it could be that I had two types of ...
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1answer
63 views

Isolating colonies in a pure culture

I want to conduct antimicrobial susceptibility tests for which I've to culture bacteria first. Since I've bought KWIK-STIKs, (KWIK-STIK contains a single lyophilized microorganism which have to be ...
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1answer
48 views

Does SARS-COV-2 produce toxins?

I wonder whether toxins are produced only by bacteria and fungi and not by viruses. Looking at the paper below, I read that several toxic proteins are produced by SARS-COV-2. Can these proteins be ...
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1answer
73 views

How to convert system of equations with explicit substrate consumption into an implicit one?

I have a description of competition between two bacteria that is described by the system of equations: S = substrate, μ = bacterial division rate, K = half saturation constant, y = yield (cells per ...
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1answer
40 views

What is targeted gene disruption?

I am a bit confused about what targeted gene disruption means. I was reading this article in which they compare the Pyrococcus Furiosus genome with a genetically tractable strain P. Furiosus variant ...
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How do fungal hyphae dig into substrates (such as grains)?

When a fungus needs to expand its nutrient-acquiring network of hyphae, it can dig into, say, a grain of rice. How does it drill openings into the grain?
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51 views

How do scientists create an environment that favors the selection of a microbe with higher production of a certain enzyme?

Let's say you want to create a strain of bacteria that is resistant to heat. You expose all bacteria to high heat and those who have mutated to survive this heat will be the survivors. Then you allow ...
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1answer
503 views

This graph about logarithmic and arithmetic plots. Shoudn't it be the other way around?

From the textbook 'Brock biology of microorganisms, 15th edition' The black lines points to the linear graph with the caption 'Logarithmic' and to the curved graph with the caption 'Arithmetic'. Isn't ...
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1answer
113 views

What is the relationship between a "main" strain genome and its variant genome in archaea?

I am going to analyze DNA sequencing data in order to try to extrapolate some information about the survival strategy of Pyrococcus Furiosus to gamma irradiation, as maybe you already know from all ...
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What is the purpose of putting multiple blanks on a microplate reader? [closed]

I am new to biology and I saw that in the software that controls the microplate reader several targets are set, what is the purpose of this?
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How can I learn botany/plant ID by myself?

I am interested in sources and methods regarding botany and plant ID, and being a self-taught person. Textbooks, sites etc. Thank you all in advance!
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How to express total eradication of bacteria (inhibition zone) when placed into a table

I am a grade 9 student studying the inhibition zones of several different essential oils by comparison to Tetracycline to prove there is merit to treatment of bacterial infection (investigation is ...
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1answer
112 views

Does one wing of fly contain harmful microbes and the other contain only antibodies?

There was this study but the conclusion and it's source's relability seemed a bit off. How come a house flies one wing contain all microbe and the other don't.
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In what stage of bacterial growth can penicillin inhibit the growth of the cellular wall?

The bacterial growth has 3 main stages: lag-phase, log-phase and stationary phase. I was wondering in which one of them penicillin can inhibit the growth of the cellular wall of bacteria and why. I ...
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Can the metatranscriptomics replace the approach of functional metatranscriptomics/functional metagenomics?

While metatranscriptomics reveals information about the expression of genes and their functions too, Functional metatranscriptomics (https://www.nature.com/articles/ismej201167) allows the ...
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1answer
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Is the stuffy environment due to using masks all day, helping in spread corona virus in people?

I came across a research that stated, the most disease ridden place in a toilet is actually the air dryer. The hot and moist microcosm that develops around the dryer due to frequent use helps in ...
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51 views

Are there any organisms that perform photosynthesis-like processes with thermal energy alone?

The closest I can find are thermophiles, but it seems they use the chemicals present in the hot environment for energy, not the heat itself.
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Nutrient agar as the medium for antibiotic susceptibility testing

I couldn't get Mueller Hinton agar to conduct antibiotic susceptibility testing. I'm thinking of just choosing nutrient agar as the growth medium for the bacteria Staphylococcus epidermidis. Will ...
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1answer
51 views

What does T and the numbers mean in T coliphages?

I have searched a number of websites and articles relating to nomenclatures or phages but never found anything explaining what T in T-4,5,6.. Phages mean in the Coliphages. Also, what does the number ...
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1answer
40 views

What disease / parasite / fungi has affected the mango in the picture?

The mango is found to have some kind of fungi / worm on it's surface . This has affected a huge number of mangoes in the same tree . It can be described as white and fibrous / weblike structure near ...
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Soil microbiology reference

I am after some good reference on Soil Microbe Ecology and Biology. I am a biophysicist, and have read the very gentle approach of "Life in the Soil" (James Nardi). Another book I have been ...
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1answer
160 views

Alternative dyes for Gram staining

In the Gram stain, is there any replacement for primary stain, secondary stain, decolourizer and mordant? What results will the replacements produce? I found that crystal violet can be replaced with ...
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2answers
280 views

Is there a favoured mathematical model of bacterial growth?

One of the most regularly measured phenotypes in bacterial synthetic biology is growth. Anyone who does wet lab microbiology or synthetic biology will have performed a growth curve measurement. ...
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Recommendations for a haemocytometer (phase-contrast)

I'm looking for recommendations for a haemocytometer that can be used with a phase-contrast microscope. I want to use it to quantify the vegetative and spore fraction in my samples. Can I use any ...
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How long would you expect a designed plasmid to remain in a population of E. coli after transformation?

When transforming E. coli with a genetically-modified plasmid, how quickly/often is this plasmid "lost" because of mutation, or horizontal-transfer, or "dilution" over reproduction ...
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Is publication an important criteria to get a post doc in microbial ecology?

I am a researcher in microbial ecology , currently searching for post doc in European countries. I have publication less than 1 impact factor. Will this hinder my chances of getting selected . Please ...
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