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Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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E. Coli growth inhibited in Powdered Honey

I ran into problem with my method validation since the E. Coli that I've spiked into my Honey Powder sample can't grow in the media. The media I've used is Merck CCA (Chromogenic Coliform Agar). I've ...
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growing bacteria [on hold]

You are growing Bacillus subtilis in nine 16,000-liter fermenters to produce enzymes for industrial use. The Bacillus cultures had been growing for 2 days when the cells in one of the fermenters lysed....
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Why does tetracycline have such a broad spectrum of activity?

What are the structural and chemical characteristics that make tetracycline uniquely broad spectrum? I understand it acts on the A-site of the prokaryotic ribosome, but there exist many ribosome-...
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Can vaccines move from person to person?

Can someone who has been given a vaccine "infect" another person with the virus used in that vaccine? For example, let's say Bill is vaccinated against tuberculosis. If Frank is exposed to Bill, is ...
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Antigen molecular mimicry

Let us consider a situation in which the body is attacked by a microbe, and the microbe is captured by the immune system for recognition of surface antigens. The surface antigen recognized mimics one ...
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1answer
23 views

Glass petri dish to replace plastic petri dish?

I never use a glass petri dish before, but I just wonder if I can use it to replace for plastic petri dish because it can reusable. The only thing I worry is , in a Fridge's user manual, it said that ...
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How do I determine an acceptable $F_0$ value in sterilization?

Here's a sample question: The sterilization process using moist heat is used to kill B.stearothermophilus by heating to 115 degrees C and holding it for 30 mins. For B.stearthermophilus, it ...
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(Reliable Source Needed): Why is the phospholipid membrane more permeable to hydrophobic molecules?

It is often said that it is difficult for hydrophilic molecules to pass through the phospholipid bilayer because of the hydrophobic core. However, it would seem logical to assume that it would also be ...
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How Would I Isolate and Amplify a Viral Enzyme?

what procedures would I use to isolate and amplify integrase? If I am trying to study the integrase enzyme which is found in HIV how would I 1) destroy the viral capsule to release its contents. 2) ...
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How do we know that mild forms of rabies are nonexistent?

Wikipedia's rabies article says: "Death usually occurs 2 to 10 days after first symptoms. Survival is almost unknown once symptoms have presented, even with the administration of proper and intensive ...
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1answer
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Why is there such in interest in antibiotics against Pseudomonas?

I keep finding information on anti-pseudomonal antibiotics, or information specifying the antibiotic has anti-pseudomonal activity. Is there anything peculiar to this bacterium when compared with ...
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What would ultimately happen to the bacteria if you leave a piece of cooked meat in a room temperature for a week or two? [closed]

I do know that bacteria spreads on the food even hours after leaving it on room temperature, but what would happen if the meat (food) stays there for a week or two? Does the bacteria die or eventually ...
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Virus plaque assay, pfu/ml calculation on plates with too little plaques formed

I am a student, so I hope it is okay to put this question here. I've done the virus plaque assay in a practical recently and have a few questions regarding the plaque count for pfu calculation. In ...
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Cell wall constitution of Schizosaccharomyces pombe

where is located the 1,3-α-glucan in the cell wall of Schizosaccharomyces pombe? Is it in the inner or outer layer? Thanks
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Why do so many parasites infect definitive and intermediate hosts rather than just one host?

Many parasites infect multiple host species, with one host species being the definitive host (where the parasite reproduces), and the other host species being the intermediate host (where the parasite ...
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2answers
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When two amoeba cells form from one amoeba, does spindle fibers form?

When two amoeba cells form from one amoeba, does spindle fibers form? Some sources say that spindle fibers do not form while an amoeba cell is undergoing cell division. If spindle formation does not ...
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Is it possible to contract the plague by kissing a wild chipmunk?"

I watched this cute video and I came to conclusion that the lady in the video is putting her life in danger. She kisses a wild chipmunk. As I know, they have fleas, and fleas have a black plague. ...
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Can people with AIDS/HIV be vaccinated?

If there is no immune system,it seems like vaccines wouldn't do much since there is no adaptive immune system to develop antibodies and memory cells. But can people with AIDS/HIV still be vaccinated? ...
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What is the purpose of the AraC gene in pGLO?

The AraC gene activates in pGLO from my understanding in the presence of arabinose. Arabinose is present as a metabolite from other metabolic in the e.coli cell. Is it that there's no termination ...
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Fluoroquinolone mitochondrial and genomic DNA adduct

What could be possible consequences of covalent bond mitochondrial and genomic DNA adduct by Fluoroquinolones, our study was done by Fluoroquinolone Toxicity Study NFP. All 50+ participates who took ...
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Microalgae with the highest carbon density

I am looking for about species of microalgae with the highest known carbon density. Specifically, I am looking for the highest carbon to volume and carbon to weight ratios when the algae is ...
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research on brain-eating amoeba

Are there any alternative treatments for brain-eating amoeba? I've spent about 6 hours trying to find information about this and haven't had any success.
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How much of dried microalgae is carbon by weight?

I am attempting to calculate the average concentration of elemental carbon per gram of dried freshwater microalgae for a climate change project. If it makes it to production, the algae will be grown ...
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Why are fungi and algae microbes?

Both fungi and algae have very small cells but so do most animals. Yet both of them are visible to naked eye just like other organisms. Why do we call them as microorganisms then ?
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For how long would a flu-contaminated parcel be an effective disease vector?

A parcel has been delivered and contaminated by a person who has the flu. For how long would the parcel be an effective disease vector?
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1answer
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What is the difference between transport proteins and effector proteins in gram-negative bacteria?

When considering pathogenic gram-negative bacteria, is there any difference between the function of transport proteins and effector proteins? Or are they of the same functionality? Any reference ...
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Can a plastic degrading bacteria project be recreated in a school lab environment?

I am looking for a science fair project and am wondering if this could be a good idea. I am in the 10th grade. I am not exactly sure of the materials needed since this is just the beginning stages of ...
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Could a human become infected with rabies in such a way that even prior vaccination wouldn't stop the infection?

Given the highly persistent nature of rabies after entering the brain, I was wondering whether certain ways of exposure to this virus could be risky even for a previously vaccinated individual. I ...
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Does turbidometeric method count cell number or cell mass?

Here it says that scattering is inversely proportional to cell number but also says that we can observe greater absorbance as cell number increases. So does turbidometeric method work on cell mass ...
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Can a plastic degrading bacteria science fair project be recreated in a school lab?

i am just looking for a science fair project and am fascinated by this topic. i was wondering if a project concerning the isolation of plastic degrading bacteria could be done in a school lab. I am in ...
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Could cyanobacteria thrive with very high CO2 concentrations and almost no oxygen to start with?

Has there been research to see if cyanobacteria could live in high levels of CO2, like in the atmosphere of Venus ? Because cyanobacteria caused the Great Oxygenation Event one might expect that they ...
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2answers
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Pox virus infection process

How does Pox virus duplicate it's genome? Does it bring DNA polymerase or RNA polymerase into the host cell?
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3answers
124 views

A Hadeeth on the fly

I think most biologists here on this site will not consider my question below to be unfit for posting on Biology SE. I came across this article about the hadeeth (a hadeeth is a saying of the ...
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1answer
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Protein Pathways, Superfamilie, Function database/website?

I'm going trough some old data - but I'm running into this problem that all website we used use is not existing anymore. What I have is a very long list of proteins with accession number (refSeq from ...
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Infant immunization

I know that polio vaccine consists of small dose of polio virus itself, which activates body's immunity against the disease. An infant is given a no of vaccines including chickenpox, tetanus, ...
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1answer
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Is it theoretically possible to safely eliminate most viruses in the atmosphere, hence preemptively cure all the viral diseases? [closed]

Could we create a genetically modified virus or bacteria (with inability to mutate into something dangerous for animals) that would quickly spread all over the planet and selectively kill most of the ...
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What is the number density of fungal spores per unit of volume of air?

Fungal spores are present all over in the air around us. I understand that they generally range in size from about one to one-hundred microns. What is the number density of fungal spores in air on a ...
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1answer
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can one be infected with Naegleria fowleri by taking Steam?

will Naegleria fowleri be present in the steam molecules. Many people take steam from hot water to get relief from sinus. I read somewhere that Naegleria fowleri gets killed over 70 C. So steam will ...
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1answer
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Why do neutrophils need to die after pathogen phagocytosis?

From referenced article below, neutrophils need to be removed because its granule contents and oxygen metabolites (used for killing phagocytosed pathogen) are harmful to the surrounding tissue. Thus, ...
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What is the difference between enrichment media and selective media?

Enrichment media These media are used to suppress commensal bacteria while allowing the pathogen to remain viable and grow. [Source: Ananthnarayan and Paniker's textbook of Microbiology.] ...
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Is egg white lysozyme different from bacterial lysozyme?

Someone told me that microbial lysozyme has effect on gram negative bacteria but egg white lysozyme only affect gram positive bacteria. Is it true?
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Freezing my dry encapsulated probiotics

Will my dry probiotics in capsules die if I freeze them for a long period of time?, even over a year. I'm talking about the regular capsules of probiotics that are purchased in little bottles to take ...
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How do the bacteria producing bacteriocins protect themselves from it?

Whenever there is Col plasmid in a bacteria it will produce bacteriocins which will kill other bacteria around it. Why will the bacteriocin not kill the bacteria which has produced it? How does the ...
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Why Cerebrospinal fluid is not heated for VDRL?

For performing VDRL of serum , we heat serum to inactivate complement proteins which may otherwise interfere , but why don't we do same for CSF even though it too has complement proteins in it? Is it ...
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1answer
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Why reaginic antibodies are absent in these types of syphilis?

According to Textbook of Microbiology and Immunology 2e, Subhash Chandra Parija, pg.no; 375 These(reaginic) antibodies do not appear in early primary syphilis, latent acquired syphilis of long ...
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Is there a publically available database of microbes that details what their wastes and food sources are

Is there a publically available database of microbes that contains what their wastes and chemical food sources are in addition to other traits. So that I could do like a sorted search for microbes ...
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I read somewhere that Amoeba Proteus eats up oil and natural janitor created by nature. Is that true?

When I was in Grade 7 I read somewhere in American Smithsonian Magazine that Amoeba Proteus is the Natural cleaner of Oil Disaster in Ocean. I cannot find the article again right now but I want to ...
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Is there a limit on how big a free living unicellular mono-nuclear organism can be?

When I looked for the largest protozoan I've found the name Syringammina fragilissima. According to wikipedia " .. Syringammina fragilissima, is among the largest known coenocytes, reaching up to 20 ...
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Reversal form of endosymbiotic theory?

Cell's organelles give us evidence that they might have been independent organisms on their own. Are there any single-cell microorganisms known to have gone so to say this way back as well, i.e. ...
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1answer
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When in Ampicillin degraded (gone) in liquid TB-media? Concerns about selectivity

Question: Specifically regarding Ampicillin; When growing cells in TB (terrific broth) for protein expression - when should I expect the ampicillin to be gone due to degradation by b-lactamases? (and ...