Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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28 views

Who is it and where? [closed]

Find some picture but idk what is it. any thoughts? https://i.stack.imgur.com/byJ9a.jpg
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Antibacterial soap impacts on septic system?

What impacts do antibacterial soaps have on septic systems? I know that septic systems rely heavily on bacteria (especially regarding the breakdown of wastes by bacteria), so it seems as though ...
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Can an Audio be sent into object inside Electron Microscopy Unit?

I just wondering about this technology inside a special lab called Electron Microscopy Unit. Can we send a live (sound) to the object (cell/electron) inside that device? I mean for not a recorded ...
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Do Microbes consume or break down Viruses?

The outer layer of the Virus is either a protein or a lipid so does that mean bacteria and fungi can potentially break them down with enyzme
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Is there software simulating virology experiments like LTspice XVII simulating electronic circuits? [closed]

I want a software if existed to simulate what happens when a virus attacks a bacteria or other organisms. It can change a parameter of the viruses.
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How are antibodies specific for a disease detected in the blood if everybody produces a different antibody for the same antigen?

To break the title down into parts: There exist serology tests that detect the amount of an antibody (Ab) against a specific pathogen/antigen. Every human produces their own Ab for a specific ...
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How to convert AU/ml of bacteriocin into zone of inhibition in mm?

My questions is about measuring antimicrobial activity against bacteria on petri plate. Is it possible to convert or somehow get zone of inhibition in millimetre from AU/ml?
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Why don't what I can define as “aerophilic viruses” establish colonies?

Throughout my life I have seen some bacterial and fungal colonies in various sizes and colors, mostly on food or in hot humid dirty environments. Putting bacteria and microfungi as well as other ...
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Ratio of bleach to water required to disinfect COVID-19?

I am confused about the needed ratio of bleach to water required to disinfect COVID-19 from surfaces. The guide on CDC states that: https://www.cdc.gov/coronavirus/2019-ncov/community/home/cleaning-...
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Relationship between bacteria size and temperature?

I'm, doing a research in a TOC (total organic carbon) degradation in a BAC (biologically active carbon) filter for the grey-water treatment. Recently, I started to wonder if there is a relation ...
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Commercially available chemically competent cells with LacI deletion?

I'm looking for a chemically competent cell line that I can purchase that has the LacI transcription factor deleted from its genome. Many have the lac operon deleted (B-galactosidase, i.e. LacZ is ...
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A question about microbes growing media

I was told in my microbiology course that blood agar can be put in GasPak system (anearobic) to grow strict anaerobes, but why would someone use blood-dependent bacteria in this experiment as these ...
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How are logarithmic trendline equations used to determine antibody concentrations in ELISA?

For example, say you have plotted a standard curve and managed to obtain a logarithimic trendline equation like: y = mln(x) - b And you are working with data that includes: a) standards,their ...
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what's the difference between cofactors and allosteric effectors?

"allosteric regulation (or allosteric control) is the regulation of an enzyme by binding an effector molecule at a site other than the enzyme's active site." does this imply that effector molecules ...
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sperm reaction of virus infected

we know when a human body cell infected by a virus , create interferon 1 to call other cells that surrounded it , but red blood cells couldn't do this because they loose their nuclear and other ...
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Paper shows 2 hours of high humidity and hot temperatures kills corona virus, does this stop the spread of the virus?

I found this paper (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2863430/) which says these corona virus strains live for months at 4 degrees C, 20% relative humidity. But at 40 degrees C and 80% ...
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Salt water swish for dental health [duplicate]

I was wondering as to why warm water is told for salt water gargle and not cold water, does warm water has more therapeutic effect? Also does salt really work, excess salty water might kill bacteria ...
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Does parasitism, one of Bacteria's lifestyle?

The parasite is an organism that lives in or on a host. It depends on its host for survival. Bacteria lives in decaying organic matter, within human organism (colon, oral cavity). It can be a ...
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Where can I obtain Sputnik virophage samples?

I'm working on a project where I'm trying to use virophages as viral vectors in introducing CRISPR-Cas proteins into other viruses. I was wondering where I might be able to find replication-defective ...
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Immunity to one's own microbiome

It seems that a human bite can be very dangerous, because of the myriads of bacterial species found in saliva. This leads to several questions that, perhaps, may have the same single answer. But, I'...
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A question about dicumarol

I know that dicumarol was discovered in moldy sweet clover as it caused hemorrhage in cattles, but if someone had thromboembolic disorders causing uncontrolled thrombosis,would it be fine to eat moldy ...
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Virus Mortality Selection

This question is related to the recent Wuhan outbreak. Currently, mortality rate for the virus is somewhere around 2-3%. This is much lower than say, SARS (9.5%) and MERS (34.5%). As a thought ...
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2answers
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What's the purpose of segregating a virus from the blood and growing it in the lab?

I heard that the scientist in Australia found a way to segregate the 2019 NovCoronovirus from the blood. Does this mean they are one step closer to the cure? Why is virus segregation crucial in ...
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Are there any known microbes capable of living in almost pure carbon dioxide?

Some bioengineering is investigated in high carbon dioxide environments. Are there any microbes like bacteria or Archaea capable of living and multiplying in such environments?
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What are the constraints on an operon?

From what I understand, the term operon is loosely defined to be a collection of genes that are found to be in the same proximity in a genome. Some definitions enforce that an operon is only ...
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Is PrEP used for anything other than HIV?

In the field of HIV prevention, PrEP is an abbreviation of pre-exposure prophylaxis. This is a very generic term. Are any diseases other than HIV prevented using a similar approach? Do they use the ...
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What makes iodine an effective antiseptic?

I'm thinking about tincture of iodine, potassium iodide (Lugol's), and povidone-iodine (PVP-I) specifically, which, as is my understanding, work by solubilizing elemental iodine in an aqueous solution....
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Can microbes become “overweight”?

Just out of some weird thoughts, can a microbe (any single-celled organisms, accepting answers for both prokaryotes and eukaryotes) ingest too much food (e.g. via absorbing too much involuntarily ...
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Using a magnetic stirrer as a makeshift shaking incubator?

I'm working in my high school's lab and need to culture bacteria in a broth culture. More specifically, I'm trying to harvest Bacillus endospores with the exhaustion method published in "Molecular ...
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Why do archaea survive better in extremes than bacteria?

Why have bacteria not evolved to fill the extreme environments to the same extent as archaea? What has enabled archaea to colonize these areas when bacteria do not?
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1answer
91 views

A theory about the possible connection between protists and first animalia

I learnt that organisms within Kingdom Animalia can be either microanimals or (nonmicro)animals. a microanimal is any Kingdom Animalia organism that in general cannot be seen by a human eye without ...
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Pathogen Modeling Program - Cold chain - Is there any model that allows you to change temperature at different times?

I am trying to predict the CFU (Colony-forming unit) or bacteria population size across time. There are programs where you can modelate the growing of bacteria and its population size through time ...
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Why did isopropyl alcohol not work as a disinfectant for E. coli in this experiment?

I recently performed a lab to test different concentrations (20-99%) of isopropyl alcohol and its inhibition of E. coli growth. My control was an antibacterial disk, and what I did was I dabbed the ...
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Using autoclaved store-bought distilled water for labwork?

I'm a high school student who's working on a molecular biology project in my school's lab. I need pure water for making culture media, buffers, running a BCA protein assay, running digests, cleaning ...
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How do mutations of viruses lead to drug resistance?

For instance, after starting zidovudine monotherapy against HIV, resistance develops against the drug because of a point mutation in the RNA transcriptase enzyme to which the drug binds. So how does ...
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1answer
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What is the simplest way to demonstrate the germicidal property of UV light?

This is intended for a high school project. I am familiar with the one that studies growth of e coli over time in a petri dish but I a bit concerned about as to how safe a demonstration of this nature ...
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Why does atrazine kill photosynthetic bacteria and not nonphotosynthetic bacteria?

Atrazine was found to bind to Qb, which is involved in electron transport to create ATP in photosynthetic bacteria. Why does this herbicide kill photosynthetic bacteria, while having little to no ...
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Can a bacterium express a virulence gene and not produce toxins and what may be the cause of this?

I am preparing a seminar on viable but non-culturable (VBNC) state in bacteria, mainly foodborne pathogens and I just saw this line in a journal article by Lothigius et al that "the expression of the ...
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Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
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Can anyone help identify these organism found in biofloc?

Can anyone help me with identifying what these organism is? It is found in my biofloc culture with shrimp Litopenaeus vannamei. I have been adding wheat as carbon source for my biofloc.
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Does more ATP present mean that a microbe can ferment more and decrease the pH? [closed]

I noticed that EMP produces 2 ATP and EDP produces 1 ATP. Does that mean that if more ATP is present that would give more energy for fermentation and lowering the pH of a system?
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Which culture media is suitable for cheek epithelial cells?

A month ago, I tried isolating cheek cells from saliva and the experiment was a success, however, I was wondering if it is possible to cultivate these cells in vitro. I'd say it's possible, but I wasn'...
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Can I sterilize the equipment for experiments without an autoclave?

If I don't have access to an autoclave, can I sterilize my equipment in a regular pot. If so, what time is needed at about 110°C (I measured inside) for a proper sterilization?
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Which bacteria takes the longest to double?

Currently I am writing a lab report on the enumeration of bacteria. E.coli takes about 30 mins to double. That is going from 1 bacteria to 2 bacteria. I want to know which bacteria takes the ...
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What is meant by Physarum polycephalum having 720 sexes?

There is currently some media hype about the slime mold Physarum polycephalum, which will soon be exhibited in the Paris zoo. This was my first time hearing about slime molds, but so far so good. ...
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How can Haemophilus influenzae survive and grow alone in the human body?

Haemophilus influenzae gets its name from the fact, that it requires nutrients from blood in order to grow. More specifically, I see it mentioned that in vitro they often exhibit the pheomenon that ...
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Cleaning Masterclave

When i finish operation. My masterclave has smell, I think has media moulecular bind into the surface and microbial can live in. My labs don't have SOP to clean it. I promote use detergent but wonder ...
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Lecithin in Tryptone Azlectin Tween (TAT) media

When I made TAT in tank and separate in chamber, I found precipitation in some chamber, not at all. I asked my site manager and her tell me it's lecithin and normal. But, in my oppinion, I think ...
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Why is synthesis of tetanospasmin advantageous to C. tetani?

The tetanospasmin is a neurotoxin synthesised by some strains of C. tetani. It is the factor causing tetanus, but what is its role for the bacteria itself? I do not believe the main objective of C. ...
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Assessing in soil biodiversity of arid area of US

I am considering assessing the microbial biodiversity (using metagenomics) in the soil of an arid area of Utah (Hanksville) that has dinosaur and other fossil remains. 1) Could it be argued that ...

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