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Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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Is it possible to remove cyanobacteria from rainwater without killing it?

My collected rainwater has a pronounced blue-green tint. I presume that this is due to the presence of Microcystis or another planktonic cyanobacterium. Is there a way to remove the cyanobacteria from ...
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How is the right amount of nucleotides synthesised during homologous recombination?

I'm reading about homologous recombination in the context of double-strand break repair. It seems like the trick is that around the break, homologous recombination uses a template duplex to generate ...
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Use of bacteriophages against viral infections [on hold]

I know that bacteriophages kill cell by essentially blowing up, and I also know that viruses are difficult to treat because they aren’t necessarily alive. Could bacteriophages be modified to attack ...
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Why some eggs are bile stained?

Why eggs of some helminths( Diphyllobothrium latum, Trichuris trichiura, Ascaris lumbricoides etc) are stained with bile while eggs of other helminths( Hymenolepis nana, Enterobius vermicularis etc.) ...
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qPCR: Accounting for different sample mass when interpreting and comparing Ct

I'm trying to use qPCR to quantify the amount of a specific bacteria species in patient sputum, but I don't really have the option to use consistent quantities of sputum to elute DNA from. The qPCR ...
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what is the characterization of mutant bacteria and how is it done?

I have a task to characterize protease producing bacterial mutants but I have failed to understand what it is and how it is done
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Volvox Cellular Reintegration

A couple of days ago, I answered this question over on worldbuilding.SE with some information about volvoxes, and commenter Liam Proven pointed out that one of the claims I made was dubious. I had ...
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particles in spirulina medium

Can any one suggest what are this particles in my spirulina growing medium? PH is between 10 and 11 and the medium is few months old. It turned green and stays green color even after I filter ...
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Trying to find the right plasmid expression vector for e. coli c600

I am very new to the concept of gene cloning and transformation. I am trying transfer the arenite oxidase gene cluster (9kb in length) to E. coli strain C600. I want the E. coli strain to express this ...
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can a naegleria fowleri amobea live in a bottle of water?

I want to know if this amoeba can live in a bottle of water that has been storaged for 10 years? I brought a bottle of water from the town of Lourdes in France 10 years ago. they sell the bottles of ...
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What happens to Bacterial Cells stored at 4 degrees?

4C is when water is most dense and is not so low as to cause ice crystal formation. For short term storage bacterial cultures are often simply kept in a fridge at 4C. I guess that this significantly ...
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Why do -ssRNA viruses need to do intermediate positive strand (antigenome) instead of just replicate it negative strands?

I was reading about RSV replication here https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185921/, but i cannot understand what is the reason for making a antigenome instead of just replicate it negative ...
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Thermophilic Total plate count with PCA with Triphenyl tetrazoliumchloride as supplement

Hello I'm am a fourth year microbiology analyst and I'm busy with finishing my final internship. During my final internship I have to complete a own assignment. the assignment I got was to find out if ...
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How does the accumulation of cyclic AMP lead to edema?

The antrax toxin is made up of three fractions. Protective antigen factor,edema factor and lethal factor. Protective antigen factor binds to the receptors of the host cell surface and facilitates the ...
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Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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Is it possible to see the microbes on your hand without using UV light or a microscope?

I know that black light (UV) is often used to see the microbes (colloquially referred to as germs) on people's hands but is there any way by which we can examine the microbes on people's hands without ...
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Why can't bacteriophages survive inside the human body thus negating the possibility of using them against bacteria?

I read about the therapeutic uses of bacteriophages in Pelczar's Microbiology. It was written that it is reasonable to think that bacteriophages could be used to kill pathogenic bacteria. However, the ...
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Do unique communities of bacteria form inside water storage in plants?

I assume that plants, like animals, have an ability to fight off bacteria that invade their cells (support: here and here. However, water often harbors quite a bit of bacteria in even unsuspecting ...
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We know that the hepatitis C virus can live on surfaces for at least six weeks. Maybe longer. The infectivity study ended after just six weeks; why?

Background A paper has found that the hepatitis C virus (HCV) can remain infective for at least six weeks on ordinary household surfaces. You can see the free full text, or a summary for busy ...
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A study found that the hepatitis C virus can live on surfaces for six weeks. Did they end the study before the seventh week began?

A study once found that the hepatitis C virus (HCV) can remain infective for six weeks on ordinary household surfaces. You can see the free full text, or a summary for busy clinicians. What happened ...
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What M9 stands for

Can anyone tell me what M9 stands for in M9 medium? I have tried googling but I still cannot find it.
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How does the intracellular accumulation of cyclic AMP cause edema in case of anthrax?

Edema factor is adenyl cyclase which is activated only inside the target cell. It leads to intracellular accumulation of cyclic AMP resulting in edema. How?
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E. Coli growth inhibited in Powdered Honey

I ran into problem with my method validation since the E. Coli that I've spiked into my Honey Powder sample can't grow in the media. The media I've used is Merck CCA (Chromogenic Coliform Agar). I've ...
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Why does tetracycline have such a broad spectrum of activity?

What are the structural and chemical characteristics that make tetracycline uniquely broad spectrum? I understand it acts on the A-site of the prokaryotic ribosome, but there exist many ribosome-...
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Can vaccines move from person to person?

Can someone who has been given a vaccine "infect" another person with the virus used in that vaccine? For example, let's say Bill is vaccinated against tuberculosis. If Frank is exposed to Bill, is ...
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Antigen molecular mimicry

Let us consider a situation in which the body is attacked by a microbe, and the microbe is captured by the immune system for recognition of surface antigens. The surface antigen recognized mimics one ...
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Glass petri dish to replace plastic petri dish?

I never use a glass petri dish before, but I just wonder if I can use it to replace for plastic petri dish because it can reusable. The only thing I worry is , in a Fridge's user manual, it said that ...
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How do I determine an acceptable $F_0$ value in sterilization?

Here's a sample question: The sterilization process using moist heat is used to kill B.stearothermophilus by heating to 115 degrees C and holding it for 30 mins. For B.stearthermophilus, it ...
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1answer
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How Would I Isolate and Amplify a Viral Enzyme? [closed]

what procedures would I use to isolate and amplify integrase? If I am trying to study the integrase enzyme which is found in HIV how would I 1) destroy the viral capsule to release its contents. 2) ...
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How do we know that mild forms of rabies are nonexistent?

Wikipedia's rabies article says: "Death usually occurs 2 to 10 days after first symptoms. Survival is almost unknown once symptoms have presented, even with the administration of proper and intensive ...
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1answer
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Why is there such in interest in antibiotics against Pseudomonas?

I keep finding information on anti-pseudomonal antibiotics, or information specifying the antibiotic has anti-pseudomonal activity. Is there anything peculiar to this bacterium when compared with ...
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Virus plaque assay, pfu/ml calculation on plates with too little plaques formed

I am a student, so I hope it is okay to put this question here. I've done the virus plaque assay in a practical recently and have a few questions regarding the plaque count for pfu calculation. In ...
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1answer
55 views

Why do so many parasites infect definitive and intermediate hosts rather than just one host?

Many parasites infect multiple host species, with one host species being the definitive host (where the parasite reproduces), and the other host species being the intermediate host (where the parasite ...
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When two amoeba cells form from one amoeba, does spindle fibers form?

When two amoeba cells form from one amoeba, does spindle fibers form? Some sources say that spindle fibers do not form while an amoeba cell is undergoing cell division. If spindle formation does not ...
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Is it possible to contract the plague by kissing a wild chipmunk?"

I watched this cute video and I came to conclusion that the lady in the video is putting her life in danger. She kisses a wild chipmunk. As I know, they have fleas, and fleas have a black plague. ...
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Can people with AIDS/HIV be vaccinated?

If there is no immune system,it seems like vaccines wouldn't do much since there is no adaptive immune system to develop antibodies and memory cells. But can people with AIDS/HIV still be vaccinated? ...
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What is the purpose of the AraC gene in pGLO?

The AraC gene activates in pGLO from my understanding in the presence of arabinose. Arabinose is present as a metabolite from other metabolic in the e.coli cell. Is it that there's no termination ...
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Fluoroquinolone mitochondrial and genomic DNA adduct

What could be possible consequences of covalent bond mitochondrial and genomic DNA adduct by Fluoroquinolones, our study was done by Fluoroquinolone Toxicity Study NFP. All 50+ participates who took ...
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research on brain-eating amoeba

Are there any alternative treatments for brain-eating amoeba? I've spent about 6 hours trying to find information about this and haven't had any success.
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Why are fungi and algae microbes?

Both fungi and algae have very small cells but so do most animals. Yet both of them are visible to naked eye just like other organisms. Why do we call them as microorganisms then ?
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For how long would a flu-contaminated parcel be an effective disease vector?

A parcel has been delivered and contaminated by a person who has the flu. For how long would the parcel be an effective disease vector?
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What is the difference between transport proteins and effector proteins in gram-negative bacteria?

When considering pathogenic gram-negative bacteria, is there any difference between the function of transport proteins and effector proteins? Or are they of the same functionality? Any reference ...
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Can a plastic degrading bacteria project be recreated in a school lab environment?

I am looking for a science fair project and am wondering if this could be a good idea. I am in the 10th grade. I am not exactly sure of the materials needed since this is just the beginning stages of ...
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Could a human become infected with rabies in such a way that even prior vaccination wouldn't stop the infection?

Given the highly persistent nature of rabies after entering the brain, I was wondering whether certain ways of exposure to this virus could be risky even for a previously vaccinated individual. I ...
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1answer
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Does turbidometeric method count cell number or cell mass?

Here it says that scattering is inversely proportional to cell number but also says that we can observe greater absorbance as cell number increases. So does turbidometeric method work on cell mass ...
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Can a plastic degrading bacteria science fair project be recreated in a school lab?

i am just looking for a science fair project and am fascinated by this topic. i was wondering if a project concerning the isolation of plastic degrading bacteria could be done in a school lab. I am in ...
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2answers
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Could cyanobacteria thrive with very high CO2 concentrations and almost no oxygen to start with?

Has there been research to see if cyanobacteria could live in high levels of CO2, like in the atmosphere of Venus ? Because cyanobacteria caused the Great Oxygenation Event one might expect that they ...
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Pox virus infection process

How does Pox virus duplicate it's genome? Does it bring DNA polymerase or RNA polymerase into the host cell?
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A Hadeeth on the fly

I think most biologists here on this site will not consider my question below to be unfit for posting on Biology SE. I came across this article about the hadeeth (a hadeeth is a saying of the ...
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Protein Pathways, Superfamilie, Function database/website?

I'm going trough some old data - but I'm running into this problem that all website we used use is not existing anymore. What I have is a very long list of proteins with accession number (refSeq from ...