Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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16 views

What's this parasite?

Any microbiologists or parasitologists recognize this? It's from my pet lizard and the vet's never seen it before.
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What is the biological basis of coronavirus infection? [closed]

I tried googling and researching about it but I'm kind of lost as to what "Biological Basis" actually means. I found a lot of definitions but none of them actually made sense to me. I can't seem to ...
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How can I obtain a uniform bacterial sample?

I'm currently doing a research project wherein I will be testing the antibiotic resistance of a bacterial sample. Due to a risk of contamination during transport, I'm not allowed to request a uniform ...
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ID request - some kind of mold?

Any ideas what is this? From very moldy water that was used to boil beets, the largest blob is about 34 microns in diameter.
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What fraction of human cells gets infected during a viral infection?

As I understand, if a cell gets infected during a viral infection then it eventually dies. If an individual does not die of an infection, the percentage of cells that gets infected in the course of ...
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Why viruses “die” outside the host?

Why viruses "die" outside the host after sufficient time? I want to know the biological process behind it. What role being exposed to air plays in virus destruction? What role sunlight plays in the ...
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When purifying plasmid DNA after cloning, do you get endogenous plasmid in addition to your vector of interest?

Since bacteria naturally contain plasmids, when you do a cloning experiment and purify the plasmids at the end, do you get plasmid naturally present in that bacteria in addition to your vector? The ...
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Can DNA rings, i.e. plasmids, form as Möbius strips?

I know that plasmids can be present in a coiled form, which keeps the DNA strands together when they degenerate, by forming catenases. I was wondering, however, whether it has been documented to ...
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How effective is rinsing in removing microbes from surfaces

Microbes including viruses stay alive on surfaces depending upon the type of the surface like steel, wood, plastic, glass, marble etc. How effective is rinsing these surfaces with tap water in ...
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Can I use a Flask Weight Ring to increase RPM in an orbital shaking incubator?

I am working on a device that goes on top of the flask. This reduces the maximum RPM that I can use. Therefore I am wondering if I can use a Flask Weight Ring for Erlemeyer flasks in a orbital ...
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How can Saccharomyces cerevisiae live in a dried state for long periods of time?

I want to know how can the Saccharomyces can live for so long in dried form. I am talking about the dried yeast used in baking .
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Can/have bacteria been engineered to express ACE 2 receptors?

In light of SARS-COV-2 binding to the ACE 2 protein in human cells, I was wondering if any bacteria exist or could exist that possess this membrane protein. If not, do you believe that an ACE 2 like ...
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Humic Acid and PCR

Quite a few papers claim humic acid is an inhibitor of PCR reactions. I understand this is true when working with soil microbes, but how does it qualify to be a PCR inhibitor in general (i.e when not ...
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What is a general timeline to replace stainless steel SS 316 pipes in food industry?

Does the dairy and/or food industry (specifically but not limited to the USA)considers to replace their stainless steel SS 316 or SS 304 pipes after say 30 years or so? Could someone please share ...
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characterising bacterial growth characteristics, recommendations?

To characterise a bacterias growth habits, we can obviously grow it in various medias and check the optical density at certain time intervals (with parallel). I understand this, and have no problems ...
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How does lactic acid cause muscle twitching?

It is well-known that lactic acid buildup (often caused by workouts) causes muscle twitching. Does anyone know HOW lactic acid achieves that effect?
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What is the principle behind Microagglutination test (MAT)?

Could some one please help me understand the principle behind the MAT. Also, is it only used to detect leptospira or can it be used to detect other pathogens as well? Kindly share links to papers if ...
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identificaation of the correct fragments with desired nucleotide sequences using probes

in making of rDNA, my text book has a point that is written above(the question). what i don't get is if we already know what the nucleotide sequence is why do we need to take out the gene having the ...
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Induce plasmid acquisition in bacteria to make them susceptible to antibiotics

Is it possible to use plasmids as a way of creating antibiotic susceptibility in bacteria. For example; in Microbiology, an introduction 13th edition by Pearson , Page 230 "the researchers traced the ...
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Mathematical model describing nutrient uptake rate as a function of population of microbial community

I'm looking for a mathematical function of the rate of nutrient uptake rate, in terms of population.
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Can single-cell organisms have through-holes? (Are donut-cells a thing?)

I've watched a YouTube video about microorganisms, which featured a shot (below) of a single-cell organism, that looked like it had several through-holes in its body. Somehow I have a pretty steady ...
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What factors affect the rate of siderophore production by microbes?

Microbes produce siderophores which are chelators and bind to iron. This is how microbes absorb iron from soil or rock. What causes microbes to increase or decrease the rate of production of ...
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I need to identify the genus of this colony just by its morphology, can somebody help me?

it was grown on TSA it's a mesophilic bacteria. (it was contaminated by E. coli, so ignore the clearer and smoother colonies).
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50 views

What is the disadvantage of circular DNA?

I have searched online and there is little information about the disadvantage of a circular DNA. Since I know that with chromosome arrangement like human, we have telomere that held the DNA strands ...
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60 views

By what physiological mechanism do we not feel microbes (bacteria, etc.) living on our skin?

Background I know our bodies have a handful of ways to threshold our awareness of sensory stimuli: Neural density Sensory acuity I assume really tiny stimuli could fit between receptors e.g., ...
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70 views

Why do coliform bacteria smell so bad?

I have done multiple cultures of E. coli bacteria and related species over the course of my education. Almost every time, they had this revolting smell. However, other organisms used for genetics ...
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Antibacterial soap impacts on septic system?

What impacts do antibacterial soaps have on septic systems? I know that septic systems rely heavily on bacteria (especially regarding the breakdown of wastes by bacteria), so it seems as though ...
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How are antibodies specific for a disease detected in the blood if everybody produces a different antibody for the same antigen?

To break the title down into parts: There exist serology tests that detect the amount of an antibody (Ab) against a specific pathogen/antigen. Every human produces their own Ab for a specific ...
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How to convert AU/ml of bacteriocin into zone of inhibition in mm?

My questions is about measuring antimicrobial activity against bacteria on petri plate. Is it possible to convert or somehow get zone of inhibition in millimetre from AU/ml?
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Why don't what I can define as “aerophilic viruses” establish colonies?

Throughout my life I have seen some bacterial and fungal colonies in various sizes and colors, mostly on food or in hot humid dirty environments. Putting bacteria and microfungi as well as other ...
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Ratio of bleach to water required to disinfect COVID-19?

I am confused about the needed ratio of bleach to water required to disinfect COVID-19 from surfaces. The guide on CDC states that: https://www.cdc.gov/coronavirus/2019-ncov/community/home/cleaning-...
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Relationship between bacteria size and temperature?

I'm, doing a research in a TOC (total organic carbon) degradation in a BAC (biologically active carbon) filter for the grey-water treatment. Recently, I started to wonder if there is a relation ...
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Commercially available chemically competent cells with LacI deletion?

I'm looking for a chemically competent cell line that I can purchase that has the LacI transcription factor deleted from its genome. Many have the lac operon deleted (B-galactosidase, i.e. LacZ is ...
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A question about microbes growing media

I was told in my microbiology course that blood agar can be put in GasPak system (anearobic) to grow strict anaerobes, but why would someone use blood-dependent bacteria in this experiment as these ...
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How are logarithmic trendline equations used to determine antibody concentrations in ELISA?

For example, say you have plotted a standard curve and managed to obtain a logarithimic trendline equation like: y = mln(x) - b And you are working with data that includes: a) standards,their ...
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sperm reaction of virus infected

we know when a human body cell infected by a virus , create interferon 1 to call other cells that surrounded it , but red blood cells couldn't do this because they loose their nuclear and other ...
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Paper shows 2 hours of high humidity and hot temperatures kills corona virus, does this stop the spread of the virus?

I found this paper (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2863430/) which says these corona virus strains live for months at 4 degrees C, 20% relative humidity. But at 40 degrees C and 80% ...
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Salt water swish for dental health [duplicate]

I was wondering as to why warm water is told for salt water gargle and not cold water, does warm water has more therapeutic effect? Also does salt really work, excess salty water might kill bacteria ...
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Does parasitism, one of Bacteria's lifestyle?

The parasite is an organism that lives in or on a host. It depends on its host for survival. Bacteria lives in decaying organic matter, within human organism (colon, oral cavity). It can be a ...
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Where can I obtain Sputnik virophage samples?

I'm working on a project where I'm trying to use virophages as viral vectors in introducing CRISPR-Cas proteins into other viruses. I was wondering where I might be able to find replication-defective ...
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Immunity to one's own microbiome

It seems that a human bite can be very dangerous, because of the myriads of bacterial species found in saliva. This leads to several questions that, perhaps, may have the same single answer. But, I'...
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A question about dicumarol

I know that dicumarol was discovered in moldy sweet clover as it caused hemorrhage in cattles, but if someone had thromboembolic disorders causing uncontrolled thrombosis,would it be fine to eat moldy ...
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Virus Mortality Selection

This question is related to the recent Wuhan outbreak. Currently, mortality rate for the virus is somewhere around 2-3%. This is much lower than say, SARS (9.5%) and MERS (34.5%). As a thought ...
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What's the purpose of segregating a virus from the blood and growing it in the lab?

I heard that the scientist in Australia found a way to segregate the 2019 NovCoronovirus from the blood. Does this mean they are one step closer to the cure? Why is virus segregation crucial in ...
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Are there any known microbes capable of living in almost pure carbon dioxide?

Some bioengineering is investigated in high carbon dioxide environments. Are there any microbes like bacteria or Archaea capable of living and multiplying in such environments?
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What are the constraints on an operon?

From what I understand, the term operon is loosely defined to be a collection of genes that are found to be in the same proximity in a genome. Some definitions enforce that an operon is only ...
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Is PrEP used for anything other than HIV?

In the field of HIV prevention, PrEP is an abbreviation of pre-exposure prophylaxis. This is a very generic term. Are any diseases other than HIV prevented using a similar approach? Do they use the ...
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What makes iodine an effective antiseptic?

I'm thinking about tincture of iodine, potassium iodide (Lugol's), and povidone-iodine (PVP-I) specifically, which, as is my understanding, work by solubilizing elemental iodine in an aqueous solution....
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Can microbes become “overweight”?

Just out of some weird thoughts, can a microbe (any single-celled organisms, accepting answers for both prokaryotes and eukaryotes) ingest too much food (e.g. via absorbing too much involuntarily ...
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Using a magnetic stirrer as a makeshift shaking incubator?

I'm working in my high school's lab and need to culture bacteria in a broth culture. More specifically, I'm trying to harvest Bacillus endospores with the exhaustion method published in "Molecular ...

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