The Stack Overflow podcast is back! Listen to an interview with our new CEO.

Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

Filter by
Sorted by
Tagged with
1
vote
1answer
124 views

What is meant by Physarum polycephalum having 720 sexes?

There is currently some media hype about the slime mold Physarum polycephalum, which will soon be exhibited in the Paris zoo. This was my first time hearing about slime molds, but so far so good. ...
2
votes
0answers
28 views

How can Haemophilus influenzae survive and grow alone in the human body?

Haemophilus influenzae gets its name from the fact, that it requires nutrients from blood in order to grow. More specifically, I see it mentioned that in vitro they often exhibit the pheomenon that ...
0
votes
0answers
19 views

Pathogenic bacteria in surface drinking water resources

I am trying to understand the interaction between pathogenic bacteria themselves and if there are other bacteria or microorganisms that graze them in fresh water aquatic systems. I want to know a ...
0
votes
0answers
21 views

Cleaning Masterclave

When i finish operation. My masterclave has smell, I think has media moulecular bind into the surface and microbial can live in. My labs don't have SOP to clean it. I promote use detergent but wonder ...
0
votes
0answers
4 views

Lecithin in Tryptone Azlectin Tween (TAT) media

When I made TAT in tank and separate in chamber, I found precipitation in some chamber, not at all. I asked my site manager and her tell me it's lecithin and normal. But, in my oppinion, I think ...
-2
votes
0answers
17 views

Regarding scallop Theorem

The Scallop theorem states that to achieve propulsion at low Reynolds number in Newtonian fluids a swimmer must deform in a way that is not invariant under time-reversal ( from Wikipedia ) here I don'...
1
vote
0answers
21 views

Why is synthesis of tetanospasmin advantageous to C. tetani?

The tetanospasmin is a neurotoxin synthesised by some strains of C. tetani. It is the factor causing tetanus, but what is its role for the bacteria itself? I do not believe the main objective of C. ...
0
votes
0answers
19 views

DNAse in bacterial transformation experiment

In recipient cells of a bacterial transformation experiment, what is the purpose of DNAse? Would we expect nothing on the plate and would this be considered a negative control?
0
votes
0answers
18 views

Assessing in soil biodiversity of arid area of US

I am considering assessing the microbial biodiversity (using metagenomics) in the soil of an arid area of Utah (Hanksville) that has dinosaur and other fossil remains. 1) Could it be argued that ...
0
votes
0answers
12 views

How do I prepare a Basal medium for autotrophic mutant creation

Minimal medium (MM) was prepared by adding 2.0 g sodium nitrate (NaNO3) to 1 L of basal medium (BM) fol- lowing Correll et al. (1987). Chlorate resistant sectors (CRSs) were generated on two media i.e....
0
votes
1answer
36 views

Bacterial Growth Formula

What are the units of $N$: the colonies of bacteria or the viable cell count? This is in regards to the bacterial growth formula used during serial dilutions, $$ N =N(0) e^{kt} \quad . $$
4
votes
1answer
51 views

Autoclaving media Question

1.When I autoclave media should I close the cap tightly or slightly? I put all tools in plastic bag when I autoclave, should I close slightly the plastic and when it finish I close tightly the ...
0
votes
0answers
37 views

Are most pathogenic bacteria facultative anaerobes?

I know that S. aureus and most gram-negative rods are facultative anaerobes. In terms of number of species, are most human pathogen-associated bacteria facultative anaerobes?
0
votes
0answers
19 views

Which species of rotifer is this?

If found this in a moss sample.
0
votes
1answer
21 views

How could be the concentration of airborne pathogens in a specific indoor space be measured?

I wonder if it is possible to measure the concentration of airborne pathogens in a specific indoor space in order to extract a percentage value. This value would be helpful to determine specific ...
6
votes
2answers
75 views

Is there a minimum load below which an infectious agent will not cause disease?

Suppose a single smallpox virus is injected in an human adult's body. Will it cause disease in the host? Is there a minimum microbial load below which it will not cause disease?
21
votes
2answers
4k views

Why we don't have vaccination against all diseases which are caused by microbes?

People can be vaccinated against certain diseases. Principle of vaccination is to use live attenuated load or inactivated. My question is - why we dont have vaccination against all diseases which are ...
3
votes
0answers
60 views

What disease does Saccharopolyspora erythraea cause?

For an examination assignment I have to find a disease caused by the bacterial species Saccharopolyspora erythraea, but I have searched the internet and have found no report of patients being infected ...
0
votes
0answers
11 views

What specific criteria do you use to choose E.coli strain for the experiments? What is the most standard strain?

For my specific case, I would like to know what plasmid to use to express two particular proteins (one of them being the GFP reporter) with its specific promoters. But more important than the answer ...
0
votes
0answers
25 views

E. coli & aspartate

I am familiar with the linkage between aspartate/ligand binding, receptor methylation, and flagellar behavior re. style of locamotion, but I do not know what the bacterium does with the aspartate it ...
1
vote
1answer
34 views

Water sample preservation

I am taking my students to collect water samples from different bodies of water. These bodies of water include the local river, a park pond, and water accumulation from recent rain. Once we gather the ...
1
vote
1answer
45 views

Growth rate oscillation in the a population of non-interacting bacteria

I have come across this theoretical paper claiming that if you start a batch culture from a single E. coli cell, the exponential growth rate of the population defined as $$k = \frac1N \frac{dN}{dt}$$ ...
1
vote
1answer
56 views

Is bacillus strain 2-9-3 really 250 million year old?

I've read that in the last decade (I believe) some scientists discovered a microorganism, which they dubbed bacillus strain 2-9-3, in a crystal of salt that they think is 250 million years old and ...
2
votes
0answers
23 views

Diffussion coefficient of blood

What it the diffusion coefficient of blood in, e.g. water? It is needed for microchannel length calculations I have found a bit different nubmers: 0.94 and 1.66 microns^2/ms, but not sure
1
vote
0answers
11 views

Is there any double staining method to visualise separate colour for plant tissue and fungal tissue?

Is there any double staining method for developing separate colour on fungal tissue and plant tissue when the said 2 types of tissues are intermingled? Unlike my previous question, this question not ...
0
votes
1answer
19 views

How DNA probe binds

I am studying about southern hybridization now and I've a doubt.After the DNA has been fragmented using restriction enzymes and obtained on nitrocellulose blot , it is still double stranded (the ...
0
votes
1answer
27 views

How do carbon 13:12 ratios confirm the biological origin of oldest microfossils?

I was reading this article about how Schopf and Valley’s findings in 2017 confirmed that the 3.5 billion–year-old microfossils found in the Apex chert of Western Australia are indeed remnants of ...
1
vote
1answer
40 views

Can exposure to blood make pathogens resistant to the immune system?

If a drop of my blood is dropped into a pool of pathogens. Does that act as "reverse-vaccination" for said pathogens? I see this it as a potential risk for bacteria/viruses to compete or get ...
1
vote
0answers
22 views

Species diversity in cheese and yoghurt?

There have been studies on the number of species on humans and in various other environments. I was wondering how many species including: viruses, bacteria, yeasts, and fungi inhabit ordinary farm ...
0
votes
0answers
50 views

What enzyme or bacteria could dissolve plastic?

What enzyme or bacteria could dissolve plastic aside from acetone? Preferably, low-cost and environmentally friendly. Any suggestions would be helpful. I'm targeting for thin plastic (low-density ...
4
votes
1answer
160 views

Why is glycerol supplied/used as 87% solution?

While preparing glycerol stocks of my transformed bacterial cells (DH5alpha) the protocol I had to follow was that I would make one 50% glycerol stock (500µl culture, 500µl glycerol 50%) and another ...
1
vote
0answers
49 views

Are olfactive indicators always the result of inadequacy in sanitation?

Question If you can smell the scent of food in a thoroughly cleaned container, does that imply trace amounts of food particles remain? Scope It goes without saying that our sense of smell is ...
4
votes
1answer
58 views

Is it unusual for populations of archaea to be found living inside trees?

The NPR News article and podcast Getting Fire From A Tree Without Burning The Wood begins with A scientist walks up to a cottonwood tree, sticks a hollow tube in the middle and then takes a lighter ...
0
votes
0answers
15 views

How protozoa spawns in newly formed ponds?

How big protozoa like Paramecium caudatum, Amoeba proteus, Euglena viridis etc. appear in newly created ponds of water? Suppose there was rain, and pond was formed. Or earth, dead leaves, live leaves ...
1
vote
1answer
70 views

Can a pathogen be totally resistant to the human immune system?

Can a pathogenic organism be totally resistant to the human immune system? For the purpose of this question, the organism in question must cause a disease. Examples of organisms that would qualify as ...
0
votes
1answer
15 views

Salt Bridge for Microbial Fuel Cell: Full or Partly Full?

I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (...
0
votes
0answers
22 views

What strain of Arthrospira (Spirulina) is this?

Can anyone help me to identify this strain of Arthrospira (Spirulina)
0
votes
0answers
18 views

Methods to degrade Bacillus thuringiensis cell wall

I'm looking for cell lysis methods for Bacillus thuringiensis in order to extract a protein. I considered using enzymes to degrade the cell wall, but itt seems that some well-known wall-degrading ...
0
votes
0answers
43 views

Found this moving inside S. cerevisiae, can someone tell me what is this?

This is my first post here. I found this in a microbiology practice and my teacher had no idea. S. cerevisiae dyed with methylene blue. Is the one in the middle; you can see a small thing moving ...
1
vote
1answer
66 views

How could pharmaceutical metabolizing bacteria be used to synthesize them?

Lets say I have a pharmaceutical "P". It can be broken down by enzymes of one strain of bacteria into two molecules one of which can be further metabolized enzymatically by a different strain of ...
0
votes
0answers
17 views

what are best methods for mrsa screening in hospital?

methods for screening MRSA are based on PCR (mecA detection) and MRSA latex test and culture. I need to know sensitive, specific and cost-effective methods for diagnosis MRSA infection on one work day ...
0
votes
1answer
37 views

Can you increase the microbiota in an insect?

I know you can suppress the microbiota of an insect with antibiotics, but I´m am looking for a way to increase it. For example, can I feed an insect with something that helps boost the bacteria like ...
0
votes
0answers
18 views

How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?

I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
0
votes
0answers
18 views

Pits, symplastic or apoplastic pathway?

After water has moved through the endodermis through the Casparian Strip, water continues to move down the water potential gradient into the xylem vessels through the pits, is movement through the ...
1
vote
0answers
28 views

DNA replication in $ E.coli $

$ E.coli $ has circular DNA which I guess implies one strand forms the outer circle and the other the inner one. So, is there a way to know if the replicated DNA forms the outer or inner circle? In ...
0
votes
0answers
15 views

Virulence Factors of Francisella tularensis

After completing research on the virulence factors of Francisella tularensis, I was wondering about a few specific structures/toxin and whether these are virulence factors of Francisella tularensis or ...
6
votes
1answer
137 views

Why do bulls-eye rashes look like they do?

People infected with Lyme Disease often present with an erythema migrans ("migrating redness") rash. Most often, these rashes are in the shape of a bulls-eye. Rash image. Presumably, this is a ...
1
vote
1answer
46 views

How is Pseudomonas aeruginosa diagnosed?

I know that P. aeruginosa is cultured on an agar plate, but which media or assays make it distinct from other Gram-negative bacteria?
1
vote
2answers
80 views

Could a strong magnet field repel the body before damaging it?

Could a magnetic field be strong enough to damage the body or erase the brain? Blood is slightly diamagnetic, so if the field was strong enough, could it repel a body evenly? Could a diamagnetic ...
0
votes
0answers
43 views

Is an uninoculated control (slant) catalase positive or negative?

The catalase test is a laboratory test utilized in identifying organisms which produce the enzyme catalase. Is an uninoculated control (slant test only) catalase positive or negative? I am assuming ...