Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

Filter by
Sorted by
Tagged with
-2
votes
0answers
32 views

Is our data about DNA purely empirical?

I was curious about what makes some things living. The concept of a virus prompted me to think about that. I read that a virus is a very simple organism with only DNA in it. This DNA is the "...
0
votes
1answer
38 views

What are the constraints on an operon?

From what I understand, the term operon is loosely defined to be a collection of genes that are found to be in the same proximity in a genome. Some definitions enforce that an operon is only ...
0
votes
1answer
42 views

Is PrEP used for anything other than HIV?

In the field of HIV prevention, PrEP is an abbreviation of pre-exposure prophylaxis. This is a very generic term. Are any diseases other than HIV prevented using a similar approach? Do they use the ...
1
vote
0answers
31 views

What makes iodine an effective antiseptic?

I'm thinking about tincture of iodine, potassium iodide (Lugol's), and povidone-iodine (PVP-I) specifically, which, as is my understanding, work by solubilizing elemental iodine in an aqueous solution....
0
votes
0answers
53 views

Can microbes become “overweight”?

Just out of some weird thoughts, can a microbe (any single-celled organisms, accepting answers for both prokaryotes and eukaryotes) ingest too much food (e.g. via absorbing too much involuntarily ...
0
votes
0answers
17 views

Using a magnetic stirrer as a makeshift shaking incubator?

I'm working in my high school's lab and need to culture bacteria in a broth culture. More specifically, I'm trying to harvest Bacillus endospores with the exhaustion method published in "Molecular ...
-1
votes
1answer
44 views

Why do archaea survive better in extremes than bacteria?

Why have bacteria not evolved to fill the extreme environments to the same extent as archaea? What has enabled archaea to colonize these areas when bacteria do not?
0
votes
1answer
63 views

A theory about the possible connection between protists and first animalia

I learnt that organisms within Kingdom Animalia can be either microanimals or (nonmicro)animals. a microanimal is any Kingdom Animalia organism that in general cannot be seen by a human eye without ...
0
votes
0answers
5 views

Pathogen Modeling Program - Cold chain - Is there any model that allows you to change temperature at different times?

I am trying to predict the CFU (Colony-forming unit) or bacteria population size across time. There are programs where you can modelate the growing of bacteria and its population size through time ...
1
vote
0answers
61 views

Why did isopropyl alcohol not work as a disinfectant for E. coli in this experiment?

I recently performed a lab to test different concentrations (20-99%) of isopropyl alcohol and its inhibition of E. coli growth. My control was an antibacterial disk, and what I did was I dabbed the ...
3
votes
2answers
52 views

Using autoclaved store-bought distilled water for labwork?

I'm a high school student who's working on a molecular biology project in my school's lab. I need pure water for making culture media, buffers, running a BCA protein assay, running digests, cleaning ...
8
votes
4answers
5k views

How do mutations of viruses lead to drug resistance?

For instance, after starting zidovudine monotherapy against HIV, resistance develops against the drug because of a point mutation in the RNA transcriptase enzyme to which the drug binds. So how does ...
1
vote
1answer
34 views

What is the simplest way to demonstrate the germicidal property of UV light?

This is intended for a high school project. I am familiar with the one that studies growth of e coli over time in a petri dish but I a bit concerned about as to how safe a demonstration of this nature ...
1
vote
0answers
18 views

Why does atrazine kill photosynthetic bacteria and not nonphotosynthetic bacteria?

Atrazine was found to bind to Qb, which is involved in electron transport to create ATP in photosynthetic bacteria. Why does this herbicide kill photosynthetic bacteria, while having little to no ...
3
votes
2answers
62 views

Can a bacterium express a virulence gene and not produce toxins and what may be the cause of this?

I am preparing a seminar on viable but non-culturable (VBNC) state in bacteria, mainly foodborne pathogens and I just saw this line in a journal article by Lothigius et al that "the expression of the ...
0
votes
0answers
27 views

Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
0
votes
0answers
17 views

Can anyone help identify these organism found in biofloc?

Can anyone help me with identifying what these organism is? It is found in my biofloc culture with shrimp Litopenaeus vannamei. I have been adding wheat as carbon source for my biofloc.
1
vote
0answers
30 views

Does more ATP present mean that a microbe can ferment more and decrease the pH? [closed]

I noticed that EMP produces 2 ATP and EDP produces 1 ATP. Does that mean that if more ATP is present that would give more energy for fermentation and lowering the pH of a system?
1
vote
0answers
18 views

Which culture media is suitable for cheek epithelial cells?

A month ago, I tried isolating cheek cells from saliva and the experiment was a success, however, I was wondering if it is possible to cultivate these cells in vitro. I'd say it's possible, but I wasn'...
2
votes
1answer
41 views

Can I sterilize the equipment for experiments without an autoclave?

If I don't have access to an autoclave, can I sterilize my equipment in a regular pot. If so, what time is needed at about 110°C (I measured inside) for a proper sterilization?
2
votes
1answer
74 views

Which bacteria takes the longest to double?

Currently I am writing a lab report on the enumeration of bacteria. E.coli takes about 30 mins to double. That is going from 1 bacteria to 2 bacteria. I want to know which bacteria takes the ...
2
votes
1answer
710 views

What is meant by Physarum polycephalum having 720 sexes?

There is currently some media hype about the slime mold Physarum polycephalum, which will soon be exhibited in the Paris zoo. This was my first time hearing about slime molds, but so far so good. ...
2
votes
0answers
36 views

How can Haemophilus influenzae survive and grow alone in the human body?

Haemophilus influenzae gets its name from the fact, that it requires nutrients from blood in order to grow. More specifically, I see it mentioned that in vitro they often exhibit the pheomenon that ...
0
votes
0answers
23 views

Cleaning Masterclave

When i finish operation. My masterclave has smell, I think has media moulecular bind into the surface and microbial can live in. My labs don't have SOP to clean it. I promote use detergent but wonder ...
0
votes
0answers
5 views

Lecithin in Tryptone Azlectin Tween (TAT) media

When I made TAT in tank and separate in chamber, I found precipitation in some chamber, not at all. I asked my site manager and her tell me it's lecithin and normal. But, in my oppinion, I think ...
1
vote
0answers
25 views

Why is synthesis of tetanospasmin advantageous to C. tetani?

The tetanospasmin is a neurotoxin synthesised by some strains of C. tetani. It is the factor causing tetanus, but what is its role for the bacteria itself? I do not believe the main objective of C. ...
0
votes
0answers
20 views

Assessing in soil biodiversity of arid area of US

I am considering assessing the microbial biodiversity (using metagenomics) in the soil of an arid area of Utah (Hanksville) that has dinosaur and other fossil remains. 1) Could it be argued that ...
0
votes
0answers
13 views

How do I prepare a Basal medium for autotrophic mutant creation

Minimal medium (MM) was prepared by adding 2.0 g sodium nitrate (NaNO3) to 1 L of basal medium (BM) fol- lowing Correll et al. (1987). Chlorate resistant sectors (CRSs) were generated on two media i.e....
0
votes
2answers
53 views

Bacterial Growth Formula

What are the units of $N$: the colonies of bacteria or the viable cell count? This is in regards to the bacterial growth formula used during serial dilutions, $$ N =N(0) e^{kt} \quad . $$
4
votes
1answer
57 views

Autoclaving media Question

1.When I autoclave media should I close the cap tightly or slightly? I put all tools in plastic bag when I autoclave, should I close slightly the plastic and when it finish I close tightly the ...
0
votes
0answers
44 views

Are most pathogenic bacteria facultative anaerobes?

I know that S. aureus and most gram-negative rods are facultative anaerobes. In terms of number of species, are most human pathogen-associated bacteria facultative anaerobes?
0
votes
0answers
20 views

Which species of rotifer is this?

If found this in a moss sample.
0
votes
1answer
21 views

How could be the concentration of airborne pathogens in a specific indoor space be measured?

I wonder if it is possible to measure the concentration of airborne pathogens in a specific indoor space in order to extract a percentage value. This value would be helpful to determine specific ...
6
votes
2answers
86 views

Is there a minimum load below which an infectious agent will not cause disease?

Suppose a single smallpox virus is injected in an human adult's body. Will it cause disease in the host? Is there a minimum microbial load below which it will not cause disease?
21
votes
2answers
4k views

Why we don't have vaccination against all diseases which are caused by microbes?

People can be vaccinated against certain diseases. Principle of vaccination is to use live attenuated load or inactivated. My question is - why we dont have vaccination against all diseases which are ...
3
votes
0answers
60 views

What disease does Saccharopolyspora erythraea cause?

For an examination assignment I have to find a disease caused by the bacterial species Saccharopolyspora erythraea, but I have searched the internet and have found no report of patients being infected ...
0
votes
0answers
12 views

What specific criteria do you use to choose E.coli strain for the experiments? What is the most standard strain?

For my specific case, I would like to know what plasmid to use to express two particular proteins (one of them being the GFP reporter) with its specific promoters. But more important than the answer ...
0
votes
0answers
26 views

E. coli & aspartate

I am familiar with the linkage between aspartate/ligand binding, receptor methylation, and flagellar behavior re. style of locamotion, but I do not know what the bacterium does with the aspartate it ...
1
vote
1answer
43 views

Water sample preservation

I am taking my students to collect water samples from different bodies of water. These bodies of water include the local river, a park pond, and water accumulation from recent rain. Once we gather the ...
1
vote
1answer
46 views

Growth rate oscillation in the a population of non-interacting bacteria

I have come across this theoretical paper claiming that if you start a batch culture from a single E. coli cell, the exponential growth rate of the population defined as $$k = \frac1N \frac{dN}{dt}$$ ...
1
vote
1answer
68 views

Is bacillus strain 2-9-3 really 250 million year old?

I've read that in the last decade (I believe) some scientists discovered a microorganism, which they dubbed bacillus strain 2-9-3, in a crystal of salt that they think is 250 million years old and ...
2
votes
0answers
23 views

Diffussion coefficient of blood

What it the diffusion coefficient of blood in, e.g. water? It is needed for microchannel length calculations I have found a bit different nubmers: 0.94 and 1.66 microns^2/ms, but not sure
1
vote
0answers
12 views

Is there any double staining method to visualise separate colour for plant tissue and fungal tissue?

Is there any double staining method for developing separate colour on fungal tissue and plant tissue when the said 2 types of tissues are intermingled? Unlike my previous question, this question not ...
0
votes
1answer
21 views

How DNA probe binds

I am studying about southern hybridization now and I've a doubt.After the DNA has been fragmented using restriction enzymes and obtained on nitrocellulose blot , it is still double stranded (the ...
0
votes
1answer
30 views

How do carbon 13:12 ratios confirm the biological origin of oldest microfossils?

I was reading this article about how Schopf and Valley’s findings in 2017 confirmed that the 3.5 billion–year-old microfossils found in the Apex chert of Western Australia are indeed remnants of ...
1
vote
1answer
40 views

Can exposure to blood make pathogens resistant to the immune system?

If a drop of my blood is dropped into a pool of pathogens. Does that act as "reverse-vaccination" for said pathogens? I see this it as a potential risk for bacteria/viruses to compete or get ...
1
vote
0answers
22 views

Species diversity in cheese and yoghurt?

There have been studies on the number of species on humans and in various other environments. I was wondering how many species including: viruses, bacteria, yeasts, and fungi inhabit ordinary farm ...
0
votes
0answers
50 views

What enzyme or bacteria could dissolve plastic?

What enzyme or bacteria could dissolve plastic aside from acetone? Preferably, low-cost and environmentally friendly. Any suggestions would be helpful. I'm targeting for thin plastic (low-density ...
4
votes
1answer
171 views

Why is glycerol supplied/used as 87% solution?

While preparing glycerol stocks of my transformed bacterial cells (DH5alpha) the protocol I had to follow was that I would make one 50% glycerol stock (500µl culture, 500µl glycerol 50%) and another ...
1
vote
0answers
50 views

Are olfactive indicators always the result of inadequacy in sanitation?

Question If you can smell the scent of food in a thoroughly cleaned container, does that imply trace amounts of food particles remain? Scope It goes without saying that our sense of smell is ...