Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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2answers
70 views

In what stage of bacterial growth can penicillin inhibit the growth of the cellular wall?

The bacterial growth has 3 main stages: lag-phase, log-phase and stationary phase. I was wondering in which one of them penicillin can inhibit the growth of the cellular wall of bacteria and why. I ...
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Can the metatranscriptomics replace the approach of functional metatranscriptomics/functional metagenomics?

While metatranscriptomics reveals information about the expression of genes and their functions too, Functional metatranscriptomics (https://www.nature.com/articles/ismej201167) allows the ...
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1answer
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Is the stuffy environment due to using masks all day, helping in spread corona virus in people?

I came across a research that stated, the most disease ridden place in a toilet is actually the air dryer. The hot and moist microcosm that develops around the dryer due to frequent use helps in ...
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1answer
47 views

Are there any organisms that perform photosynthesis-like processes with thermal energy alone?

The closest I can find are thermophiles, but it seems they use the chemicals present in the hot environment for energy, not the heat itself.
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1answer
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Nutrient agar as the medium for antibiotic susceptibility testing

I couldn't get Mueller Hinton agar to conduct antibiotic susceptibility testing. I'm thinking of just choosing nutrient agar as the growth medium for the bacteria Staphylococcus epidermidis. Will ...
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1answer
47 views

What does T and the numbers mean in T coliphages?

I have searched a number of websites and articles relating to nomenclatures or phages but never found anything explaining what T in T-4,5,6.. Phages mean in the Coliphages. Also, what does the number ...
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1answer
29 views

What disease / parasite / fungi has affected the mango in the picture?

The mango is found to have some kind of fungi / worm on it's surface . This has affected a huge number of mangoes in the same tree . It can be described as white and fibrous / weblike structure near ...
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Soil microbiology reference

I am after some good reference on Soil Microbe Ecology and Biology. I am a biophysicist, and have read the very gentle approach of "Life in the Soil" (James Nardi). Another book I have been ...
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1answer
122 views

Alternative dyes for Gram staining

In the Gram stain, is there any replacement for primary stain, secondary stain, decolourizer and mordant? What results will the replacements produce? I found that crystal violet can be replaced with ...
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2answers
254 views

Is there a favoured mathematical model of bacterial growth?

One of the most regularly measured phenotypes in bacterial synthetic biology is growth. Anyone who does wet lab microbiology or synthetic biology will have performed a growth curve measurement. ...
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0answers
24 views

Recommendations for a haemocytometer (phase-contrast)

I'm looking for recommendations for a haemocytometer that can be used with a phase-contrast microscope. I want to use it to quantify the vegetative and spore fraction in my samples. Can I use any ...
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1answer
119 views

How long would you expect a designed plasmid to remain in a population of E. coli after transformation?

When transforming E. coli with a genetically-modified plasmid, how quickly/often is this plasmid "lost" because of mutation, or horizontal-transfer, or "dilution" over reproduction ...
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1answer
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Is publication an important criteria to get a post doc in microbial ecology?

I am a researcher in microbial ecology , currently searching for post doc in European countries. I have publication less than 1 impact factor. Will this hinder my chances of getting selected . Please ...
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141 views

Why can't certain nucleoside analogs be used to label bacterial RNA?

A 2020 publication in Nucleic Acids Research 1 includes the following passage: A variety of nucleoside analogs have been developed for metabolic RNA labeling in various eukaryotic cells (9–16). Among ...
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Concentration/molarity of lichen acids (particularly oxalic acid) in soil/rock substrates?

I am trying to design an experiment testing the efficacy of lichen acids to solubilize and mobilize nutrients from minerals in a lunar regolith simulant. My research so far indicates that oxalic acid ...
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1answer
55 views

Skeletisation in space

A space pod containing an astronaut drifts out to space and cannot be rescued. The space pod contains no insects, and the only bacteria present are those originally brought in by the astronaut. There ...
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0answers
27 views

Need help designing an enzyme immunoassay (EIA)

I have a FITC-labelled DNA sequence and an anti-FITC antibody, however this antibody is not conjugated with HRP or any other enzyme. I work in a lab with limited finances, so is there a way I could ...
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1answer
67 views

If silver nanoparticles kill bacteria by penetrating the cell membrane, why don't they also kill human cells on contact?

Apparently, the silver from the nanoparticles can penetrate the cell wall of bacteria and kill them from the inside. Why, then, doesn't the same happen to human cells, e.g. the skin on my foot when I ...
4
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1answer
353 views

How is SARS-CoV-2 'deactivated' for some Covid vaccines (for example Covaxin)? [closed]

Some Covid vaccines like Covaxin employ a 'Whole-Virion Inactivated Vero Cell'. How is the virion 'deactivated' for the vaccine?
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1answer
28 views

Reliable recipe to make DIY yeast slants

Does anyone have any good ideas on a reliable way to activate basic grocery store yeast? I realize this is a total beginner's question. I've built a small yeast ranch at home with my kid, and we're ...
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0answers
38 views

How do tardigrades defend themselves from predators?

Tardigrades or water bears are well known for their ability to enter into a cryptobiotic state which allows them to survive extreme environmental conditions such as extreme temperatures or radiation. ...
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1answer
19 views

When should I use cryofixation and chemical fixation?

We know that the technique used in TEM sample preparation involves multiple steps, one of the most important of them is fixation. Fixation can be of two types: Cryofixation, that suggests that the ...
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2answers
43 views

What material is permeable for plant roots, but “impermeable” for fungi & bacteria?

I'm designing an experiment where the roots of the plant will grow in a sterile environment while the shoot will grow in a non-sterile grow room. For this reason the environments of the shoot and the ...
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0answers
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What tests are available to detect C. Botulinum/Botulinum Toxin in fermented meats?

I am interested in the preparation of fermented dry-sausages (salamis, summer-sausages, etc) where due to the humid anaerobic atmosphere inside the salami. It is a good environment for C.Botulinum ...
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1answer
904 views

Can anyone ID this microorganism?

I found this microorganism in a wet moss sample taken from my backyard in Richmond, Virginia (USA). I took some pictures of it at 40x, 100x, and 250x. I'm not entirely sure what it is, but I think it'...
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Is it possible for a prion to have a short incubation period ie 1 week or 1 month?

I know that all prions so far have long incubation periods but is there a possibility for one to have a short incubation period, is there any evidence that suggests this is possible. Thank you
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Creating a phylogenetic tree from my selected publicly-available sequences (WGS) in NCBI

I'm currently writing a paper on the comparison of virulence genes for a group of bacteria. I got my data from publicly-available whole genome sequences in NCBI. Now, I want to create a phylogenetic ...
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4answers
153 views

Are there any organisms that are made of more than one (~5-12) cell?

Prokaryotes and eukaryotes are unicellular, made of one cell. Great. Eukaryotes are unicellular or multicellular. But the typical examples of multicellular eukaryotes we have are made of, often, ...
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1answer
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Winogradsky column and hydrogen sulfide

For a science experiment for school, I have set up 15 Winogradsky columns, and will be looking at the microbial growth inside the columns over a period of 25 days. I have read that, due to gas ...
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11 views

Eukaryotic cell lysate-based protein expression efficiency

What is average efficiency of eukaryotic cell lysate-based protein expression systems in terms of (mg of protein expressed) / (mg of lysate) ?
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1answer
102 views

Are the y-axis values informative when performing biolayer interferometry?

I am currently reading an this article by Alexandra C. Walls et. al. I would like to ask a question about a graph that is being used in the article and I wanted to know if my analysis was correct. I ...
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0answers
24 views

Aerosolised particle released during autoclaving

During autoclave heat-up, the inside chamber's air is released in order to be displaced by steam. Because of that, many modern autoclaves are equipped with filters at the release outlet. The ...
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1answer
54 views

Can Euchromatin convert into Heterochromatin?

I know that Heterochromatin can convert into Euchromatin but is the reverse possible? If yes, then How?
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1answer
150 views

What keeps mitochondria from multiplying out of control?

What keeps mitochondria from multiplying out of control and killing the cell? According to endosymbiotic theory, mitochondria were once free-living bacteria that got ingested but not metabolized by ...
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5answers
9k views

Can bacteria be killed by purely physical trauma?

In a question over on Skeptics Stack Exchange, I half-jokingly listed "blunt force trauma" as a means by which a bacterium could be killed. That makes me wonder if it really is only a joke ...
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2answers
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Can a bacterium infect another bacterium?

I researched about it by searching on Google and reading some bacteriological articles, but I did not get any answer. I also asked some of my teachers, and they were also a bit confused. Some said ...
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1answer
377 views

circular pattern of mold on quince

Could someone explain why mold on my quinces grows in well separated circles around a center? To make clear, what I mean I post the picture
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1answer
45 views

bunsen vs meker for best sterile field [closed]

I need one bunsen and i have a friend that sells me a bunsen-meker burner. I see that bunsen-meker burners have more flame diameter, so should have more diameter of sterile field, but i dont know XD ...
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1answer
167 views

Are plasmids found in eukaryotes? [closed]

There are some who says plasmids are found in some eukaryotes but is it like scientifically proven?
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1answer
30 views

Low-cost simple water testing for faecal contamination

I live in Indonesia and there are lots of sewage pipes discharging directly from homes/restaurants/etc directly into the river. It's also common to wash clothes and so on, so there is likely detergent ...
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1answer
37 views

Can viruses be deformed?

Viruses are created in their fully-formed state and do not grow. But one thing has been bugging me lately: can a virus be deformed, e.g. in order to fit into an opening slightly smaller than itself? ...
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114 views

What is the adjective for PHAGE? [closed]

if the adjective of the noun BACTERIUM is 'bacterial' and that of VIRUS is 'viral', what is the adjective for (BACTERIO)PHAGE? Is 'phagial' accepted? Thank you
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What types of cells HPV can infect? [closed]

I am wondering if there is any new information on which cells HPV is able to infect. Is it still limited to squamous cells or are there other cells such as columnar cells it is able to infect?
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1answer
90 views

Can anyone identify this microorganism?

I was examining some rainwater from my backyard in Richmond, VA, and found this microorganism. I took some pictures of it at 400x and 1000x (see below). The microorganism is attached to some moss and ...
0
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1answer
353 views

Can a second mass extinction by microbes happen?

Science Daily reports that the mass extinction at the end of Permian period happened by the Methanosarcina archaea wiping out 90% of species: Methane-producing microbes may be responsible for the ...
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1answer
54 views

What is the difference among these compounds?

I'll preface this question by making clear that I'm not not well versed in biology. Anyway, within the context of biocides/antimicrobial products using them as active substances, what is the ...
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1answer
28 views

types of cells in a pharyngeal specimen

I would like to ask what type of cells should someone expect in a pharyngeal smear, in a person, who is infected with a viral infection. Thank you a lot!
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1answer
212 views

Will obligate aerobic bacteria die if placed in a buffer solution even in the presence of oxygen?

The electron transport chain in aerobic bacteria functions by pumping H+ out of the cell to establish a concentration gradient. So if the bacteria are placed in a buffer solution having pH equal to ...
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What is the difference between chrysophytes and protozoa?

Amoeba is present both as a protozoa and as a chrysophytes.How to understand ,with which defining feature to which group to classify into?

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