Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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Directed mutation of viruses in response to drugs

For instance, after starting zidovudine monotherapy against HIV, resistance develops against the drug because of a point mutation in the RNA transcriptase enzyme to which the drug binds. So how does ...
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Using autoclaved store-bought distilled water for labwork?

I'm a high school student who's working on a molecular biology project in my school's lab. I need pure water for making culture media, buffers, running a BCA protein assay, running digests, cleaning ...
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Inoculating agar plates with microbes suspended in sterilised water?

We were having a problem with our experiment (culturing microbes on homemade nutri agar) so we decided to change our set-up. It already cost us quite some money. So, we saw this procedure. Can anyone ...
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What is the simplest way to demonstrate the germicidal property of UV light?

This is intended for a high school project. I am familiar with the one that studies growth of e coli over time in a petri dish but I a bit concerned about as to how safe a demonstration of this nature ...
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How is a bacterial strain defined?

When a species of bacteria is referred to by its strain, are they a clone of single founder or is a certain amount variation allowed?
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Bacterial Growth Formula

What are the units of $N$: the colonies of bacteria or the viable cell count? This is in regards to the bacterial growth formula used during serial dilutions, $$ N =N(0) e^{kt} \quad . $$
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characterizing organism during the second phase of multiple fission

Does an organism turn multicellular during the second phase of multiple fission, as the structure obeys all the needs to be a multicellular organism... or is it both multi and uni cellular as ...
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Why does atrazine kill photosynthetic bacteria and not nonphotosynthetic bacteria?

Atrazine was found to bind to Qb, which is involved in electron transport to create ATP in photosynthetic bacteria. Why does this herbicide kill photosynthetic bacteria, while having little to no ...
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Can a bacterium express a virulence gene and not produce toxins and what may be the cause of this?

I am preparing a seminar on viable but non-culturable (VBNC) state in bacteria, mainly foodborne pathogens and I just saw this line in a journal article by Lothigius et al that "the expression of the ...
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If someone were to die on the moon, would their body decay?

I heard that the footprints of Neil Armstrong are still there, so I was wondering if someone were to die there, would they remain preserved, too? If not how long would it take for them to decay?
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Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
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Which terms am I looking for to understand the biochemistry of Pavlovian Conditioning?

There is a biochemical basis for every brain activity. Which terms am I looking for to understand the biochemistry of Pavlovian Conditioning?
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Can a pathogen be totally resistant to the human immune system?

Can a pathogenic organism be totally resistant to the human immune system? For the purpose of this question, the organism in question must cause a disease. Examples of organisms that would qualify as ...
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1answer
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Can you increase the microbiota in an insect?

I know you can suppress the microbiota of an insect with antibiotics, but I´m am looking for a way to increase it. For example, can I feed an insect with something that helps boost the bacteria like ...
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Why do yeast ferment Equal sweetener

I had my students conduct a fermentation experiment manipulating sugar amounts (number of sugar packets in solution) and measuring CO₂ production via capture in a balloon and measuring circumference (...
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Can anyone help identify these organism found in biofloc?

Can anyone help me with identifying what these organism is? It is found in my biofloc culture with shrimp Litopenaeus vannamei. I have been adding wheat as carbon source for my biofloc.
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Does more ATP present mean that a microbe can ferment more and decrease the pH? [closed]

I noticed that EMP produces 2 ATP and EDP produces 1 ATP. Does that mean that if more ATP is present that would give more energy for fermentation and lowering the pH of a system?
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Which culture media is suitable for cheek epithelial cells?

A month ago, I tried isolating cheek cells from saliva and the experiment was a success, however, I was wondering if it is possible to cultivate these cells in vitro. I'd say it's possible, but I wasn'...
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1answer
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Which bacteria takes the longest to double?

Currently I am writing a lab report on the enumeration of bacteria. E.coli takes about 30 mins to double. That is going from 1 bacteria to 2 bacteria. I want to know which bacteria takes the ...
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1answer
19 views

Salt Bridge for Microbial Fuel Cell: Full or Partly Full?

I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (...
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1answer
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Can I sterilize the equipment for experiments without an autoclave?

If I don't have access to an autoclave, can I sterilize my equipment in a regular pot. If so, what time is needed at about 110°C (I measured inside) for a proper sterilization?
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Is iodised salt harmful to the lactofermentation process?

I was discussing sauerkraut recipes with someone on IRC a minute ago, and they warned me not to use iodised salt in the process. My first (and so far, the only successful) batch is one I made with ...
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Why should benzalkonium chloride (an antifungal agent) be used during your laundry machine's “rinse” cycle, not its “wash” cycle?

I'll first provide lots of background information. My actual question is at the very end. Background The spread of athlete's foot Athlete's foot is a fungal infection which is mildly contagious. ...
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1answer
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What is meant by Physarum polycephalum having 720 sexes?

There is currently some media hype about the slime mold Physarum polycephalum, which will soon be exhibited in the Paris zoo. This was my first time hearing about slime molds, but so far so good. ...
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How can Haemophilus influenzae survive and grow alone in the human body?

Haemophilus influenzae gets its name from the fact, that it requires nutrients from blood in order to grow. More specifically, I see it mentioned that in vitro they often exhibit the pheomenon that ...
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How could pharmaceutical metabolizing bacteria be used to synthesize them?

Lets say I have a pharmaceutical "P". It can be broken down by enzymes of one strain of bacteria into two molecules one of which can be further metabolized enzymatically by a different strain of ...
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Cleaning Masterclave

When i finish operation. My masterclave has smell, I think has media moulecular bind into the surface and microbial can live in. My labs don't have SOP to clean it. I promote use detergent but wonder ...
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Lecithin in Tryptone Azlectin Tween (TAT) media

When I made TAT in tank and separate in chamber, I found precipitation in some chamber, not at all. I asked my site manager and her tell me it's lecithin and normal. But, in my oppinion, I think ...
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Why is synthesis of tetanospasmin advantageous to C. tetani?

The tetanospasmin is a neurotoxin synthesised by some strains of C. tetani. It is the factor causing tetanus, but what is its role for the bacteria itself? I do not believe the main objective of C. ...
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Assessing in soil biodiversity of arid area of US

I am considering assessing the microbial biodiversity (using metagenomics) in the soil of an arid area of Utah (Hanksville) that has dinosaur and other fossil remains. 1) Could it be argued that ...
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Freezing my dry encapsulated probiotics

Will my dry probiotics in capsules die if I freeze them for a long period of time?, even over a year. I'm talking about the regular capsules of probiotics that are purchased in little bottles to take ...
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How do I prepare a Basal medium for autotrophic mutant creation

Minimal medium (MM) was prepared by adding 2.0 g sodium nitrate (NaNO3) to 1 L of basal medium (BM) fol- lowing Correll et al. (1987). Chlorate resistant sectors (CRSs) were generated on two media i.e....
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Water sample preservation

I am taking my students to collect water samples from different bodies of water. These bodies of water include the local river, a park pond, and water accumulation from recent rain. Once we gather the ...
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Autoclaving media Question

1.When I autoclave media should I close the cap tightly or slightly? I put all tools in plastic bag when I autoclave, should I close slightly the plastic and when it finish I close tightly the ...
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Are most pathogenic bacteria facultative anaerobes?

I know that S. aureus and most gram-negative rods are facultative anaerobes. In terms of number of species, are most human pathogen-associated bacteria facultative anaerobes?
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How is Pseudomonas aeruginosa diagnosed?

I know that P. aeruginosa is cultured on an agar plate, but which media or assays make it distinct from other Gram-negative bacteria?
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How could be the concentration of airborne pathogens in a specific indoor space be measured?

I wonder if it is possible to measure the concentration of airborne pathogens in a specific indoor space in order to extract a percentage value. This value would be helpful to determine specific ...
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325 views

Serological assays measuring antibody response

Given that an appropriate immune response to a bacteria may be thwarted in an individual, including not producing all of the antibodies which are known to occur in people who have been infected, or ...
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1answer
251 views

What concentration of hydrogen peroxide kills mold?

There are numerous articles online which claim that household 3% hydrogen peroxide can kill mold growing on household surfaces, yet I haven't managed to find scientific studies that show that. My ...
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Is there a minimum load below which an infectious agent will not cause disease?

Suppose a single smallpox virus is injected in an human adult's body. Will it cause disease in the host? Is there a minimum microbial load below which it will not cause disease?
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What disease does Saccharopolyspora erythraea cause?

For an examination assignment I have to find a disease caused by the bacterial species Saccharopolyspora erythraea, but I have searched the internet and have found no report of patients being infected ...
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1answer
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Can different Chromobactierum strains have completley different colony morphology?

In the analysis of an unknown bacterial broth I observed a gram negative rod genus. Upon plating I observed small, dark purple and convex colonies; corresponding presumably to Chromobactierum. After ...
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Why we don't have vaccination against all diseases which are caused by microbes?

People can be vaccinated against certain diseases. Principle of vaccination is to use live attenuated load or inactivated. My question is - why we dont have vaccination against all diseases which are ...
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1answer
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Does DHA increase the effects of clarithromycin?

I've read on this publication that every drug that induces CYP3A can reduce the effectiveness of clarithromycin. Clarithromycin inhibits CYP3A. But it does not say anything about the opposite ...
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1answer
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Does turbidometeric method count cell number or cell mass?

Here it says that scattering is inversely proportional to cell number but also says that we can observe greater absorbance as cell number increases. So does turbidometeric method work on cell mass ...
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What specific criteria do you use to choose E.coli strain for the experiments? What is the most standard strain?

For my specific case, I would like to know what plasmid to use to express two particular proteins (one of them being the GFP reporter) with its specific promoters. But more important than the answer ...
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E. coli & aspartate

I am familiar with the linkage between aspartate/ligand binding, receptor methylation, and flagellar behavior re. style of locamotion, but I do not know what the bacterium does with the aspartate it ...
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1answer
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Can exposure to blood make pathogens resistant to the immune system?

If a drop of my blood is dropped into a pool of pathogens. Does that act as "reverse-vaccination" for said pathogens? I see this it as a potential risk for bacteria/viruses to compete or get ...
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What happens to lactic acid I eat?

Let's say I drink some Kefir Milk, what happens to the lactic acid in the Kefir Milk that has entered my digestive system?