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Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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fimbriae under AFM

Can any one,please, recommend a protocol for watching bacteria with fimbriae under atomic force microscope? I have tried a lot but always there were no fimbriae i don't know why.
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Relation between OD with different microbial growth [on hold]

Equal volumes of cell suspensions of Escherichia coli, Saccharomyces cerevisiae, Streptococcus lactis and Mycoplasma pneumoniae, all have the same OD600nm = 0.50. Which cell suspension would have the ...
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Can I order yeast strains as an independent researcher? [on hold]

I have been studying rum manufacture and have tracked down several unique species and strains of yeast that can be valuable to my research. I recently contacted CBS to ask about purchasing some of ...
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In my DNA sequence for one sampling site there're W's; Y's; R's and K's?

I also just experienced the same thing with my DNA sequences, there are w's, Y', R', s' and k'. This is the very first time I've seen this. I am used to N but not these ones. Please kindly assist with ...
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Screening a environmental sample for three genes through running PCR reactions using these three primer sets and one negative control which one?

Would it be tuf and rrn genes since they are present since we are assuming the controls are working. Image below: https://docs.google.com/document/d/17GqdNXGCc9CmgyDSP5YRv8sv8za9QKk3PJwPDnDKUmE/edit?...
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when you are to non-culturally detect the presence of E. coli in the soil from a cow pasture?

You are to non-culturally detect the presence of E. coli in the soil from a cow pasture. You do this by extracting the total DNA from a sample of the soil and PCR amplifying with E. coli-specific ...
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You wish to make a 20 μl enzyme reaction cocktail that contains 120 μM MgCl2? [closed]

You wish to make a 20 μl enzyme reaction cocktail that contains 120 μM MgCl2. The concentration of your MgCl2 stock solution is 0.4 mM. How much of your MgCl2 stock would you have to add to your ...
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221 views

Organisms in food deposits

I have just discovered that my kitchen sink pipe has been leaking for quite some time (possibly months) which has resulted in water and food deposits collecting in my cupboard. I smeared some of the ...
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Is there a consensus on the best Machine Learning model for Antimicrobial Resistance (AMR) prediction?

Prediction of bacterial resistance/susceptibility to antimicrobials (from genotypic data) using Machine Learning methods is a problem that has started receiving interest in recent years. The ...
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Would removing proteins kill a virus? [on hold]

I was was wondering what would happen to a virus if we remove all the AUG in the RNA at the dna level, how would this effect a virus? I am aware it would kill a cellular creature for sure because ...
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297 views

Which is the reference 16S rRNA?

Recently, I've stumbled upon a fact, which hasn't bothered me for many years. The fact is that all universal 16S primers are written as "[FR][0-9]+" (in regex notation), that is they have a position ...
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For how long would a flu-contaminated parcel be an effective disease vector?

A parcel has been delivered and contaminated by a person who has the flu. For how long would the parcel be an effective disease vector?
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Difference between pili, fimbrae, cilia and flagella

What is the fundamental difference between all these extensions? What are their primary functions? Why did these different structures evolve -- wasn't it possible to have just a single type of ...
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3answers
15k views

Why do yeast ferment Equal sweetener

I had my students conduct a fermentation experiment manipulating sugar amounts (number of sugar packets in solution) and measuring CO₂ production via capture in a balloon and measuring circumference (...
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1answer
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If a bacterium had a protein on its surface that humans also have, would it cause an autoimmune disease?

Suppose that a bacterium happened to have a protein on its surface. This protein can also be found in the human body. If this bacterium were to then infect a human with an otherwise normal immune ...
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2answers
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Odds of finding superbug in soil?

There are tons of soil, rock, etc. being dug up, dug into, and moved all around the world each day. Paleontologists likewise regularly excavate fossils and both buried and frozen specimens, and ...
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116 views

Term of the type xxx-troph for a compound not used by an organism

A prototroph for compound X can make it A bradytroph grows faster if X is scavenged An auxotroph needs to scavenge X A hyperauxotroph lacks both the biosynthetic pathway and the transporters for X ...
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2answers
117 views

Storage of bacteria

Why are strains of bacteria stored when they are inactive (frozen)? What is the problem with storing growing cultures for long periods?
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205 views

Comparison between Transmission Electron Microscope (TEM) to Scanning Electron Microscope (SEM)

Can someone state the advantages of Transmission Electron Microscope (TEM) over Scanning Electron Microscope (SEM) and vice versa?
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Operon Repressors and activators

I know that the repressor when it binds to inducer it cannot bind to the operator and transcription goes on. As for the activator, it is made either active or inactive based on the environment and ...
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1answer
69 views

Is it possible to identify this microscopic particle?

So I was playing amateur scientist with my 11 year old. This photograph is from rainwater in a bird-feeding bowl in south England, at 300x. It looks biological, but I have no idea what this is. Is ...
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1answer
59 views

Action of tetracycline on 30s ribosomal subunits

My question concerns the mode of action of 30s ribosomal antibiotc inhibitors — tetracyclines. According to some online resources, the antibiotics enter the A-site, which prevents other tRNA from ...
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1answer
2k views

Count colonies in the right way

I use Rose bengal agar cloramfenicol for search yeast and molds for inclusion in petri plat. Which is the method correct for count? I'm not sure if i count everything or only the bigger one?
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1answer
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What do the text annotations in an electron micrograph mean?

I saw a photograph taken from an electron microscope, with some text. I want to know what is the meaning of these numbers and letters? Thank you! Regards.
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3answers
163 views

Is there any kind of antibiotic effective against fungi?

I know that antibiotics usually have properties affecting specifically bacterial cells, like by inhibiting peptidoglycan synthesis. but do any antibiotics exist affecting eukaryotic cells, like yeast ...
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synthesis of zn nanoparticle

Sir the protocol I followed where it has been written... 1st using lactobacillus in Mrs media (50 ml in 250 ml conical)..we allowed to incubate for 24 hrs then added 0.1M znSO4 (50ml) and 0.4 M NaOH(...
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1answer
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What makes an E.coli an E.coli, genotype or phenotype?

According to this paper, among 61 strains of E. coli they studied only 6% of the genes are common in all. Which means that the overwhelming majority of the genes are not shared. And wikipedia ...
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Why doesn't alum coagulate my cyanobacteria?

My collected rainwater has a pronounced blue-green tint which I believe to be due to the presence of cyanobacteria. I have been trying to remove the cyanobacteria using aluminium sulphate, but it ...
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1answer
69 views

Why are tardigrades so rare?

If tardigrades are so resilient and nearly immortal, why are they so rare when looking at samples of a pond or lake, where tardigrades should thrive? I've met people who've been studying protozoa and ...
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Difference between trichocyst and cilia of paramecium

I was reading about how paramecium move. I read that they move thanks to the structures called cilia and trichocysts. When I look for images, they both look very similar. I am familiar with cilia but ...
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1answer
104 views

How can fresh cavities form at the margins of sliver amalgam fillings on teeth?

Silver amalgam fillings predominantly contain silver a known bactericidal agent and mercury which a known toxin and has bactericidal property. So how is it that the plaque bacteria survive near the ...
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1answer
1k views

For people who work in microbiology labs, what software do you use to manage your strains?

It's just a small microbiology lab that currently records everything on paper, and there's quite few mutants as well. Is Excel commonly used for this sort of thing? Or is there a better software to ...
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2answers
776 views

Growing bacterial culture on gelatin

I'm very new to experimental biology and don't know much about it. So, i was looking to grow some bacteria in petri dishes and looked up how to do it. Unfortunately, I'm finding it difficult to get my ...
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Is it possible to remove cyanobacteria from rainwater without killing it?

My collected rainwater has a pronounced blue-green tint. I presume that this is due to the presence of Microcystis or another planktonic cyanobacterium. Is there a way to remove the cyanobacteria from ...
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How is the right amount of nucleotides synthesised during homologous recombination?

I'm reading about homologous recombination in the context of double-strand break repair. It seems like the trick is that around the break, homologous recombination uses a template duplex to generate ...
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Why some eggs are bile stained?

Why eggs of some helminths( Diphyllobothrium latum, Trichuris trichiura, Ascaris lumbricoides etc) are stained with bile while eggs of other helminths( Hymenolepis nana, Enterobius vermicularis etc.) ...
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1answer
22 views

qPCR: Accounting for different sample mass when interpreting and comparing Ct

I'm trying to use qPCR to quantify the amount of a specific bacteria species in patient sputum, but I don't really have the option to use consistent quantities of sputum to elute DNA from. The qPCR ...
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2answers
197 views

How is Coenzyme A Transported to the Matrix?

So, I've been researching cellular respiration on my own, and trying to keep track of most of the major processes. However, I do have one question left: I can't seem to find any sort of information ...
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what is the characterization of mutant bacteria and how is it done? [closed]

I have a task to characterize protease producing bacterial mutants but I have failed to understand what it is and how it is done
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Volvox Cellular Reintegration

A couple of days ago, I answered this question over on worldbuilding.SE with some information about volvoxes, and commenter Liam Proven pointed out that one of the claims I made was dubious. I had ...
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particles in spirulina medium

Can any one suggest what are this particles in my spirulina growing medium? PH is between 10 and 11 and the medium is few months old. It turned green and stays green color even after I filter ...
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Trying to find the right plasmid expression vector for e. coli c600

I am very new to the concept of gene cloning and transformation. I am trying transfer the arenite oxidase gene cluster (9kb in length) to E. coli strain C600. I want the E. coli strain to express this ...
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can a naegleria fowleri amobea live in a bottle of water?

I want to know if this amoeba can live in a bottle of water that has been storaged for 10 years? I brought a bottle of water from the town of Lourdes in France 10 years ago. they sell the bottles of ...
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1answer
411 views

Is abiogenesis possible today?

Life on earth started about 3.5 billion years ago. I would assume abiogenesis happened because the conditions were right. Would the current earth conditions allow for new abiogenesis and completely ...
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What happens to Bacterial Cells stored at 4 degrees?

4C is when water is most dense and is not so low as to cause ice crystal formation. For short term storage bacterial cultures are often simply kept in a fridge at 4C. I guess that this significantly ...
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Why do -ssRNA viruses need to do intermediate positive strand (antigenome) instead of just replicate it negative strands?

I was reading about RSV replication here https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185921/, but i cannot understand what is the reason for making a antigenome instead of just replicate it negative ...
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1answer
59 views

Is it possible to see the microbes on your hand without using UV light or a microscope?

I know that black light (UV) is often used to see the microbes (colloquially referred to as germs) on people's hands but is there any way by which we can examine the microbes on people's hands without ...
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Thermophilic Total plate count with PCA with Triphenyl tetrazoliumchloride as supplement

Hello I'm am a fourth year microbiology analyst and I'm busy with finishing my final internship. During my final internship I have to complete a own assignment. the assignment I got was to find out if ...
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4answers
283 views

What did the evolution of multicellular animals look like?

What did the evolution of multicellular animals look like? Aspects of this question include: (1) Are there any living organisms that might be helpful in visualizing "transitional forms" between ...
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How does the accumulation of cyclic AMP lead to edema?

The antrax toxin is made up of three fractions. Protective antigen factor,edema factor and lethal factor. Protective antigen factor binds to the receptors of the host cell surface and facilitates the ...