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Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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If an antiserum was raised in a buffalo by injecting it with monkey red blood cells, what would be the full name for this antiserum?

Im confused, i've attempted this & what i got is buffalo anti-monkey red blood cell antiserum. I'm not sure if this answer is right
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largest and smallest gram positive, gram negative and fungal pathogens

As I stumbled through this question: A 16-year-old female patient presented to her family physician with a complaint of an abnormal vaginal discharge and pruritus (itching). The patient ...
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Preparation of DNA ladder

how to prepare working stocks of 100 bp and 1 kb DNA ladder supplied by promega? the pack that we purchased contains a 6X loading dye. thanks!
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38 views

Is this panspermia mechanism feasible?

One episode of Cosmos: A Spacetime Odyssey describes a mechanism by which microbes could travel between planetary systems. As a star's orbit takes it around the galaxy, it occasionally passes through ...
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120 views

We know archea and eukarya are more closely related but endosymbiotic theory says eukarya have evolved from prokaryotes, justify the statements?

I am confused if eukaryotes have evolved from prokaryotes then how are they more closely related to archea?
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What is the difference between operator mutant and repressor mutant lambda viruses?

I wanted to know what the difference between the operator mutant and repressor mutant lambda bacteriophages is and how do they act on E. coli cells.
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Bacteria utilize short chain fatty acid as energy source

I'm doing research about the short chain fatty acid producing bacteria. I studied 7 short chain fatty acid (acetic propionic butyric isobutyric valeric isovaleric and captionic). and I found that the ...
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58 views

Bacteria without plasmid

I am currently working on antibiotic resistance and I would be very interested to know which bacteria has no plasmid, in a similar way than M. tuberculosis. This would help me a lot for my work and I ...
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81 views

How does gram-negative bacteria use high molecular weight starch if the membrane is only permeable to small molecular weights?

So one of my question is: The outer membrane of a gram negative bacterium contains pores that allow only molecules with a molecular weight less than 1,000 to cross. What does this bacterium need in ...
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333 views

Identification of protozoa under microscope

I observed maybe Protozoa from standing FRESH water and from slowly flowing FRESH water. I am complete dilettante. Can you tell what these creatures are? https://www.youtube.com/watch?v=6D5ck3zNJzA&...
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57 views

Can we investigate the cell biology of extinct microorganisms?

I know that there are microfossils, but not if we can we know about the organelles, genetics or macromolecules of the cells that left them behind.
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134 views

Can one type of bacteria cause several diseases? [closed]

If yes. Does it change its shape in every disease, or does it remain in the same shape and cause several diseases?
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Is a wild-type strain more “fit” than its mutant strains?

Question's context In the context of a presentation on mathematical models for antibiotic-resistance bacteria research, someone mentioned that wild type strain (WT) are expected to be more fit than ...
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determining horizontal gene transfer between two genomes?

I want to determine if there is horizontal gene transfer between two genomes, in particular I was to use the parametric methods described in wikipedia since I would most likely struggle to understand ...
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Is swimming pool water actually a weak topical medication?

I was inspired by this question. Given that the chlorine levels in swimming pool water are so high that they can seriously harm amphibians, it seems logical that the water should also kill various ...
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Contamination of Salmonella and Shigella in public internet cafes

I've read somewhere that the keyboard computer and computer mouses can house a lot of microbes especially in public internet cafes. ew Is it possible that Salmonella and Shigella could somehow be ...
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53 views

How do you culture Cutibacterium acnes?

I've been thinking of culturing Cutibacterium acnes (old name is Propionibacterium acnes) for a thesis topic of mine on the comparison of the efficacy of local antibacterial topical skin care products ...
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61 views

How to measure the length of mitochondria from z stack fluorescent microscopy image?

I have been working on yeast cells to analyse the effect of DNA damaging agents on mitochondrial structure. I have imaged my culture treated with MMS for a period of 6 hours and while observing the ...
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Can bacteria grow in rough, deteriorated indoor plastic chairs left in months of rain? (i.e. not made of resin plastic)

I have a problem with indoor plastic chairs (like classroom chairs with metal frames) left outside in the rain for months at my units. They are not made of resin plastic and the surface is noticeably ...
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What causes this shade in gel electrophoresis

Currently in uni I have recently done a exercise of extracting DNA from yeast cell. This is somewhat related to homework, but instead of asking about the answer im wanting to understand why there is a ...
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What is the meaning of multicellularity?

I can't understand what multicellularity is. Wikipedia states that any organism having many cells is multicellular. By this definition bacteria can also be multicellular. For example, cyanobacteria ...
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65 views

Is there any mechanism that prevents DNA of an eaten entity's cell from affecting that of own?

When we eat food I heard that our digestive enzymes disassemble the DNA of the eaten cells so that the DNA cannot affect us. Then, is there any mechanism (that prevents the eaten cell's DNA from ...
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Can different Chromobactierum strains have completley different colony morphology?

In the analysis of an unknown bacterial broth I observed a gram negative rod genus. Upon plating I observed small, dark purple and convex colonies; corresponding presumably to Chromobactierum. After ...
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Classification of ciliates found in city stream water

I recently collected some water along with some precipitations from a stream. It is quite shallow (less than a meter in depth) and its flow rate is slow, so that the precipitations won't get washed ...
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How to predict the behavior of bacteria?

You take a very large petri dish that contains a population of bacteria. The bacteria live in the petri dish and you periodically add food to the dish. You decide to test what happens when you ...
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How do (commensal and mutualist) microorganisms colonize the seminal vesicles?

Human's seminal fluid has it's own microbiome (Javurek et al. 2016). How do species of this microbiome (bacteria and eukaryotes) colonize the seminal vesicles? It is possible that different species ...
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239 views

Is it safe to use an egg incubator to grow bacterial cultures?

I've been using a small egg incubator to grow environmental bacteria in petri dishes. I always wear gloves and glasses when I handle the plates and do Gram staining. How likely is it that bacteria ...
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Would current-day human-initiated panspermia be effective?

With current technology, we are able to easily send a probe to the Alpha Centauri star system at a speed of about 20 km/s (velocity of voyager probe), which means it would take about 65000 years to ...
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98 views

Is it possible to make anaerobic media too reducing?

I've been struggling to grow an anaerobe (P. gingivalis), I take every precaution to keep the culture conditions free of oxygen, I was wondering if the conditions can be so reducing that they inhibit ...
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Does Penicillium roqueforti produce penicyllin?

I have recently discovered that blue cheese is produced with Penicillium roqueforti. As I know that some Penicillinum sp. produce penicyllin I wonder whether it is produced by P. roqueforti too? The ...
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1answer
498 views

Growing bacterial culture on gelatin

I'm very new to experimental biology and don't know much about it. So, i was looking to grow some bacteria in petri dishes and looked up how to do it. Unfortunately, I'm finding it difficult to get my ...
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212 views

What does the “Psi” in “Psi broth” stand for?

When making competent cells I've been using a medium called "Psi broth", which per liter consists of 20 g tryptone, 5 g yeast extract and 5 g MgSO4. Today I got curious about why it's called "psi". Is ...
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Microbroth dilution

I have performed MIC test for fungi candida albicans, Cryptococcus neoformans and Aspergillus fumigatus in a 96 well plate by broth microdilution method by drugs Amphotericin B and fluconazole.....
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60 views

Is it possible to produce bread using only microorganisms?

I am wondering if it is theoretically possible to produce bread using only microorganisms. If one lists all the chemical components, proteins fermentation time, etc., is it then doable to combine the ...
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Is one single antibody binding its antigen enough to elicit an immune response?

IVIG (intravenous immunoglobulin) is derived from plasma of more than 1000 donors. I wonder if in this preparation is one antibody present that recognize its antigen after infusion - if this is enough ...
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662 views

Sickle cell life span

How long do sickle red blood cells "live" before being broken down in phagocytosis? I had trouble doing a normal search as it brings up life span of those inflicted with the disease. Also, I have ...
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241 views

Can you use a microscope without an eyepiece? [closed]

Can you use a microscope without an eyepiece?
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2answers
106 views

How do chemists calculate the existence of billions of organisms?

So the packaging of Bio-Cultures Complex says that it contains a minimum of a billion viable organisms per capsule. How has such an estimate been drawn? Have they counted a smaller amount and then ...
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Relation between OD with different microbial growth

Equal volumes of cell suspensions of Escherichia coli, Saccharomyces cerevisiae, Streptococcus lactis and Mycoplasma pneumoniae, all have the same OD600nm = 0.50. Which cell suspension would have the ...
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146 views

Where do kombucha SCOBYs get nitrogen?

Kombucha can be brewed even with tiny amounts of inocculate, such as the little bit of sediment at the bottom of store-bought kombuchas. Over 1-2 weeks, this can grow into an inch thick film covering ...
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107 views

Test of antibiotic A for sensitivity as surrogate of antibiotic B

I am reading the WHO Global Antimicrobial Resistance Surveillance System (GLASS) - Manual for Early Implementation. it mentioned some of the drug-bug combination that will be put under surveillance in ...
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169 views

Do archaea undergo the same horizontal gene transfer processes as bacteria?

Do archaea also undergo processes like conjugation, trasformation, etc.? If not, do they have their own horizontal gene transfer methods? Can bacteria conjugate with archaea?
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Where do i find microbes like tardigrades in winter

Just bought a microscope and i want to see microbes like tardigrades. Where should i look? I have found many bacteria and what seems to be motionless single celled organism. I want to see movement ...
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105 views

How many strains of influenza can occupy a person at one time? [closed]

Could a person's immune system be built eventually to be able to inject all the known strains with in to stay immune?
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2answers
143 views

What culture medium should we use for tap/drinking water bacteria?

We are a group of high school students from Manila who is performing an investigatory project on a topic that we chose which, at first glance, seemed simple, but turned out a bit complicated. We're ...
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What would possible dangers really be from encountering alien microbiology?

From what I understand, our biological "enemies" on Earth are specifically designed to target us due to an "evolutionary arms race." With a few exceptions like aids, bird flu, mad cow and diseases ...
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104 views

How do I look for pathogen in water with a microscope? Is it possible?

I am a 9th grade student from Manila and we're currently working on an investigatory project. We want to know how effective SODIS or solar water disinfection is. The purification method claims to be ...
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2answers
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Estimation of the amount of bacteria

For my first research project in microbiology I've chosen to determine the effectiveness of sanitizers and soaps in killing bacteria from our hands, however, after putting the bacteria on agar plates ...
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1answer
41 views

Recommended reading to quickly get up to speed on infectious diseases

I am in the beginning stages of a capstone project for my master's in Data Science. For various reasons, I have landed on the subject of Clostridium difficile infection. My background is in Computer ...
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Has autogamy been observed in any animals?

By my understanding, reproduction that is sexual, but with both gametes contributed by the same individual, is not at all uncommon in the plant kingdom. But where, if anywhere, does it occur in the ...