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Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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What defines a microbial species?

I know that microbes are not capable of sexual reproduction, thus sorting them into species according to "groups that can interbreed and generate fertile offspring" should not apply.
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1answer
23 views

Do Tardigrades preserve water or replace water?

If you find something funny in my argument, then please pardon me, as I lack knowledge of biology. I was reading an article titled as "Water Bears Can Replace All The Fluid In their Bodies With A ...
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42 views

What is the most readily usable form of nutrients/energy source for heterotrophic microorganism in general?

Consider a nutritious mixture containing abundant soluble sugars, soluble lipids and soluble protein etc. Would heterotrophic microbes preferentially use sugars first > lipids > proteins > insoluble ...
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1answer
73 views

Spores are Seeds

I think that the spores produced by the bread mould plant are actually its seeds but my textbook says that it isn't. I think that spores are seeds of bread mould because both spores and seeds perform ...
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1answer
203 views

What really causes the toxicity of wrongly-thawed meat?

(Note: I've seen multiple related questions over at Cooking.SE, but I'm looking for a scientific explanation, not cooking advice!) I've been researching this for a couple of hours but the more I read,...
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1answer
33 views

Proteins that cannot form biofilm?

I am trying to build a machine learning training set for bacterial protein sequences that form biofilm, and that cannot. I collected the positive sequences from the GO ontology website but for ...
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1answer
136 views

A simple question about amoeba (Naegleria fowleri)

I have a very simple question about Naegleria fowleri amoeba. Some experts claim that boiling the water for 15 minutes kills Naegleria. However, I have studied somewhere that Naegleria can turn into ...
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1answer
33 views

Feeding behavior of organisms that prey on microorganisms

Is there a specific term for organisms that feed on micro-organisms?
4
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1answer
143 views

How is Coenzyme A Transported to the Matrix?

So, I've been researching cellular respiration on my own, and trying to keep track of most of the major processes. However, I do have one question left: I can't seem to find any sort of information ...
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2answers
770 views

Is working with (nitrile) gloves around a bunsen burner safe?

Recently I have started in a new microbiology lab, and with a new lab come new habits. When I was working with my bacterial (liquid) culture next to the flame (Bunsen burner) wearing nitrile gloves, ...
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2answers
59 views

Which life is supported in sterilized 100% pure water? [closed]

As sterilized 100% pure water does not contain any mineral and ion, so which type life can survive in sterilized 100% pure water?
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0answers
74 views

MOPS Media Recipe Protocol - Well Defined

I am trying to find a clear protocol for making MOPS media. However, when looking online, I am finding that this media is loosely defined and I can not find a good protocol. (I have been looking for a ...
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0answers
25 views

How would you find and cultivate specific forms of algae?

Knowing how simple and efficient it is to grow algae, I started wondering how a person would specifically isolate and grow different strands of algae. Say I walk to the closest body of water and took ...
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2answers
98 views

What is a Slurry in Microbiology?

I have been reading this article and they refer to this thing called 'slurries' that can be made from soil samples. This is in the context of being some sort of microbiological method. "slurries can ...
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0answers
41 views

Exclusion Limit and permeability of outer membrane

According to this textbook: P aeruginosa, for example, which is extremely resistant to antibacterial agents, the outer membrane is 100 times less permeable than that of E coli.[pg.no 27] and then ...
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2answers
101 views

Can any organism possibly have the capability to create a new species every time it reproduces?

There are lots of different kinds of living organism in our planet. So i was wondering if such an organism could possibly exist? Can any organism possibly exist, that would have the capability to ...
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1answer
81 views

Serial dilutions

A student wants to make serial dilution of a sample. She starts with a 1mg/ml sample and 10 tubes, (labelled 1-10) each containing 2 ml buffer. The student puts 1ml of sample solution into tube 1 ...
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0answers
22 views

Does Antioxidant prevent oxidation that creates free radical?

I have already known that antioxidant can neutralize oxidant/free-radical, but i want to know if antioxidant can also prevent the oxidation or not. I want to know to how it works if antioxidant can ...
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4answers
640 views

How much vitamin C has a noticeable effect on E.coli k12

I am doing a research on effects of different concentrations of vitamin C on serial dilutions of E.coli k12. I am looking for literature that has already shown effects of vitamin C on E.coli, but ...
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1answer
52 views

How are bioremediation microbes removed from the environment once they perform their function?

In the case of bioaugmentation, microbes are introduced into the environment to clean up and absorb a certain pollutant. My question is how is that microbe then removed from the environment once it ...
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2answers
347 views

What are the (correct) terms for these flagella-arrangements?

I came across the following diagrams depicting two kinds of arrangement of flagella over cells. Now according to my (very unreliable) school textbook, the arrangements are termed as: A - ...
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1answer
762 views

Why cornstarch for athlete's feet?

It seems like a lot of foot (anti-odor or antifungal) powders are often based off of cornstarch (i.e., as the primary filler). But wouldn't cornstarch (which, in some places, is also a common food ...
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0answers
66 views

About which cells in the human body do we know most? [closed]

Are there model cells (in the sense of model organisms) in the human body (other than E.coli) about which is known most (with respect to structure, function, metabolism, gene expression, ...) and to ...
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1answer
1k views

Picture of a camel's red blood cells

I'm an art student in need of a little help. I'm looking for microscopic pictures of camel's red blood cells, I need their shape for an art project. The pictures I find on Google seem a little ...
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1answer
75 views

S.pyogenes binary fission rate/speed

For my Math IA (IB SL) I want to calculate the average amount of time that one viable S.pyogenes bacteria will need to create an ID50. I cannot find the average speed for the divison of any ...
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2answers
57 views

Hand-washing resistant bacteria. Will they evolve one day?

I know that soap kills bacteria by dissolving their membrane. But it is not 100% effective. A small portion of bacteria which survive replicates and I have to wash my hands again. Will this cycle ...
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1answer
142 views

Spores bacillus subtilis in milk

I need to inoculate spores into milk and then count on the plate. I never see a spore, so how i recognize it from the germ cell? does anyone know the procedure and if there are differences from a ...
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49 views

presenting log MPN results

I'm reviewing literature regarding effectiveness of wastewater treatment techniques. When considering faecal coliforms, concentrations are generally presented in terms of most probable number per ...
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1answer
35 views

What if we blend hydras to form a soup. will they still regenerate to form new individuals? [closed]

Hydras can regenerate if cut into pieces. But if we take a pound of alive hydras in a blender/grinder and blend/grind them to form a jelly like liquid made of hydras where we have finely blended them, ...
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0answers
40 views

What biproducts do vinegar eels produce?

What chemicals, acids, gases or other biproducts do vinegar eels (Turbatrix aceti) produce (in raw vinegar and/or in the vinegar-creation process, which process may have them in environments including ...
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1answer
137 views

Inoculating agar plates with microbes suspended in sterilised water?

We were having a problem with our experiment (culturing microbes on homemade nutri agar) so we decided to change our set-up. It already cost us quite some money. So, we saw this procedure. Can anyone ...
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0answers
37 views

Can virus be used to kill bacteria? [duplicate]

You are watching TV discussing viruses called bacteriophage that kill bacteria. Your roommate says wow, may be virus can be used to kill bacteria that infect people. Is this possible?
4
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1answer
3k views

Cyanobacteria: Gram negative or Gram positive?

My (school) textbook states that cyanobacteria ("blue-green algae") are Gram positive (no references provided). They mention this at several points over the chapter we're doing so it doesn't look like ...
4
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1answer
71 views

How likely will 2 normal bacteria “attack” each other if they infect the same parts of your body?

I read this interesting article online about how deadly bacteria are known to attack other deadly strains of bacteria. https://www.google.com.sg/amp/s/phys.org/news/2013-02-deadly-bacteria-remarkable-...
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3answers
262 views

Could plasmids and conjugation mechanisms be used against antibiotic-resistant bacteria? [closed]

I'm surprised no one has mentioned something like this. Plasmids are often exchanged between bacteria, sometimes through conjugation. In particular, conjugation could be considered an "open-port" ...
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2answers
465 views

Do micro-organisms survive and live normally in a zero-g environment?

Have there been any experiments in space regarding micro-organism behavior? Are they able to move around in a drop of water like they do on Earth? Are they able to eat and reproduce normally? Edit: ...
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3answers
221 views

Kitchen sponge microbiomes; management versus replacement versus “don't use sponges”? [closed]

I have unfortunately just skimmed through the recent open access paper Microbiome analysis and confocal microscopy of used kitchen sponges reveal massive colonization by Acinetobacter, Moraxella and ...
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0answers
33 views

Bioenergetics of bioconversions: is there a surplus of energy?

By bioconversion, I mean a microbial process during which resting cells are used in order to convert a substrate to a desired product, whithout cell growth. For example, in this paper, they used ...
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2answers
687 views

If you measure the O.D. of cells at one wavelength, is it possible to find its equivalent O.D. at another wavelength?

For example, I measured the optical density (O.D.) of some cells at wavelength 550 nm but I should have used 600 nm. Is there a way of converting my O.D. to its equivalent in another wavelength?
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1answer
80 views

How long can Vibrio cholerae live in its ambiental optimal context? [closed]

How long can the Vibrio cholerae live in its ambiental optimal context? Thank you in advance. My problem is that i can't find this information. I did some research on google, but nothing was ...
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0answers
197 views

What are chromosome dimers and how do they relate to recombination?

I am reading the book Molecular Genetics of Bacteria 4th ed. and I don't fully understand the concept/visual representation of dimer chromosomes. Consider the following quote (pg 35): an odd number ...
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1answer
38 views

Clinical Microbiology Books [closed]

I am looking for a good book about Clinical Microbiology, particularly good in Bacteriology and Bacteria Diagnosis (medium, plates, type of colonies...). With good photos. What are the bests in your ...
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0answers
153 views

Overnight bacterial culture [closed]

I am working now to prepare an overnight bacterial culture. My question is should i pick only one colony of the bacterium streaked plate in order to make an overnight culture and what will happen if i ...
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2answers
194 views

Biology experiment with algae method help

I'm planning a biology experiment I need to complete. I was planning on measuring the affect of pH on algae growth. I chose chlorella to be the test algae I use (are there any algae that would be ...
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1answer
2k views

what is the reason for using 70% alcohol (ethanol)l? [duplicate]

For the purpose of sterilization I am using 70% alcohol (ethanol) for a really very long time. but whenever I ask about why actually we are using 70% alcohol till now I did not get a very specific ...
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2answers
219 views

Microorganisms in Louis Pasteur's experiment of beans

Louis Pasteur's experiment proves that microorganisms do not grow from non-living matter, he discovered that microbes can cause food spoilage. So, where do microorganisms come from? Do they come from ...
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0answers
85 views

small pellet size after protein production in E. coli

I expressed a protein using 0.5 mM IPTG in E. coli (BL21 cell line straight from a glycerol stock was used as starter culture, ampicillin as antibiotic at 100 mg/L) but the cell pellets were very ...
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1answer
1k views

Count colonies in the right way

I use Rose bengal agar cloramfenicol for search yeast and molds for inclusion in petri plat. Which is the method correct for count? I'm not sure if i count everything or only the bigger one?
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If I need to view 0.3 UM micrometers what X strength microscope do I need ? (Streptoccocus Mutans)

Would also be glad if you had more charters of bacterias/virus/parasite pictures and sizes. Also maybe if you have other microscope specification for viewing them clearly I am completely new to ...