Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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172 views

Are there any known microbes capable of living in almost pure carbon dioxide?

Some bioengineering is investigated in high carbon dioxide environments. Are there any microbes like bacteria or Archaea capable of living and multiplying in such environments?
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1answer
135 views

How are logarithmic trendline equations used to determine antibody concentrations in ELISA?

For example, say you have plotted a standard curve and managed to obtain a logarithimic trendline equation like: y = mln(x) - b And you are working with data that includes: a) standards,their ...
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Microorganism ID?

I found this microorganism in a wet moss sample. It was very active and moved around quite quickly, and it looked to be using cilia to move. It had no coloration. I got some pictures at 400x: Any ...
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Disposing biohazardous material

I had conducted some disc diffusion tests with E.coli and S.epidermidis. Now, I wanna dispose these petri dishes and was wondering the correct way to do it. Many websites mention adding bleach to the ...
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1answer
24 views

ID microbe found in moss sample (USA)

I found this microbe after putting a wet moss sample under my microscope. It latched on to a piece of moss and scanned the area around it for prey, and when it didn't find any, it slowly swam away. It'...
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1answer
238 views

“Antibiotic resistance” equivalent in archaea for selection during cloning

I'm beginning to work with halophilic archaea and I'm trying to figure out a good way to select for cells that have taken up a plasmid. Obviously, one can't use antibiotic resistance since they are ...
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1answer
69 views

Why does open olive oil keep so long?

This is a question about the biology of microorganisms, not about food safety. While most of our foods spoil quite quickly (it is recommended to throw food away after 2h in the danger zone), for some ...
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Methods for getting clean C. difficile spore preps without spores clumping together

I'm a microbiologist recently who recently started working on an ongoing C. difficile project for the first time. One of the things I need to do is verify the lower detection limit of my qPCR ...
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Does Tryptic Soy Agar (TSA) contain L-cysteine? (Question about Legionella)

I ask because Legionella sp. requires the presence of cysteine. If TSA does contain L-cysteine, is it sufficient to grow Legionella?
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Do E. coli change color (from white/grey to yellow) when they grow older?

In my experiment, I had white colonies initially and they turned yellow. So I thought it´s natural that mature E. coli colonies are yellow and young are white. But it could be that I had two types of ...
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1answer
39 views

Examining antibody - target interactions in PyMOL

I'm a student currently looking at antibody responses against a viral target protein of interest. I have my own, annotated PyMOL session of my protein and I also have .pdbs of crystallised antibody ...
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1answer
62 views

Isolating colonies in a pure culture

I want to conduct antimicrobial susceptibility tests for which I've to culture bacteria first. Since I've bought KWIK-STIKs, (KWIK-STIK contains a single lyophilized microorganism which have to be ...
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1answer
47 views

Does SARS-COV-2 produce toxins?

I wonder whether toxins are produced only by bacteria and fungi and not by viruses. Looking at the paper below, I read that several toxic proteins are produced by SARS-COV-2. Can these proteins be ...
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60 views

What material is permeable for plant roots, but “impermeable” for fungi & bacteria?

I'm designing an experiment where the roots of the plant will grow in a sterile environment while the shoot will grow in a non-sterile grow room. For this reason the environments of the shoot and the ...
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1answer
54 views

Salt Bridge for Microbial Fuel Cell: Full or Partly Full?

I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (...
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1answer
71 views

How to convert system of equations with explicit substrate consumption into an implicit one?

I have a description of competition between two bacteria that is described by the system of equations: S = substrate, μ = bacterial division rate, K = half saturation constant, y = yield (cells per ...
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48 views

Does the Bacteria culturing medium effect its performance?

Different mediums are used to culture different bacteria species in a Lab environment. I noticed that there is not one optimal medium for every bacteria. I am more curious about culturing ...
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1answer
110 views

What is the relationship between a “main” strain genome and its variant genome in archaea?

I am going to analyze DNA sequencing data in order to try to extrapolate some information about the survival strategy of Pyrococcus Furiosus to gamma irradiation, as maybe you already know from all ...
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1answer
39 views

What is targeted gene disruption?

I am a bit confused about what targeted gene disruption means. I was reading this article in which they compare the Pyrococcus Furiosus genome with a genetically tractable strain P. Furiosus variant ...
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How do scientists create an environment that favors the selection of a microbe with higher production of a certain enzyme?

Let's say you want to create a strain of bacteria that is resistant to heat. You expose all bacteria to high heat and those who have mutated to survive this heat will be the survivors. Then you allow ...
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1answer
498 views

This graph about logarithmic and arithmetic plots. Shoudn't it be the other way around?

From the textbook 'Brock biology of microorganisms, 15th edition' The black lines points to the linear graph with the caption 'Logarithmic' and to the curved graph with the caption 'Arithmetic'. Isn't ...
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How do fungal hyphae dig into substrates (such as grains)?

When a fungus needs to expand its nutrient-acquiring network of hyphae, it can dig into, say, a grain of rice. How does it drill openings into the grain?
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What is the purpose of putting multiple blanks on a microplate reader? [closed]

I am new to biology and I saw that in the software that controls the microplate reader several targets are set, what is the purpose of this?
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40 views

How can I learn botany/plant ID by myself?

I am interested in sources and methods regarding botany and plant ID, and being a self-taught person. Textbooks, sites etc. Thank you all in advance!
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How to express total eradication of bacteria (inhibition zone) when placed into a table

I am a grade 9 student studying the inhibition zones of several different essential oils by comparison to Tetracycline to prove there is merit to treatment of bacterial infection (investigation is ...
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1answer
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Is the stuffy environment due to using masks all day, helping in spread corona virus in people?

I came across a research that stated, the most disease ridden place in a toilet is actually the air dryer. The hot and moist microcosm that develops around the dryer due to frequent use helps in ...
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107 views

Does one wing of fly contain harmful microbes and the other contain only antibodies?

There was this study but the conclusion and it's source's relability seemed a bit off. How come a house flies one wing contain all microbe and the other don't.
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In what stage of bacterial growth can penicillin inhibit the growth of the cellular wall?

The bacterial growth has 3 main stages: lag-phase, log-phase and stationary phase. I was wondering in which one of them penicillin can inhibit the growth of the cellular wall of bacteria and why. I ...
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1answer
50 views

What factors affect the rate of siderophore production by microbes?

Microbes produce siderophores which are chelators and bind to iron. This is how microbes absorb iron from soil or rock. What causes microbes to increase or decrease the rate of production of ...
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Can the metatranscriptomics replace the approach of functional metatranscriptomics/functional metagenomics?

While metatranscriptomics reveals information about the expression of genes and their functions too, Functional metatranscriptomics (https://www.nature.com/articles/ismej201167) allows the ...
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1answer
124 views

Can toilet or sewage aerosol transfer eggs of human parasites?

Flushing a toilet produces hundreds of thousands of tiny droplets containing viruses and bacteria (source). Additionally vomit and feces of infected people sometimes contain extremely high ...
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1answer
29 views

Nutrient agar as the medium for antibiotic susceptibility testing

I couldn't get Mueller Hinton agar to conduct antibiotic susceptibility testing. I'm thinking of just choosing nutrient agar as the growth medium for the bacteria Staphylococcus epidermidis. Will ...
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1answer
50 views

Are there any organisms that perform photosynthesis-like processes with thermal energy alone?

The closest I can find are thermophiles, but it seems they use the chemicals present in the hot environment for energy, not the heat itself.
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What does T and the numbers mean in T coliphages?

I have searched a number of websites and articles relating to nomenclatures or phages but never found anything explaining what T in T-4,5,6.. Phages mean in the Coliphages. Also, what does the number ...
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1answer
35 views

What disease / parasite / fungi has affected the mango in the picture?

The mango is found to have some kind of fungi / worm on it's surface . This has affected a huge number of mangoes in the same tree . It can be described as white and fibrous / weblike structure near ...
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Soil microbiology reference

I am after some good reference on Soil Microbe Ecology and Biology. I am a biophysicist, and have read the very gentle approach of "Life in the Soil" (James Nardi). Another book I have been ...
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2answers
72 views

How do microbes spread from a small place to a very large place?

Bacteria are very small, so they cannot move very far on their own. Viruses cannot move themselves at all. So how does a specific type of bacteria or virus spread from a small place to a very large ...
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873 views

Has autogamy been observed in any animals?

By my understanding, autogamy, i.e., reproduction that is sexual, but with both gametes contributed by the same individual, is not at all uncommon in the plant kingdom. Does this also occur anywhere ...
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1answer
231 views

Inoculating agar plates with microbes suspended in sterilised water?

We were having a problem with our experiment (culturing microbes on homemade nutri agar) so we decided to change our set-up. It already cost us quite some money. So, we saw this procedure. Can anyone ...
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1answer
139 views

Alternative dyes for Gram staining

In the Gram stain, is there any replacement for primary stain, secondary stain, decolourizer and mordant? What results will the replacements produce? I found that crystal violet can be replaced with ...
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265 views

Is there a favoured mathematical model of bacterial growth?

One of the most regularly measured phenotypes in bacterial synthetic biology is growth. Anyone who does wet lab microbiology or synthetic biology will have performed a growth curve measurement. ...
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How do absorbance readings indicate diauxic growth of bacteria?

How is growth rate an indicator of carbohydrate use? How do absorbance readings from a spectrophotometer show growth of bacteria if the absorbance readings are taken several times over the course of a ...
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1answer
132 views

How long would you expect a designed plasmid to remain in a population of E. coli after transformation?

When transforming E. coli with a genetically-modified plasmid, how quickly/often is this plasmid "lost" because of mutation, or horizontal-transfer, or "dilution" over reproduction ...
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148 views

Why can't certain nucleoside analogs be used to label bacterial RNA?

A 2020 publication in Nucleic Acids Research 1 includes the following passage: A variety of nucleoside analogs have been developed for metabolic RNA labeling in various eukaryotic cells (9–16). Among ...
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Recommendations for a haemocytometer (phase-contrast)

I'm looking for recommendations for a haemocytometer that can be used with a phase-contrast microscope. I want to use it to quantify the vegetative and spore fraction in my samples. Can I use any ...
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Is publication an important criteria to get a post doc in microbial ecology?

I am a researcher in microbial ecology , currently searching for post doc in European countries. I have publication less than 1 impact factor. Will this hinder my chances of getting selected . Please ...
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Are single-celled organisms capable of learning?

I've read that the amoeba is capable of learning. Since these protists have no nervous system, it's safe to assume that even highly simplified learning mechanisms of the Aplysia are miles off in the ...
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Are viruses self-propelled?

So obviously, viruses are nonliving. But when my teacher was teaching viruses in the video (we're doing "flip" learning this semester), the way he described it, it seemed like the viruses responded to ...
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Concentration/molarity of lichen acids (particularly oxalic acid) in soil/rock substrates?

I am trying to design an experiment testing the efficacy of lichen acids to solubilize and mobilize nutrients from minerals in a lunar regolith simulant. My research so far indicates that oxalic acid ...

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