Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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Will obligate aerobic bacteria die if placed in a buffer solution even in the presence of oxygen?

The electron transport chain in aerobic bacteria functions by pumping H+ out of the cell to establish a concentration gradient. So if the bacteria are placed in a buffer solution having pH equal to ...
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What is the difference between chrysophytes and protozoa?

Amoeba is present both as a protozoa and as a chrysophytes.How to understand ,with which defining feature to which group to classify into?
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How does temperature impact protein synthesis in E Coli? [closed]

A student wants to study the effect of temperature on protein synthesis in E. Coli. How would you design an experiment to test this out and what results would you expect?
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If a human takes antibiotics are all bacteria in the body killed?

From my basic understanding, antibiotics kill living things, bacteria for example. Do the antibiotics consumed by a human-being distinguish between what they kill? Or do they just kill every bacteria ...
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Is there any description about how microbes spread from a small place to a very large place?

Is there any materials (such as books, papers or others) about how microbes (such as the bacteria or virus) or a special kind of microbe spread from a small place to a very large area (such as the ...
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Is there such a thing as an autotroph that doesn't use photosynthesis?

I've heard of photoautotrophs, which are just autotrophs that use photosynthesis. What I'm wondering is if there's any other way for an organism to make its own food that doesn't involve it using ...
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Quantification of various amino acids from bacteria?

I would like to characterise how much of various (uncommon) cytosolic amino acids are produced in bacteria, and was wondering if there are good suggestions of how to go about doing this. I know that ...
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Does thimerosal have some of the same mechanism of action as sodium benzoate?

I noticed that thimerosal's molecular structure is basically just sodium benzoate with an attached chain that includes a mercury atom. Sodium Benzoate Thimerosal The Wikipedia article for thimerosal ...
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How can I obtain a uniform bacterial sample?

I'm currently doing a research project wherein I will be testing the antibiotic resistance of a bacterial sample. Due to a risk of contamination during transport, I'm not allowed to request a uniform ...
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How does the DNA cross through bacterial cell wall during electroporation?

There exists a lot of literature on electroporation of Gram-positive and negative bacteria. Most of it gives an explanation that electroporation works by creating transient pores in cell membranes of ...
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Immunity to one's own microbiome

It seems that a human bite can be very dangerous, because of the myriads of bacterial species found in saliva. This leads to several questions that, perhaps, may have the same single answer. But, I'...
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Salt Bridge for Microbial Fuel Cell: Full or Partly Full?

I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (...
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How to choose a plasmid used for replenishment after gene knockout?

Is it based on strain selection or knockout gene selection, or both?
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Why Vibrio Cholera produce its toxins?

I was wondering, why V. Cholera synthesize toxins which induce in humans diarrhea? I know that diarrhea is protective mechanism for humans, to get rid of bacteria. But why V. Cholera just stop ...
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Why does an autoclave need to pressurize steam?

I've looked this up on other sites and they say that an autoclave needs high pressure in order to raise the boiling point of water so that you can achieve steam of a higher temperature. If you look ...
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Human Digestion of Cellulose?

Most animals can digest the cellulose in grass because of the anaerobic bacteria called Fibrobacter succinogenes living in their rumen (gut). The bacteria produces the enzyme cellulase and is ...
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Examining antibody - target interactions in PyMOL

I'm a student currently looking at antibody responses against a viral target protein of interest. I have my own, annotated PyMOL session of my protein and I also have .pdbs of crystallised antibody ...
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Can human pathogens present on plants become plant pathogens?

Since human pathogens sometimes live on the surface of plant leaves(read here and and here), and there are plant-pathogen microbes also living on leaf surfaces, is it possible for plant virulence ...
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1answer
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How could pharmaceutical metabolizing bacteria be used to synthesize them?

Lets say I have a pharmaceutical "P". It can be broken down by enzymes of one strain of bacteria into two molecules one of which can be further metabolized enzymatically by a different strain of ...
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What's this parasite?

Any microbiologists or parasitologists recognize this? It's from my pet bearded dragon in the eastern US and the vet's never seen it before.
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A question about microbes growing media

I was told in my microbiology course that blood agar can be put in GasPak system (anearobic) to grow strict anaerobes, but why would someone use blood-dependent bacteria in this experiment as these ...
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Why viruses “die” outside the host? [duplicate]

Why viruses "die" outside the host after sufficient time? I want to know the biological process behind it. What role being exposed to air plays in virus destruction? What role sunlight plays in the ...
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How effective is rinsing in removing microbes from surfaces

Microbes including viruses stay alive on surfaces depending upon the type of the surface like steel, wood, plastic, glass, marble etc. How effective is rinsing these surfaces with tap water in ...
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1answer
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What fraction of human cells gets infected during a viral infection?

As I understand, if a cell gets infected during a viral infection then it eventually dies. If an individual does not die of an infection, the percentage of cells that gets infected in the course of ...
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When purifying plasmid DNA after cloning, do you get endogenous plasmid in addition to your vector of interest?

Since bacteria naturally contain plasmids, when you do a cloning experiment and purify the plasmids at the end, do you get plasmid naturally present in that bacteria in addition to your vector? The ...
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Can DNA rings, i.e. plasmids, form as Möbius strips?

I know that plasmids can be present in a coiled form, which keeps the DNA strands together when they degenerate, by forming catenases. I was wondering, however, whether it has been documented to ...
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Can I use a Flask Weight Ring to increase RPM in an orbital shaking incubator?

I am working on a device that goes on top of the flask. This reduces the maximum RPM that I can use. Therefore I am wondering if I can use a Flask Weight Ring for Erlemeyer flasks in a orbital ...
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Freezing my dry encapsulated probiotics

Will my dry probiotics in capsules die if I freeze them for a long period of time?, even over a year. I'm talking about the regular capsules of probiotics that are purchased in little bottles to take ...
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Can/have bacteria been engineered to express ACE 2 receptors?

In light of SARS-COV-2 binding to the ACE 2 protein in human cells, I was wondering if any bacteria exist or could exist that possess this membrane protein. If not, do you believe that an ACE 2 like ...
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How can Saccharomyces cerevisiae live in a dried state for long periods of time?

I want to know how can the Saccharomyces can live for so long in dried form. I am talking about the dried yeast used in baking .
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How is the appropriately-stratified gut microbiome acquired in organisms performing horizontal transmission?

I am studying horizontal transmission of primary symbionts in insect reproduction. This reminded me of an earlier question I had asked, in which I learned that humans analogously acquired their ...
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What factors affect the rate of siderophore production by microbes?

Microbes produce siderophores which are chelators and bind to iron. This is how microbes absorb iron from soil or rock. What causes microbes to increase or decrease the rate of production of ...
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Humic Acid and PCR

Quite a few papers claim humic acid is an inhibitor of PCR reactions. I understand this is true when working with soil microbes, but how does it qualify to be a PCR inhibitor in general (i.e when not ...
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What is a general timeline to replace stainless steel SS 316 pipes in food industry?

Does the dairy and/or food industry (specifically but not limited to the USA)considers to replace their stainless steel SS 316 or SS 304 pipes after say 30 years or so? Could someone please share ...
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How does lactic acid cause muscle twitching?

It is well-known that lactic acid buildup (often caused by workouts) causes muscle twitching. Does anyone know HOW lactic acid achieves that effect?
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What is the principle behind Microagglutination test (MAT)?

Could some one please help me understand the principle behind the MAT. Also, is it only used to detect leptospira or can it be used to detect other pathogens as well? Kindly share links to papers if ...
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18 views

identificaation of the correct fragments with desired nucleotide sequences using probes

in making of rDNA, my text book has a point that is written above(the question). what i don't get is if we already know what the nucleotide sequence is why do we need to take out the gene having the ...
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Induce plasmid acquisition in bacteria to make them susceptible to antibiotics

Is it possible to use plasmids as a way of creating antibiotic susceptibility in bacteria. For example; in Microbiology, an introduction 13th edition by Pearson , Page 230 "the researchers traced the ...
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2answers
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Can single-cell organisms have through-holes? (Are donut-cells a thing?)

I've watched a YouTube video about microorganisms, which featured a shot (below) of a single-cell organism, that looked like it had several through-holes in its body. Somehow I have a pretty steady ...
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342 views

Serological assays measuring antibody response

Given that an appropriate immune response to a bacteria may be thwarted in an individual, including not producing all of the antibodies which are known to occur in people who have been infected, or ...
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773 views

What is meant by “complexity of carbon sources”?

My biology book states "life on earth depends upon carbon based molecules, most of these food sources are also carbon based. Depending upon the complexity of these carbon sources, different ...
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Can different Chromobactierum strains have completley different colony morphology?

In the analysis of an unknown bacterial broth I observed a gram negative rod genus. Upon plating I observed small, dark purple and convex colonies; corresponding presumably to Chromobactierum. After ...
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Can a bacterium express a virulence gene and not produce toxins and what may be the cause of this?

I am preparing a seminar on viable but non-culturable (VBNC) state in bacteria, mainly foodborne pathogens and I just saw this line in a journal article by Lothigius et al that "the expression of the ...
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What is the meaning of multicellularity?

I can't understand what multicellularity is. Wikipedia states that any organism having many cells is multicellular. By this definition bacteria can also be multicellular. For example, cyanobacteria ...
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How does the microbial environment in your gut initiate?

Clearly, a zygote does not harbor any microbes. As it develops, and the alimentary canal tissue is differentiated, I logically assume that there is still no microbial activity in the fetus's gut. I'm ...
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Why don't we have vaccination against all diseases caused by microbes?

People can be vaccinated against certain diseases. Principle of vaccination is to use live attenuated load or inactivated. My question is - why we dont have vaccination against all diseases which are ...
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Rhizosphere vs. Endorhiza?

In relation to microbiology and the naming of the various areas of the plant as it relates to microbial inhabitance, I am confused as to the difference between the terms endorhiza and rhizosphere. In ...
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132 views

Are there any known microbes capable of living in almost pure carbon dioxide?

Some bioengineering is investigated in high carbon dioxide environments. Are there any microbes like bacteria or Archaea capable of living and multiplying in such environments?
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Does turbidometeric method count cell number or cell mass?

Here it says that scattering is inversely proportional to cell number but also says that we can observe greater absorbance as cell number increases. So does turbidometeric method work on cell mass ...
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Identification of black growth on onion skin

I have encountered this black-colored growth on onion skins a lot. It seems to develop over the course of weeks and I have rarely, if ever, encountered the material on fresh ones. If I peel the onion ...

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