Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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What enzyme or bacteria could dissolve plastic?

What enzyme or bacteria could dissolve plastic aside from acetone? Preferably, low-cost and environmentally friendly. Any suggestions would be helpful. I'm targeting for thin plastic (low-density ...
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Are olfactive indicators always the result of inadequacy in sanitation?

Question If you can smell the scent of food in a thoroughly cleaned container, does that imply trace amounts of food particles remain? Scope It goes without saying that our sense of smell is ...
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Why do so many parasites infect definitive and intermediate hosts rather than just one host?

Many parasites infect multiple host species, with one host species being the definitive host (where the parasite reproduces), and the other host species being the intermediate host (where the parasite ...
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Is it unusual for populations of archaea to be found living inside trees?

The NPR News article and podcast Getting Fire From A Tree Without Burning The Wood begins with A scientist walks up to a cottonwood tree, sticks a hollow tube in the middle and then takes a lighter ...
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How protozoa spawns in newly formed ponds?

How big protozoa like Paramecium caudatum, Amoeba proteus, Euglena viridis etc. appear in newly created ponds of water? Suppose there was rain, and pond was formed. Or earth, dead leaves, live leaves ...
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Does a microwave oven disinfect food?

Imagine I am preparing food -- just about to put it into a microwave oven -- and some of it falls on the floor. Assuming it got some bacteria or other organisms (viruses?) on it, will the microwave ...
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Inoculation vs. vaccination

Is there any actual difference between inoculation and vaccination or are these terms interchangeable? In case the difference exists, would it be correct to say that inoculation is purposefully ...
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Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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Why are fungi and algae microbes?

Both fungi and algae have very small cells but so do most animals. Yet both of them are visible to naked eye just like other organisms. Why do we call them as microorganisms then ?
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How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?

I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
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DNA replication in $ E.coli $

$ E.coli $ has circular DNA which I guess implies one strand forms the outer circle and the other the inner one. So, is there a way to know if the replicated DNA forms the outer or inner circle? In ...
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What are the differences among food poisoning, gastroenetritis and diarrhoea?

I have now studied a major chunk of my microbiology course without understanding the basic difference between the three. The terminologies seem to be confusing. Is it a necessity for food poisoning to ...
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Could a strong magnet field repel the body before damaging it?

Could a magnetic field be strong enough to damage the body or erase the brain? Blood is slightly diamagnetic, so if the field was strong enough, could it repel a body evenly? Could a diamagnetic ...
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Can i grow bacteria on potato dextrose agar?

I was wondering if potato dextrose agar was a suitable environment for bacteria. Does it have all the requirements bacteria need or is it limited? Specifically: Escherichia coli, Staphylococcus aureus,...
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Relation between OD with different microbial growth [closed]

Equal volumes of cell suspensions of Escherichia coli, Saccharomyces cerevisiae, Streptococcus lactis and Mycoplasma pneumoniae, all have the same OD600nm = 0.50. Which cell suspension would have the ...
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In my DNA sequence for one sampling site there're W's; Y's; R's and K's?

I also just experienced the same thing with my DNA sequences, there are w's, Y', R', s' and k'. This is the very first time I've seen this. I am used to N but not these ones. Please kindly assist with ...
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when you are to non-culturally detect the presence of E. coli in the soil from a cow pasture?

You are to non-culturally detect the presence of E. coli in the soil from a cow pasture. You do this by extracting the total DNA from a sample of the soil and PCR amplifying with E. coli-specific ...
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227 views

Organisms in food deposits

I have just discovered that my kitchen sink pipe has been leaking for quite some time (possibly months) which has resulted in water and food deposits collecting in my cupboard. I smeared some of the ...
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Which is the reference 16S rRNA?

Recently, I've stumbled upon a fact, which hasn't bothered me for many years. The fact is that all universal 16S primers are written as "[FR][0-9]+" (in regex notation), that is they have a position ...
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For how long would a flu-contaminated parcel be an effective disease vector?

A parcel has been delivered and contaminated by a person who has the flu. For how long would the parcel be an effective disease vector?
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If a bacterium had a protein on its surface that humans also have, would it cause an autoimmune disease?

Suppose that a bacterium happened to have a protein on its surface. This protein can also be found in the human body. If this bacterium were to then infect a human with an otherwise normal immune ...
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Odds of finding superbug in soil?

There are tons of soil, rock, etc. being dug up, dug into, and moved all around the world each day. Paleontologists likewise regularly excavate fossils and both buried and frozen specimens, and ...
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Term of the type xxx-troph for a compound not used by an organism

A prototroph for compound X can make it A bradytroph grows faster if X is scavenged An auxotroph needs to scavenge X A hyperauxotroph lacks both the biosynthetic pathway and the transporters for X ...
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Storage of bacteria

Why are strains of bacteria stored when they are inactive (frozen)? What is the problem with storing growing cultures for long periods?
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Comparison between Transmission Electron Microscope (TEM) to Scanning Electron Microscope (SEM)

Can someone state the advantages of Transmission Electron Microscope (TEM) over Scanning Electron Microscope (SEM) and vice versa?
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1answer
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Is it possible to identify this microscopic particle?

So I was playing amateur scientist with my 11 year old. This photograph is from rainwater in a bird-feeding bowl in south England, at 300x. It looks biological, but I have no idea what this is. Is ...
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2k views

Count colonies in the right way

I use Rose bengal agar cloramfenicol for search yeast and molds for inclusion in petri plat. Which is the method correct for count? I'm not sure if i count everything or only the bigger one?
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What do the text annotations in an electron micrograph mean?

I saw a photograph taken from an electron microscope, with some text. I want to know what is the meaning of these numbers and letters? Thank you! Regards.
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Is there any kind of antibiotic effective against fungi?

I know that antibiotics usually have properties affecting specifically bacterial cells, like by inhibiting peptidoglycan synthesis. but do any antibiotics exist affecting eukaryotic cells, like yeast ...
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synthesis of zn nanoparticle

Sir the protocol I followed where it has been written... 1st using lactobacillus in Mrs media (50 ml in 250 ml conical)..we allowed to incubate for 24 hrs then added 0.1M znSO4 (50ml) and 0.4 M NaOH(...
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Why doesn't alum coagulate my cyanobacteria?

My collected rainwater has a pronounced blue-green tint which I believe to be due to the presence of cyanobacteria. I have been trying to remove the cyanobacteria using aluminium sulphate, but it ...
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82 views

Why are tardigrades so rare?

If tardigrades are so resilient and nearly immortal, why are they so rare when looking at samples of a pond or lake, where tardigrades should thrive? I've met people who've been studying protozoa and ...
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106 views

How can fresh cavities form at the margins of sliver amalgam fillings on teeth?

Silver amalgam fillings predominantly contain silver a known bactericidal agent and mercury which a known toxin and has bactericidal property. So how is it that the plaque bacteria survive near the ...
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For people who work in microbiology labs, what software do you use to manage your strains?

It's just a small microbiology lab that currently records everything on paper, and there's quite few mutants as well. Is Excel commonly used for this sort of thing? Or is there a better software to ...
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Growing bacterial culture on gelatin

I'm very new to experimental biology and don't know much about it. So, i was looking to grow some bacteria in petri dishes and looked up how to do it. Unfortunately, I'm finding it difficult to get my ...
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How is the right amount of nucleotides synthesised during homologous recombination?

I'm reading about homologous recombination in the context of double-strand break repair. It seems like the trick is that around the break, homologous recombination uses a template duplex to generate ...
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Why some eggs are bile stained?

Why eggs of some helminths( Diphyllobothrium latum, Trichuris trichiura, Ascaris lumbricoides etc) are stained with bile while eggs of other helminths( Hymenolepis nana, Enterobius vermicularis etc.) ...
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qPCR: Accounting for different sample mass when interpreting and comparing Ct

I'm trying to use qPCR to quantify the amount of a specific bacteria species in patient sputum, but I don't really have the option to use consistent quantities of sputum to elute DNA from. The qPCR ...
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271 views

How is Coenzyme A Transported to the Matrix?

So, I've been researching cellular respiration on my own, and trying to keep track of most of the major processes. However, I do have one question left: I can't seem to find any sort of information ...
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what is the characterization of mutant bacteria and how is it done? [closed]

I have a task to characterize protease producing bacterial mutants but I have failed to understand what it is and how it is done
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Volvox Cellular Reintegration

A couple of days ago, I answered this question over on worldbuilding.SE with some information about volvoxes, and commenter Liam Proven pointed out that one of the claims I made was dubious. I had ...
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Trying to find the right plasmid expression vector for e. coli c600

I am very new to the concept of gene cloning and transformation. I am trying transfer the arenite oxidase gene cluster (9kb in length) to E. coli strain C600. I want the E. coli strain to express this ...
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Is abiogenesis possible today?

Life on earth started about 3.5 billion years ago. I would assume abiogenesis happened because the conditions were right. Would the current earth conditions allow for new abiogenesis and completely ...
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Is it possible to see the microbes on your hand without using UV light or a microscope?

I know that black light (UV) is often used to see the microbes (colloquially referred to as germs) on people's hands but is there any way by which we can examine the microbes on people's hands without ...
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What did the evolution of multicellular animals look like?

What did the evolution of multicellular animals look like? Aspects of this question include: (1) Are there any living organisms that might be helpful in visualizing "transitional forms" between ...
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Why can't bacteriophages survive inside the human body thus negating the possibility of using them against bacteria?

I read about the therapeutic uses of bacteriophages in Pelczar's Microbiology. It was written that it is reasonable to think that bacteriophages could be used to kill pathogenic bacteria. However, the ...
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Infant immunization

I know that polio vaccine consists of small dose of polio virus itself, which activates body's immunity against the disease. An infant is given a no of vaccines including chickenpox, tetanus, ...
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Cells created using differently aligned proteins

I remember reading that scientist were making cells (I assume bacteria), that used differently oriented proteins to create a whole new class of life. Because apparently right and left aligned proteins ...
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Do unique communities of bacteria form inside water storage in plants?

I assume that plants, like animals, have an ability to fight off bacteria that invade their cells (support: here and here. However, water often harbors quite a bit of bacteria in even unsuspecting ...
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How Would I Isolate and Amplify a Viral Enzyme? [closed]

what procedures would I use to isolate and amplify integrase? If I am trying to study the integrase enzyme which is found in HIV how would I 1) destroy the viral capsule to release its contents. 2) ...

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