Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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259 views

Why is glycerol supplied/used as 87% solution?

While preparing glycerol stocks of my transformed bacterial cells (DH5alpha) the protocol I had to follow was that I would make one 50% glycerol stock (500µl culture, 500µl glycerol 50%) and another ...
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Are olfactive indicators always the result of inadequacy in sanitation?

Question If you can smell the scent of food in a thoroughly cleaned container, does that imply trace amounts of food particles remain? Scope It goes without saying that our sense of smell is ...
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Is it unusual for populations of archaea to be found living inside trees?

The NPR News article and podcast Getting Fire From A Tree Without Burning The Wood begins with A scientist walks up to a cottonwood tree, sticks a hollow tube in the middle and then takes a lighter ...
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How protozoa spawns in newly formed ponds?

How big protozoa like Paramecium caudatum, Amoeba proteus, Euglena viridis etc. appear in newly created ponds of water? Suppose there was rain, and pond was formed. Or earth, dead leaves, live leaves ...
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112 views

Can a pathogen be totally resistant to the human immune system?

Can a pathogenic organism be totally resistant to the human immune system? For the purpose of this question, the organism in question must cause a disease. Examples of organisms that would qualify as ...
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54 views

Salt Bridge for Microbial Fuel Cell: Full or Partly Full?

I'm making a microbial fuel cell out of benthic mud and salt water in containers. My salt bridge will be made of agar and table salt solidified in PVC pipes. Does the salt bridge connecting the anode (...
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84 views

How could pharmaceutical metabolizing bacteria be used to synthesize them?

Lets say I have a pharmaceutical "P". It can be broken down by enzymes of one strain of bacteria into two molecules one of which can be further metabolized enzymatically by a different strain of ...
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1answer
58 views

Can you increase the microbiota in an insect?

I know you can suppress the microbiota of an insect with antibiotics, but I´m am looking for a way to increase it. For example, can I feed an insect with something that helps boost the bacteria like ...
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How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?

I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
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DNA replication in $ E.coli $

$ E.coli $ has circular DNA which I guess implies one strand forms the outer circle and the other the inner one. So, is there a way to know if the replicated DNA forms the outer or inner circle? In ...
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Why do bulls-eye rashes look like they do?

People infected with Lyme Disease often present with an erythema migrans ("migrating redness") rash. Most often, these rashes are in the shape of a bulls-eye. Rash image. Presumably, this is a ...
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51 views

How is Pseudomonas aeruginosa diagnosed?

I know that P. aeruginosa is cultured on an agar plate, but which media or assays make it distinct from other Gram-negative bacteria?
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Could a strong magnet field repel the body before damaging it?

Could a magnetic field be strong enough to damage the body or erase the brain? Blood is slightly diamagnetic, so if the field was strong enough, could it repel a body evenly? Could a diamagnetic ...
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907 views

What concentration of hydrogen peroxide kills mold?

There are numerous articles online which claim that household 3% hydrogen peroxide can kill mold growing on household surfaces, yet I haven't managed to find scientific studies that show that. My ...
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35 views

In my DNA sequence for one sampling site there're W's; Y's; R's and K's?

I also just experienced the same thing with my DNA sequences, there are w's, Y', R', s' and k'. This is the very first time I've seen this. I am used to N but not these ones. Please kindly assist with ...
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when you are to non-culturally detect the presence of E. coli in the soil from a cow pasture?

You are to non-culturally detect the presence of E. coli in the soil from a cow pasture. You do this by extracting the total DNA from a sample of the soil and PCR amplifying with E. coli-specific ...
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100 views

If a bacterium had a protein on its surface that humans also have, would it cause an autoimmune disease?

Suppose that a bacterium happened to have a protein on its surface. This protein can also be found in the human body. If this bacterium were to then infect a human with an otherwise normal immune ...
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81 views

Is it possible to identify this microscopic particle?

So I was playing amateur scientist with my 11 year old. This photograph is from rainwater in a bird-feeding bowl in south England, at 300x. It looks biological, but I have no idea what this is. Is ...
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56 views

What do the text annotations in an electron micrograph mean?

I saw a photograph taken from an electron microscope, with some text. I want to know what is the meaning of these numbers and letters? Thank you! Regards.
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synthesis of zn nanoparticle

Sir the protocol I followed where it has been written... 1st using lactobacillus in Mrs media (50 ml in 250 ml conical)..we allowed to incubate for 24 hrs then added 0.1M znSO4 (50ml) and 0.4 M NaOH(...
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167 views

What makes an E.coli an E.coli, genotype or phenotype?

According to this paper, among 61 strains of E. coli they studied only 6% of the genes are common in all. Which means that the overwhelming majority of the genes are not shared. And wikipedia ...
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Why doesn't alum coagulate my cyanobacteria?

My collected rainwater has a pronounced blue-green tint which I believe to be due to the presence of cyanobacteria. I have been trying to remove the cyanobacteria using aluminium sulphate, but it ...
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85 views

Why are tardigrades so rare?

If tardigrades are so resilient and nearly immortal, why are they so rare when looking at samples of a pond or lake, where tardigrades should thrive? I've met people who've been studying protozoa and ...
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23 views

How is the right amount of nucleotides synthesised during homologous recombination?

I'm reading about homologous recombination in the context of double-strand break repair. It seems like the trick is that around the break, homologous recombination uses a template duplex to generate ...
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Why some eggs are bile stained?

Why eggs of some helminths( Diphyllobothrium latum, Trichuris trichiura, Ascaris lumbricoides etc) are stained with bile while eggs of other helminths( Hymenolepis nana, Enterobius vermicularis etc.) ...
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qPCR: Accounting for different sample mass when interpreting and comparing Ct

I'm trying to use qPCR to quantify the amount of a specific bacteria species in patient sputum, but I don't really have the option to use consistent quantities of sputum to elute DNA from. The qPCR ...
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what is the characterization of mutant bacteria and how is it done? [closed]

I have a task to characterize protease producing bacterial mutants but I have failed to understand what it is and how it is done
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Volvox Cellular Reintegration

A couple of days ago, I answered this question over on worldbuilding.SE with some information about volvoxes, and commenter Liam Proven pointed out that one of the claims I made was dubious. I had ...
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Trying to find the right plasmid expression vector for e. coli c600

I am very new to the concept of gene cloning and transformation. I am trying transfer the arenite oxidase gene cluster (9kb in length) to E. coli strain C600. I want the E. coli strain to express this ...
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165 views

Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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79 views

Is it possible to see the microbes on your hand without using UV light or a microscope?

I know that black light (UV) is often used to see the microbes (colloquially referred to as germs) on people's hands but is there any way by which we can examine the microbes on people's hands without ...
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1answer
208 views

Why can't bacteriophages survive inside the human body thus negating the possibility of using them against bacteria?

I read about the therapeutic uses of bacteriophages in Pelczar's Microbiology. It was written that it is reasonable to think that bacteriophages could be used to kill pathogenic bacteria. However, the ...
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Do unique communities of bacteria form inside water storage in plants?

I assume that plants, like animals, have an ability to fight off bacteria that invade their cells (support: here and here. However, water often harbors quite a bit of bacteria in even unsuspecting ...
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244 views

We know that the hepatitis C virus can live on surfaces for at least six weeks. Maybe longer. The infectivity study ended after just six weeks; why?

Background A paper has found that the hepatitis C virus (HCV) can remain infective for at least six weeks on ordinary household surfaces. You can see the free full text, or a summary for busy ...
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How to grow a spirulina biofilm on a carbon/graphite cylindrical cathode?

I am trying to grow a spirulina biofilm on my cathode for a fuel cell but it doesn't seem to be sticking to the cathode. I have a culture of spirulina and I've submerged a few cathodes in it to try ...
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36 views

A study found that the hepatitis C virus can live on surfaces for six weeks. Did they end the study before the seventh week began?

A study once found that the hepatitis C virus (HCV) can remain infective for six weeks on ordinary household surfaces. You can see the free full text, or a summary for busy clinicians. What happened ...
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What M9 stands for

Can anyone tell me what M9 stands for in M9 medium? I have tried googling but I still cannot find it.
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How does the intracellular accumulation of cyclic AMP cause edema in case of anthrax?

Edema factor is adenyl cyclase which is activated only inside the target cell. It leads to intracellular accumulation of cyclic AMP resulting in edema. How?
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E. Coli growth inhibited in Powdered Honey

I ran into problem with my method validation since the E. Coli that I've spiked into my Honey Powder sample can't grow in the media. The media I've used is Merck CCA (Chromogenic Coliform Agar). I've ...
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1answer
99 views

Why does tetracycline have such a broad spectrum of activity?

What are the structural and chemical characteristics that make tetracycline uniquely broad spectrum? I understand it acts on the A-site of the prokaryotic ribosome, but there exist many ribosome-...
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115 views

Can vaccines move from person to person?

Can someone who has been given a vaccine "infect" another person with the virus used in that vaccine? For example, let's say Bill is vaccinated against tuberculosis. If Frank is exposed to Bill, is ...
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115 views

Antigen molecular mimicry

Let us consider a situation in which the body is attacked by a microbe, and the microbe is captured by the immune system for recognition of surface antigens. The surface antigen recognized mimics one ...
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353 views

Glass petri dish to replace plastic petri dish?

I never use a glass petri dish before, but I just wonder if I can use it to replace for plastic petri dish because it can reusable. The only thing I worry is , in a Fridge's user manual, it said that ...
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How do I determine an acceptable $F_0$ value in sterilization?

Here's a sample question: The sterilization process using moist heat is used to kill B.stearothermophilus by heating to 115 degrees C and holding it for 30 mins. For B.stearthermophilus, it ...
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30 views

How Would I Isolate and Amplify a Viral Enzyme? [closed]

what procedures would I use to isolate and amplify integrase? If I am trying to study the integrase enzyme which is found in HIV how would I 1) destroy the viral capsule to release its contents. 2) ...
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How do we know that mild forms of rabies are nonexistent?

Wikipedia's rabies article says: "Death usually occurs 2 to 10 days after first symptoms. Survival is almost unknown once symptoms have presented, even with the administration of proper and intensive ...
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1answer
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Why is there such in interest in antibiotics against Pseudomonas?

I keep finding information on anti-pseudomonal antibiotics, or information specifying the antibiotic has anti-pseudomonal activity. Is there anything peculiar to this bacterium when compared with ...
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Virus plaque assay, pfu/ml calculation on plates with too little plaques formed

I am a student, so I hope it is okay to put this question here. I've done the virus plaque assay in a practical recently and have a few questions regarding the plaque count for pfu calculation. In ...
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Why do so many parasites infect definitive and intermediate hosts rather than just one host?

Many parasites infect multiple host species, with one host species being the definitive host (where the parasite reproduces), and the other host species being the intermediate host (where the parasite ...
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When two amoeba cells form from one amoeba, does spindle fibers form?

When two amoeba cells form from one amoeba, does spindle fibers form? Some sources say that spindle fibers do not form while an amoeba cell is undergoing cell division. If spindle formation does not ...

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