Questions tagged [microbiology]

Microbiology is the study of extremely small organisms. This includes organisms like bacteria, fungi, protozoa, and viruses.

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38 views

Forgot to cool slides before washing

I just finished an immunofluorescence experiment and I'm wondering what went wrong. The tissues seem dimmer than they should be. One mistake I made was: I completed the antigen retrieval step, in a ...
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90 views

Has Staphylococcus aureus ever been used against cancer?

Treating inoperable tumors with bacteria appears to be very effective, albeit being toxic to the patient as well. Has (live attenuated) Staphylococcus aureus ever been used against cancer? If the ...
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Red transcriptional reporter in Psudomonas putida

I need to build a transcriptional reporter in P. putida with high excitation wavelength,in order to decrease phototoxicity (I have been using mNeonGreen until now, and I would like to try something ...
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How do I “reinoculate” a petri dish with new bacteria?

Hypothetical question, intended to find a general procedure rather than case-specific recommendations: I have a petri dish full of growth medium with a large population of gram-negative, anaerobic ...
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How does the snail shells' fertilizer compare to regular fertilizers?

May I ask about the quality of the fertilizer derived from the shells and their effectiveness compared to other fertilizers on the market ?
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Bacteria surviving a β-lactam antibiotic

What changes can occur in the cell wall of a bacteria for it to survive a β-lactam antibiotic? I think that because a bacteria possesses peptidoglycan in its cell wall, they are β-lactam sensitive, ...
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32 views

How do microbes develop resistance to anitmicrobial peptides?

I would like to better understand how bacteria use the "strategy" of alternations to lipid A and membrane proteins in order to resist antimicrobial peptides of the immune system? It is my ...
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57 views

Of people who develop Alzheimer disease, are those people genetically predisposed to it?

I have read a lot lately about microbiological pathogens that are found in blood vessels in the brain of patient's with Alzheimer disease (positive association). So, I am confused whether there are ...
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47 views

How do cell repair mechanism ratios change as they age?

I have seen that embryonic stem cells are shown to use homologous repair for double strand breaks rather then non-homologous end joining (NHEJ). [1] I am wondering if something also happens to a cell'...
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32 views

Microbiology- Pseudomonas aeruginosa, BSAC data

I tested Pseudomonas aeruginosaagainst various antibiotics, using Stokes and Kirby-Bauer. When I compared my results with BSAC data it was completely different (understandable) but why for a lot of ...
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78 views

Values of Miu_max and Ks from parameter estimation?

Background I have this simple biomass growth model: $$ \mu = \mu_{max}\cdot \left(\frac{S}{K_S+S}\right) \cdot \left(\frac{1}{1+S/K_{iS}}\right) \cdot \left(\frac{K_{iP}}{K_{iP}+P}\right) \\ \frac{...
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Symbiosis of Pseudomonas Fluorescens

Have a modest background in molecular biology and presently considering a project that requires some knowledge of botany. Specifically, I seek details on the epiphytic relation between the microbe ...
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113 views

Enrichment of slow growing microbes: Large Test Tubes as an alternative?

Well, i would like to enrich bacterial and archaeal ammonia oxidisers from soil samples using Minimal Salt media. They are slow growing microbes. I would like to enrich them with the aim to produce ...
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122 views

Clumping factor test

I'm come across this question and I'm somewhat confused: In the test for staphylococci, the clumping factor detects: staphylothrombin DNase mannitol fermentation Fimbriae fibrinogen-binding protein ...
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24 views

What are the main principles of the Bacteriological Code?

Although the Bacteriological Code is an important guideline for taxonomists and microbiologists, describing the criteria for delineating species and higher taxa of the prokaryotes, I, and plenty of ...
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57 views

Alkaline Phosphatase

What is the predominant purpose of Alkaline Phosphatase in skeletal muscle fibers and liver cells. I know that it is a hydrolase enzyme that speeds up the degredation of proteins, lipids, starch and ...
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586 views

What is Mycoplasma Lyo medium?

Used for instance in this article The role of genital mycoplasmas as pathogens - - are generally neglected by diagnostic laboratories in the United Kingdom, possibly due to the lack of a ...
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108 views

Vitamin D oral intake, transportation and absorption

Several factors affecting vitamin D and its active form absorption and storage acidity of stomach (not significant effect) cytopathic effects of viruses cytopathic effects of bacteria where the last ...
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158 views

How are lophotrichous flagella of helicobacter pylori produced?

I am thinking the mechanism is something like first adhesins (mucinase). Howevever, this does not seem to be enough. How are the flagella of H. pylori produced?
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108 views

What are current abiogenesis hypotheses for first food source?

What is the very first abiogenetic piece of reproducing life (small piece of RNA + ribosome that randomly occurred?) hypothesized to have used as an energy source? I'd be interested in sources to what ...
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Bacterial 16S rRNA PCR amplification with universal primers

I am doing an experiment to amplify the 16S rRNA gene from bacteria present in gut contents of fish. After extracting DNA, I perform one-step PCR with universal bacterial primers (27F, 1492R) and I ...
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78 views

Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
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60 views

What are the characteristic structures of bacillus M. tuberculosis and what they cause?

I answered to this question: In most forms of the disease, the bacillus M. tuberculosis spreads slowly and widely in the lungs, causing the formation of hard nodules (tubercles) in the ...
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1answer
64 views

By what physiological mechanism do we not feel microbes (bacteria, etc.) living on our skin?

Background I know our bodies have a handful of ways to threshold our awareness of sensory stimuli: Neural density Sensory acuity I assume really tiny stimuli could fit between receptors e.g., ...
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Concentration/molarity of lichen acids (particularly oxalic acid) in soil/rock substrates?

I am trying to design an experiment testing the efficacy of lichen acids to solubilize and mobilize nutrients from minerals in a lunar regolith simulant. My research so far indicates that oxalic acid ...
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26 views

Need help designing an enzyme immunoassay (EIA)

I have a FITC-labelled DNA sequence and an anti-FITC antibody, however this antibody is not conjugated with HRP or any other enzyme. I work in a lab with limited finances, so is there a way I could ...
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22 views

How do tardigrades defend themselves from predators?

Tardigrades or water bears are well known for their ability to enter into a cryptobiotic state which allows them to survive extreme environmental conditions such as extreme temperatures or radiation. ...
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Is it possible for a prion to have a short incubation period ie 1 week or 1 month?

I know that all prions so far have long incubation periods but is there a possibility for one to have a short incubation period, is there any evidence that suggests this is possible. Thank you
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22 views

Creating a phylogenetic tree from my selected publicly-available sequences (WGS) in NCBI

I'm currently writing a paper on the comparison of virulence genes for a group of bacteria. I got my data from publicly-available whole genome sequences in NCBI. Now, I want to create a phylogenetic ...
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11 views

Eukaryotic cell lysate-based protein expression efficiency

What is average efficiency of eukaryotic cell lysate-based protein expression systems in terms of (mg of protein expressed) / (mg of lysate) ?
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24 views

Aerosolised particle released during autoclaving

During autoclave heat-up, the inside chamber's air is released in order to be displaced by steam. Because of that, many modern autoclaves are equipped with filters at the release outlet. The ...
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What is the difference between chrysophytes and protozoa?

Amoeba is present both as a protozoa and as a chrysophytes.How to understand ,with which defining feature to which group to classify into?
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1answer
47 views

Is there any description about how microbes spread from a small place to a very large place?

Is there any materials (such as books, papers or others) about how microbes (such as the bacteria or virus) or a special kind of microbe spread from a small place to a very large area (such as the ...
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57 views

Is there such a thing as an autotroph that doesn't use photosynthesis?

I've heard of photoautotrophs, which are just autotrophs that use photosynthesis. What I'm wondering is if there's any other way for an organism to make its own food that doesn't involve it using ...
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26 views

Can I use a Flask Weight Ring to increase RPM in an orbital shaking incubator?

I am working on a device that goes on top of the flask. This reduces the maximum RPM that I can use. Therefore I am wondering if I can use a Flask Weight Ring for Erlemeyer flasks in a orbital ...
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29 views

How can Saccharomyces cerevisiae live in a dried state for long periods of time?

I want to know how can the Saccharomyces can live for so long in dried form. I am talking about the dried yeast used in baking .
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How does lactic acid cause muscle twitching?

It is well-known that lactic acid buildup (often caused by workouts) causes muscle twitching. Does anyone know HOW lactic acid achieves that effect?
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19 views

identificaation of the correct fragments with desired nucleotide sequences using probes

in making of rDNA, my text book has a point that is written above(the question). what i don't get is if we already know what the nucleotide sequence is why do we need to take out the gene having the ...
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24 views

Induce plasmid acquisition in bacteria to make them susceptible to antibiotics

Is it possible to use plasmids as a way of creating antibiotic susceptibility in bacteria. For example; in Microbiology, an introduction 13th edition by Pearson , Page 230 "the researchers traced the ...
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41 views

What factors affect the rate of siderophore production by microbes?

Microbes produce siderophores which are chelators and bind to iron. This is how microbes absorb iron from soil or rock. What causes microbes to increase or decrease the rate of production of ...
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I need to identify the genus of this colony just by its morphology, can somebody help me?

it was grown on TSA it's a mesophilic bacteria. (it was contaminated by E. coli, so ignore the clearer and smoother colonies).
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How to convert AU/ml of bacteriocin into zone of inhibition in mm?

My questions is about measuring antimicrobial activity against bacteria on petri plate. Is it possible to convert or somehow get zone of inhibition in millimetre from AU/ml?
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21 views

sperm reaction of virus infected

we know when a human body cell infected by a virus , create interferon 1 to call other cells that surrounded it , but red blood cells couldn't do this because they loose their nuclear and other ...
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42 views

Virus Mortality Selection

This question is related to the recent Wuhan outbreak. Currently, mortality rate for the virus is somewhere around 2-3%. This is much lower than say, SARS (9.5%) and MERS (34.5%). As a thought ...
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Can microbes become “overweight”?

Just out of some weird thoughts, can a microbe (any single-celled organisms, accepting answers for both prokaryotes and eukaryotes) ingest too much food (e.g. via absorbing too much involuntarily ...
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Ideas for microbiology lab. Antimicrobial properties of cutting boards

I am an undergraduate student in Biology and I am trying to design a microbiology lab that will takes 3 weeks to carry out (1-2 day each week). I am going to examine the antimicrobial properties of ...
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29 views

Cleaning Masterclave

When i finish operation. My masterclave has smell, I think has media moulecular bind into the surface and microbial can live in. My labs don't have SOP to clean it. I promote use detergent but wonder ...
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How do I prepare a Basal medium for autotrophic mutant creation

Minimal medium (MM) was prepared by adding 2.0 g sodium nitrate (NaNO3) to 1 L of basal medium (BM) fol- lowing Correll et al. (1987). Chlorate resistant sectors (CRSs) were generated on two media i.e....
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Are most pathogenic bacteria facultative anaerobes?

I know that S. aureus and most gram-negative rods are facultative anaerobes. In terms of number of species, are most human pathogen-associated bacteria facultative anaerobes?