Questions tagged [microscopy]

The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

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Is the microscope that gives these images considered professional?

I bought a digital microscope from Dinolite, model AM73915MZT, The use of the microscope is for industries like textiles and ...
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2 votes
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How to prepare ethanol-preserved insects for imaging using SEM

I have aquatic insect larvae (soft-bodied) currently preserved in 70% ethanol. I would like to image these using a scanning electron microscope (SEM). I am wondering whether I need to/can start at ...
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Why do we have to scrape the inside of the cheek twice to get the sample of epithelial cells?

I was studying the theory of a practical exercise of preparing temporary slide of animal tissue from my laboratory manual. In the section about epithelial tissue, the first few steps of the procedure ...
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Distinguishing between yeast and bacteria

Can hematoxylin stain be used to tell apart yeast and bacteria cells Are there any other method to tell apart yeast and bacterial cells? I know hematoxylin stains histone protein but I am not sure how ...
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Why can SPRITE measure many simultaneous interactions?

Why is the SPRITE method not limited to a number of simultaneous interactions that can be measured, while microscopy and proximity ligation are limited to 2-3 interactions?
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Ventral View VS Dorsal View in cell experiments

I am trying to understand the difference between ventral and dorsal view of "cell spreading on a substrate" experiment in this article https://rupress.org/jcb/article/205/1/83/37623/A-...
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To which thing does "two-thirds" refer here?

Life and Letters of Charles Darwin, volume 2 includes the following letter from Darwin to Hooker, about a examining a plant that absorbs ammonia:- Reflect how little ammonia a plant can get growing ...
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How to take professional SEM images of bacteria?

I am new to using SEM microscope I would like to take clear pictures of Bacillus subtilis. My sample is dropped on a piece of paper and carbon-coated. The picture is showing crumbs of something (maybe ...
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Cannot seem to merge the two circles of a microscope (biological, binocular)

I have recently purchased a binocular microscope (Saxon Researcher Compact Biological Microscope 40x-1600x), and have had difficulty getting the two circles of light that I'm seeing through the ...
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What are the small white dots in an onion cell seen under an electron microscope?

We had to use an electron microscope in class for an assignment and used onion cells. On the 40x and 100x magnification there are tiny white dots and I was wondering if someone could tell me what they ...
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Why do bacteriophages look like tiny little robots? [closed]

I get it, evolution is funny and works in mysterious ways. But come on. You can't tell me this thing doesn't look like a nanobot. Why do bacteriophages look this way? With their cylindrical heads and ...
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Microorganism ID?

I found this microorganism in a wet moss sample. It was very active and moved around quite quickly, and it looked to be using cilia to move. It had no coloration. I got some pictures at 400x: Any ...
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ID microbe found in moss sample (USA)

I found this microbe after putting a wet moss sample under my microscope. It latched on to a piece of moss and scanned the area around it for prey, and when it didn't find any, it slowly swam away. It'...
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Reference for standardized histology rating criteria of microscope images

I am looking for a reference to a standard protocol for assessing the visual quality and diagnostic potential of a tissue sample that has been processed and stained for viewing under light microscope. ...
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Is this a real "double microscope" with two stages and two sets of objectives? Is it used in biology?

In this US late night TV broadcast1 a set has been created with some "science objects" for visual impact, and right in the center is an unusual looking optical microscope. The bottom looks ...
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colocalization by IFA?

I have done IFA (immunofluorescence assay) previously but i targeted only one protein or i tagged my endogenous protein with GFP/RFP then i performed IFA. I want to do the IFA in parental cell line (...
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How to select a microscope for deep microscopy? Specifically for Deep Learning/ML model development

I am new to biology and microscopy. I am a data scientist. I am faced with a question on selecting a good microscope that enables automation of scanning the slides. And cellular object detection using ...
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Can GFP reporting be used to track localization of peptides in the ER, Golgi, and plasma membrane?

Suppose I want to study the trafficking of a peptide throughout the ER, Golgi, and plasma membrane. An idea I had was labeling a secreted or plasma membrane integral protein with GFP and using time-...
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Recommendations for a haemocytometer (phase-contrast)

I'm looking for recommendations for a haemocytometer that can be used with a phase-contrast microscope. I want to use it to quantify the vegetative and spore fraction in my samples. Can I use any ...
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What does it mean to "cure" microscope slides?

I am reading a journal paper and I have come across the following statement in the materials and methods section regarding the preparation of microscope slides: Coverslips were washed once more with ...
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Why are cells in blood smears undetectable after absorbing moisture?

I'm a student learning how to prepare blood films and today I was curious about something, so I performed an experiment. After preparing a thin blood smear and drying it (no staining), I took a look ...
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Do electron microscopy change the structure of the specimen due to the vacuum step?

Since specimens have to be prepared using the vacuum, would the pressure change affect the structures of the specimen?
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What are these structures in blood from a nosebleed?

I decided to look at some blood from a nosebleed (not mine) under the microscope and took some pictures of the results. There many large orange structures interspersed within the blood cells and I'm ...
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Is there any reason to use an upright microscope over an inverted microscope?

I've never actually owned an inverted microscope, but it seems it has only advantages compared to an upright microscope: taller, heavier samples; no crashing the objective into the glass slide; easier ...
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How long do Ziehl-Neelsen stained samples keep?

I have acquired TB slides from my city's health center that are supposedly acid-fast bacteria (AFB) positive. However, when I took a look at it, I see basically no carbol fuchsin stains. They were ...
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Can anyone ID this microorganism?

I found this microorganism in a wet moss sample taken from my backyard in Richmond, Virginia (USA). I took some pictures of it at 40x, 100x, and 250x. I'm not entirely sure what it is, but I think it'...
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3 votes
1 answer
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Ferns: How big can a circular sorus be?

I looked at some small ferns under the microscope. The circular sori of the ferns I looked at were less than 2 mm in diameter. The microscope I used had rather weak magnification, so I didn't get to ...
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Is a FoldScope microscope suitable for plant cytogenetics?

I have recently been getting more interested in amateur botany, and have some background in genetics and genomics (PhD+). I have used standard light and confocal microscopes but don't have much ...
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umbrella term for microtiter plate, petri dish, cell culture flask etc

I'm looking for a term I can use in a software user interface that includes anything that may carry cell cultures or similar biological imaging samples (ie that may conceivably go into an automated ...
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Image Dataset for Cardiac cells for Mus musculus?

I'm working on developing a transfer learning model to detect cells in a culture using microscopic images. for that i require data set of images having microscopic images of various cell types. Is ...
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Transmitted electrons in TEM

When some electrons of the electron beam pass through the specimen, what exactly happens after that in order to produce the black white image? What I understand is that electrons transmitted collide ...
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How to obtain raphides from plants?

I recently found out that certain plants produce needle-shaped crystals called raphides. I am a physicist and I have an experiment in mind that could use these needles, but I would need to extract ...
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Is a wet-mount needed to use Immersion Oil?

When I prepare my slide to use immersion oil, do I really need to wet-mount the specimen? Can I do it with just the coverslip? I am preparing fungus spores. Using water for the wet-mount gives you ...
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Nuclear vs Cytoplasmic Fluorescence [closed]

When staining transciption factor proteins with fluorescent antibodies (Alexa, etc.), why are the fluorescence signals stronger in the nucleus as compared to fluorescence signals in the cytoplasm (in ...
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Contrast Media for Plant cells

I currently have a waterweed (elodea) preparation for my optical microscope but i can only see some of the structures (membrane, wall, chloroplasts and the nucleus). Now I want to see more (Like the ...
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Does the Rayleigh formula apply to electron microscopes or only light microscopes?

I am asked to determine the resolving power of both a TEM (transmission electron microscope) and a SEM (scanning electron microscope) and given the Rayleigh formula below. $$Resolution=\frac{0.61\...
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Water sample preservation

I am taking my students to collect water samples from different bodies of water. These bodies of water include the local river, a park pond, and water accumulation from recent rain. Once we gather the ...
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What renders a cytoplasm basophilic?

I know that being basophilic or acidophilic corresponds to affinity to certain dyes used in microscopy. What i want to know is what characteristics of the cytoplasm can we infer from its basophilic ...
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10 votes
1 answer
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Star-like organism seen in microscope

I found this strange star-like thing while observing a slide of onion root somatic cells. I don't know that it is; I even doubt if it is an organism. It has conical arms directed radially outwards ...
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How does Line Integrate work on a laser scanning confocal microscope?

Most microscope software provides options for line average or line integrate as a function that helps the signal to noise ratio when taking an image. The math behind line average seems straight ...
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Can I use a basic compound microscope to analyze the ploidy of plant chromosomes?

So, I'm not really any kind of trained botanist. But I do have two things: some dandelion seeds from France, and, collecting dust in my garage along with related accessories, this thing. Normally, ...
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What does "dissection-based microscopy" mean as described here, and how can it give genetic information?

The Phys.org article 'DNA microscopy' offers entirely new way to image cells references the new Open Access paper in Cell DNA Microscopy: Optics-free Spatio-genetic Imaging by a Stand-Alone Chemical ...
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Quick overview of how to capture microscope images of histology plate or fluorescent plate

I am looking at these two: I would like to DIY take photographs of plant and animal cells using a cheap microscope such as this: I don't know too much about microscopes or the techniques to do the ...
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Could someone identify these microorganisms on microscope?

I'm new in this StackExchange forum, so I don't know if this is an appropriate question. Can someone please help me recognize these micro-organisms on these photo taken with my phone during my ...
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Microscope Recommendations

I am a Engineering University student, in my last year of completion of my degree. I had interests in biochemistry and biology since I was a kid, and been learning about that side by side in my spare ...
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Why are Barr Bodies usually seen along the edge of the nucleus under the light microscope?

We do a typical class exercise of aceto-orsein staining of buccal epithelial cells from female students to visualize Barr bodies under the light microscope. All the illustrations and pictures in the ...
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What's the percentage of zoom needed to see protozoa in an optical microscope?

I was searching for a microscope and I saw some with prices of over 10.000$. However I need to buy one that can actually show me the cells of a culture I am growing at home, and I really need to know ...
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Is it possible to identify this microscopic particle?

So I was playing amateur scientist with my 11 year old. This photograph is from rainwater in a bird-feeding bowl in south England, at 300x. It looks biological, but I have no idea what this is. Is ...
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What do the text annotations in an electron micrograph mean?

I saw a photograph taken from an electron microscope, with some text. I want to know what is the meaning of these numbers and letters? Thank you! Regards.
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Simple and inexpensive geobiochemical studies [closed]

I plan to write a research proposal for conducting a small experiment related to geobiochemistry. I aim to make the experiment: 1) Simple (middle school aged students could comprehend its main ideas),...
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