Questions tagged [microscopy]

The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

Filter by
Sorted by
Tagged with
1
vote
2answers
32 views

Does the Rayleigh formula apply to electron microscopes or only light microscopes?

I am asked to determine the resolving power of both a TEM (transmission electron microscope) and a SEM (scanning electron microscope) and given the Rayleigh formula below. $$Resolution=\frac{0.61\...
1
vote
1answer
34 views

Water sample preservation

I am taking my students to collect water samples from different bodies of water. These bodies of water include the local river, a park pond, and water accumulation from recent rain. Once we gather the ...
1
vote
0answers
5 views

What renders a cytoplasm basophilic?

I know that being basophilic or acidophilic corresponds to affinity to certain dyes used in microscopy. What i want to know is what characteristics of the cytoplasm can we infer from its basophilic ...
10
votes
1answer
91 views

Star-like organism seen in microscope

I found this strange star-like thing while observing a slide of onion root somatic cells. I don't know that it is; I even doubt if it is an organism. It has conical arms directed radially outwards ...
0
votes
1answer
15 views

How does Line Integrate work on a laser scanning confocal microscope?

Most microscope software provides options for line average or line integrate as a function that helps the signal to noise ratio when taking an image. The math behind line average seems straight ...
0
votes
1answer
29 views

Can I use a basic compound microscope to analyze the ploidy of plant chromosomes?

So, I'm not really any kind of trained botanist. But I do have two things: some dandelion seeds from France, and, collecting dust in my garage along with related accessories, this thing. Normally, ...
3
votes
0answers
56 views

What does “dissection-based microscopy” mean as described here, and how can it give genetic information?

The Phys.org article 'DNA microscopy' offers entirely new way to image cells references the new Open Access paper in Cell DNA Microscopy: Optics-free Spatio-genetic Imaging by a Stand-Alone Chemical ...
0
votes
0answers
17 views

Microscope image keeps showing up as background

I’m using Axiovision on a Zeiss Axiocam to look at histology slides under brightfield. There is an unexplained pinkish image that keeps showing even with the light path closed. It’s there in the ...
2
votes
1answer
28 views

Quick overview of how to capture microscope images of histology plate or fluorescent plate

I am looking at these two: I would like to DIY take photographs of plant and animal cells using a cheap microscope such as this: I don't know too much about microscopes or the techniques to do the ...
0
votes
0answers
49 views

Could someone identify these microorganisms on microscope?

I'm new in this StackExchange forum, so I don't know if this is an appropriate question. Can someone please help me recognize these micro-organisms on these photo taken with my phone during my ...
0
votes
0answers
31 views

Microscope Recommendations

I am a Engineering University student, in my last year of completion of my degree. I had interests in biochemistry and biology since I was a kid, and been learning about that side by side in my spare ...
0
votes
0answers
6 views

single microtubule filament tracking

what are the different microscopy techniques used to image single microtubule filaments? How is single filament tracking possible by Total internal reflection fluorescence microscopy (TIRF) and ...
3
votes
0answers
32 views

Why are Barr Bodies usually seen along the edge of the nucleus under the light microscope?

We do a typical class exercise of aceto-orsein staining of buccal epithelial cells from female students to visualize Barr bodies under the light microscope. All the illustrations and pictures in the ...
0
votes
0answers
6 views

Which eukaryotic cellular structures can be resolved by 400x light microscopy

I am wondering which eukaryotic (plant or animal) cellular structures can be resolved by 400x light microscopy (no fluorescence) using standard stains. It appears to me that most smaller compartments ...
-1
votes
1answer
29 views

What's the percentage of zoom needed to see protozoa in an optical microscope?

I was searching for a microscope and I saw some with prices of over 10.000$. However I need to buy one that can actually show me the cells of a culture I am growing at home, and I really need to know ...
0
votes
0answers
6 views

fimbriae under AFM

Can any one,please, recommend a protocol for watching bacteria with fimbriae under atomic force microscope? I have tried a lot but always there were no fimbriae i don't know why.
2
votes
1answer
79 views

Is it possible to identify this microscopic particle?

So I was playing amateur scientist with my 11 year old. This photograph is from rainwater in a bird-feeding bowl in south England, at 300x. It looks biological, but I have no idea what this is. Is ...
2
votes
1answer
50 views

What do the text annotations in an electron micrograph mean?

I saw a photograph taken from an electron microscope, with some text. I want to know what is the meaning of these numbers and letters? Thank you! Regards.
1
vote
0answers
29 views

Simple and inexpensive geobiochemical studies [closed]

I plan to write a research proposal for conducting a small experiment related to geobiochemistry. I aim to make the experiment: 1) Simple (middle school aged students could comprehend its main ideas),...
0
votes
0answers
32 views

Is it normal to see yeast cells stacked in the Z axis when counting yeast?

I just got started with yeast cell counting and was surprised to observe yeast cells that are at different z axis depths at 400x. That is, by adjusting the focus (not the x/y stage) I can make some ...
0
votes
1answer
43 views

How are light sheet microscopy 3D images and their computed sections built up from stacks of 2D images?

The iBiology Techniques video Microscopy: Dual-View Inverted Selective Plane Illumination (diSPIM) (Hari Shroff) describes light sheet microscopy and the improvement in resolution by introducing dual-...
1
vote
1answer
150 views

Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
6
votes
0answers
48 views

Does cheese contain cells of animal stomachs?

When cheese is made with calf rennet, is it possible that the cheese contains cells of this calf (stomach)? Dried and cleaned stomachs of young calves are sliced into small pieces and then put into ...
-1
votes
1answer
211 views

What does “dissipated” mean here?

This is the context: You know cardiac muscle cells are small cells relative to skeletal muscle cells, but unlike skeletal muscle cells that are arranged in parallel and their contraction is ...
2
votes
1answer
26 views

Do different microscopes and attached cameras differ in crop factor between each other?

I analyzed a bunch of microphotographs recently and used "% of image area" to describe the area of structures (chorionic villi, had to show their density in placenta). However my professor suggested I ...
3
votes
1answer
76 views

Sample suggestions for phase contrast microscopy

I am a non-bio student working on an academic paper recently and would like to have some sample preparation for phase contrast microscope. For it to work, I imagine the sample to be: Transparent; ...
1
vote
1answer
64 views

Microscope parameters for whole blood alalysis

I wonder, what are the minimum requirements for a microscope for blood cell analysis. I'm interested in: -Counting erythrocytes -Counting and differenciating all typed of leucocytes -Counting of ...
2
votes
3answers
745 views

What can I see with a microscope at what magnifications?

I'm 26 and where I grew up the schools were complete crap so I've never really used a microscope much or learned much about them. And I want to learn some science and look at some microscopic things, ...
3
votes
0answers
48 views

Antibody staining

I am staining tissue sections and I did a mistake, I was supposed to have mixed 3 primary antibodies but I stained only with one of them. After the 1h incubation I washed 10 min with PBS and then I ...
0
votes
1answer
299 views

Comparison between Transmission Electron Microscope (TEM) to Scanning Electron Microscope (SEM)

Can someone state the advantages of Transmission Electron Microscope (TEM) over Scanning Electron Microscope (SEM) and vice versa?
2
votes
1answer
793 views

Why shouldn't I clean microscope slides with a paper towel?

I've recently bought a microscope and been looking at a lot of bacteria and such, and have quickly run out of clean slides. I've seen videos and articles saying how you shouldn't clean microscope ...
2
votes
1answer
486 views

Identification of protozoa under microscope

I observed maybe Protozoa from standing FRESH water and from slowly flowing FRESH water. I am complete dilettante. Can you tell what these creatures are? https://www.youtube.com/watch?v=6D5ck3zNJzA&...
-1
votes
1answer
59 views

Minimum hardware and knowledge to study effects on live cells [closed]

I'm very interested to study the effects (or reactions) of different forces (magnetic fields, electric fields, etc) on living tissues/cells. I know I have to have a living cell, the force applied and ...
0
votes
2answers
453 views

Can you use a microscope without an eyepiece? [closed]

Can you use a microscope without an eyepiece?
1
vote
0answers
61 views

Scanning Electron Microscope images of wheat grains

I took SEM images of wheat grains but I see some granular particles on its surface and I don't understand what they are? Is it some kind of deformation of the bran layer due to drying? Or is it the ...
1
vote
0answers
134 views

Why there is no fluorescent light from my DAPI-stained Hibiscus Rosa Sinensis Pollen?

I currently work with fluorescence microscope to observe generative and vegetative cells within hibiscus rosa sinensis pollen. I used DAPI to stain pollens but there is no light that indicate the ...
0
votes
1answer
63 views

Where do i find microbes like tardigrades in winter

Just bought a microscope and i want to see microbes like tardigrades. Where should i look? I have found many bacteria and what seems to be motionless single celled organism. I want to see movement ...
1
vote
2answers
216 views

How do I look for pathogen in water with a microscope? Is it possible?

I am a 9th grade student from Manila and we're currently working on an investigatory project. We want to know how effective SODIS or solar water disinfection is. The purification method claims to be ...
2
votes
0answers
80 views

What is this thing found in my saliva?

Found in my saliva (Optical microscope 40x)
1
vote
0answers
71 views

Dark and bright sides in fluorescence microscopy images?

I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that one part of image ...
2
votes
0answers
265 views

Common stains for fungal microscopy

I recently got a light microscope to start exploring microscopic features of fungi (and to explore microscopy in general). What are some of the most common and easily available stains used when ...
-1
votes
1answer
573 views

Onion Cells or Pear Cells?

I am researching different cells under a brightfield microscope for homework and am having trouble determining whether or not these cells are Onion cells (Allium cepa) or pear cells (Pyrus) under a ...
1
vote
1answer
91 views

Opening coverslip package

I feel pretty silly for asking this but I don't want to damage these coverslips. I just ordered a 100ct box of coverslips and see no way to open them without destroying the container they are in. How ...
1
vote
0answers
47 views

How can I retain cells in suspension for comparative microscopy?

I am working on a digital holographic microscope. I would like to compare its imaging properties with an actual phase contrast light microscope. My aim is to do this cell by cell, creating a z-stack ...
3
votes
0answers
54 views

Why do I see so many kinetochores?

I am analysing RPE-1 cells from humans and I do not understand why I see so many kinetochores by immunofluorescence (more than 100 in many cells). They are in prometaphase.
2
votes
1answer
225 views

Is there any way to differentiate between a contractile and a non contractile vacuole by observation alone in any given organism

I have read about distinguishing them using osmotic experiments in books and here as well. But is there any thing common (on a biochemistry level) from which I can tell if a vacuole is contractile or ...
1
vote
0answers
3k views

I can't see anything at all in my microscope

I just got the Amscope M102C microscope, and it is the first microscope I've operated. I decided to try to look at a fly leg at 40x magnification, but all I could see were the blobs in your eye you ...
3
votes
0answers
53 views

Please help Identify these algae [closed]

Found this algae in a small stony stream that feeds a medium sized river. I can not identify either of the filamentous algae in this photo
2
votes
1answer
93 views

What kind of microorganism is this?

It moves like a worm pretty much. I found it in a pretty big pond with brackish water. The water temperature was around 10 celsius. Here's a video of it moving
4
votes
0answers
42 views

Can I just simply exchange the eyepiece in a microscope?

I hope it's the right place to ask about microscopy. So my question is what parameters do I have to take into account when buying a new eyepiece for my microscope? Currently I own an Aomekie XSP-42 ...