Questions tagged [microscopy]

The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

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41 views

How to take professional SEM images of bacteria?

I am new to using SEM microscope I would like to take clear pictures of Bacillus subtilis. My sample is dropped on a piece of paper and carbon-coated. The picture is showing crumbs of something (maybe ...
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Cannot seem to merge the two circles of a microscope (biological, binocular)

I have recently purchased a binocular microscope (Saxon Researcher Compact Biological Microscope 40x-1600x), and have had difficulty getting the two circles of light that I'm seeing through the ...
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What are the small white dots in an onion cell seen under an electron microscope?

We had to use an electron microscope in class for an assignment and used onion cells. On the 40x and 100x magnification there are tiny white dots and I was wondering if someone could tell me what they ...
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Why do bacteriophages look like tiny little robots? [closed]

I get it, evolution is funny and works in mysterious ways. But come on. You can't tell me this thing doesn't look like a nanobot. Why do bacteriophages look this way? With their cylindrical heads and ...
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Microorganism ID?

I found this microorganism in a wet moss sample. It was very active and moved around quite quickly, and it looked to be using cilia to move. It had no coloration. I got some pictures at 400x: Any ...
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ID microbe found in moss sample (USA)

I found this microbe after putting a wet moss sample under my microscope. It latched on to a piece of moss and scanned the area around it for prey, and when it didn't find any, it slowly swam away. It'...
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Reference for standardized histology rating criteria of microscope images

I am looking for a reference to a standard protocol for assessing the visual quality and diagnostic potential of a tissue sample that has been processed and stained for viewing under light microscope. ...
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Is this a real "double microscope" with two stages and two sets of objectives? Is it used in biology?

In this US late night TV broadcast1 a set has been created with some "science objects" for visual impact, and right in the center is an unusual looking optical microscope. The bottom looks ...
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colocalization by IFA?

I have done IFA (immunofluorescence assay) previously but i targeted only one protein or i tagged my endogenous protein with GFP/RFP then i performed IFA. I want to do the IFA in parental cell line (...
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How to select a microscope for deep microscopy? Specifically for Deep Learning/ML model development

I am new to biology and microscopy. I am a data scientist. I am faced with a question on selecting a good microscope that enables automation of scanning the slides. And cellular object detection using ...
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Can GFP reporting be used to track localization of peptides in the ER, Golgi, and plasma membrane?

Suppose I want to study the trafficking of a peptide throughout the ER, Golgi, and plasma membrane. An idea I had was labeling a secreted or plasma membrane integral protein with GFP and using time-...
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Recommendations for a haemocytometer (phase-contrast)

I'm looking for recommendations for a haemocytometer that can be used with a phase-contrast microscope. I want to use it to quantify the vegetative and spore fraction in my samples. Can I use any ...
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139 views

What does it mean to "cure" microscope slides?

I am reading a journal paper and I have come across the following statement in the materials and methods section regarding the preparation of microscope slides: Coverslips were washed once more with ...
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50 views

Why are cells in blood smears undetectable after absorbing moisture?

I'm a student learning how to prepare blood films and today I was curious about something, so I performed an experiment. After preparing a thin blood smear and drying it (no staining), I took a look ...
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Do electron microscopy change the structure of the specimen due to the vacuum step?

Since specimens have to be prepared using the vacuum, would the pressure change affect the structures of the specimen?
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What are these structures in blood from a nosebleed?

I decided to look at some blood from a nosebleed (not mine) under the microscope and took some pictures of the results. There many large orange structures interspersed within the blood cells and I'm ...
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Is there any reason to use an upright microscope over an inverted microscope?

I've never actually owned an inverted microscope, but it seems it has only advantages compared to an upright microscope: taller, heavier samples; no crashing the objective into the glass slide; easier ...
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How long do Ziehl-Neelsen stained samples keep?

I have acquired TB slides from my city's health center that are supposedly acid-fast bacteria (AFB) positive. However, when I took a look at it, I see basically no carbol fuchsin stains. They were ...
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Is the dehydration process used twice in the preparation of permanent slides?

I am taught "preparation of permanent slides" by one of my teachers in the course of Biological Techniques. He gave me the following steps to prepare a permanent slide: Totally, there are ...
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Can anyone ID this microorganism?

I found this microorganism in a wet moss sample taken from my backyard in Richmond, Virginia (USA). I took some pictures of it at 40x, 100x, and 250x. I'm not entirely sure what it is, but I think it'...
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53 views

Ferns: How big can a circular sorus be?

I looked at some small ferns under the microscope. The circular sori of the ferns I looked at were less than 2 mm in diameter. The microscope I used had rather weak magnification, so I didn't get to ...
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Is a FoldScope microscope suitable for plant cytogenetics?

I have recently been getting more interested in amateur botany, and have some background in genetics and genomics (PhD+). I have used standard light and confocal microscopes but don't have much ...
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umbrella term for microtiter plate, petri dish, cell culture flask etc

I'm looking for a term I can use in a software user interface that includes anything that may carry cell cultures or similar biological imaging samples (ie that may conceivably go into an automated ...
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31 views

Image Dataset for Cardiac cells for Mus musculus?

I'm working on developing a transfer learning model to detect cells in a culture using microscopic images. for that i require data set of images having microscopic images of various cell types. Is ...
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59 views

Transmitted electrons in TEM

When some electrons of the electron beam pass through the specimen, what exactly happens after that in order to produce the black white image? What I understand is that electrons transmitted collide ...
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61 views

How to obtain raphides from plants?

I recently found out that certain plants produce needle-shaped crystals called raphides. I am a physicist and I have an experiment in mind that could use these needles, but I would need to extract ...
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229 views

Is a wet-mount needed to use Immersion Oil?

When I prepare my slide to use immersion oil, do I really need to wet-mount the specimen? Can I do it with just the coverslip? I am preparing fungus spores. Using water for the wet-mount gives you ...
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Nuclear vs Cytoplasmic Fluorescence [closed]

When staining transciption factor proteins with fluorescent antibodies (Alexa, etc.), why are the fluorescence signals stronger in the nucleus as compared to fluorescence signals in the cytoplasm (in ...
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Contrast Media for Plant cells

I currently have a waterweed (elodea) preparation for my optical microscope but i can only see some of the structures (membrane, wall, chloroplasts and the nucleus). Now I want to see more (Like the ...
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161 views

Does the Rayleigh formula apply to electron microscopes or only light microscopes?

I am asked to determine the resolving power of both a TEM (transmission electron microscope) and a SEM (scanning electron microscope) and given the Rayleigh formula below. $$Resolution=\frac{0.61\...
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53 views

Water sample preservation

I am taking my students to collect water samples from different bodies of water. These bodies of water include the local river, a park pond, and water accumulation from recent rain. Once we gather the ...
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What renders a cytoplasm basophilic?

I know that being basophilic or acidophilic corresponds to affinity to certain dyes used in microscopy. What i want to know is what characteristics of the cytoplasm can we infer from its basophilic ...
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271 views

Star-like organism seen in microscope

I found this strange star-like thing while observing a slide of onion root somatic cells. I don't know that it is; I even doubt if it is an organism. It has conical arms directed radially outwards ...
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93 views

How does Line Integrate work on a laser scanning confocal microscope?

Most microscope software provides options for line average or line integrate as a function that helps the signal to noise ratio when taking an image. The math behind line average seems straight ...
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Can I use a basic compound microscope to analyze the ploidy of plant chromosomes?

So, I'm not really any kind of trained botanist. But I do have two things: some dandelion seeds from France, and, collecting dust in my garage along with related accessories, this thing. Normally, ...
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What does "dissection-based microscopy" mean as described here, and how can it give genetic information?

The Phys.org article 'DNA microscopy' offers entirely new way to image cells references the new Open Access paper in Cell DNA Microscopy: Optics-free Spatio-genetic Imaging by a Stand-Alone Chemical ...
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Quick overview of how to capture microscope images of histology plate or fluorescent plate

I am looking at these two: I would like to DIY take photographs of plant and animal cells using a cheap microscope such as this: I don't know too much about microscopes or the techniques to do the ...
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Could someone identify these microorganisms on microscope?

I'm new in this StackExchange forum, so I don't know if this is an appropriate question. Can someone please help me recognize these micro-organisms on these photo taken with my phone during my ...
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Microscope Recommendations

I am a Engineering University student, in my last year of completion of my degree. I had interests in biochemistry and biology since I was a kid, and been learning about that side by side in my spare ...
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Why are Barr Bodies usually seen along the edge of the nucleus under the light microscope?

We do a typical class exercise of aceto-orsein staining of buccal epithelial cells from female students to visualize Barr bodies under the light microscope. All the illustrations and pictures in the ...
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What's the percentage of zoom needed to see protozoa in an optical microscope?

I was searching for a microscope and I saw some with prices of over 10.000$. However I need to buy one that can actually show me the cells of a culture I am growing at home, and I really need to know ...
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Is it possible to identify this microscopic particle?

So I was playing amateur scientist with my 11 year old. This photograph is from rainwater in a bird-feeding bowl in south England, at 300x. It looks biological, but I have no idea what this is. Is ...
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What do the text annotations in an electron micrograph mean?

I saw a photograph taken from an electron microscope, with some text. I want to know what is the meaning of these numbers and letters? Thank you! Regards.
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Simple and inexpensive geobiochemical studies [closed]

I plan to write a research proposal for conducting a small experiment related to geobiochemistry. I aim to make the experiment: 1) Simple (middle school aged students could comprehend its main ideas),...
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Is it normal to see yeast cells stacked in the Z axis when counting yeast?

I just got started with yeast cell counting and was surprised to observe yeast cells that are at different z axis depths at 400x. That is, by adjusting the focus (not the x/y stage) I can make some ...
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How are light sheet microscopy 3D images and their computed sections built up from stacks of 2D images?

The iBiology Techniques video Microscopy: Dual-View Inverted Selective Plane Illumination (diSPIM) (Hari Shroff) describes light sheet microscopy and the improvement in resolution by introducing dual-...
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Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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Does cheese contain cells of animal stomachs?

When cheese is made with calf rennet, is it possible that the cheese contains cells of this calf (stomach)? Dried and cleaned stomachs of young calves are sliced into small pieces and then put into ...
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What does "dissipated" mean here?

This is the context: You know cardiac muscle cells are small cells relative to skeletal muscle cells, but unlike skeletal muscle cells that are arranged in parallel and their contraction is ...
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Do different microscopes and attached cameras differ in crop factor between each other?

I analyzed a bunch of microphotographs recently and used "% of image area" to describe the area of structures (chorionic villi, had to show their density in placenta). However my professor suggested I ...