Questions tagged [microscopy]

The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

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Microscope image keeps showing up as background

I’m using Axiovision on a Zeiss Axiocam to look at histology slides under brightfield. There is an unexplained pinkish image that keeps showing even with the light path closed. It’s there in the ...
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1answer
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Quick overview of how to capture microscope images of histology plate or fluorescent plate

I am looking at these two: I would like to DIY take photographs of plant and animal cells using a cheap microscope such as this: I don't know too much about microscopes or the techniques to do the ...
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1answer
127 views

Killing microorganisms on agar plates?

If I grow microorganisms on agar plates and I expose them to UV light. How exactly will I know that the microorganisms have been killed? I was watching a video that showed spots in the petri dish ...
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Could someone identify these microorganisms on microscope?

I'm new in this StackExchange forum, so I don't know if this is an appropriate question. Can someone please help me recognize these micro-organisms on these photo taken with my phone during my ...
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Microscope Recommendations

I am a Engineering University student, in my last year of completion of my degree. I had interests in biochemistry and biology since I was a kid, and been learning about that side by side in my spare ...
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single microtubule filament tracking

what are the different microscopy techniques used to image single microtubule filaments? How is single filament tracking possible by Total internal reflection fluorescence microscopy (TIRF) and ...
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1answer
3k views

What is the magnification necessary to see pond life

My daughter is 5 and wants to learn about Biology. I thought a great start is to see life from pond water through a USB microscope. It would be great if she could see organisms like amoebas, ...
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Why are Barr Bodies usually seen along the edge of the nucleus under the light microscope?

We do a typical class exercise of aceto-orsein staining of buccal epithelial cells from female students to visualize Barr bodies under the light microscope. All the illustrations and pictures in the ...
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1answer
154 views

Is there any way to differentiate between a contractile and a non contractile vacuole by observation alone in any given organism

I have read about distinguishing them using osmotic experiments in books and here as well. But is there any thing common (on a biochemistry level) from which I can tell if a vacuole is contractile or ...
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Which eukaryotic cellular structures can be resolved by 400x light microscopy

I am wondering which eukaryotic (plant or animal) cellular structures can be resolved by 400x light microscopy (no fluorescence) using standard stains. It appears to me that most smaller compartments ...
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1answer
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What's the percentage of zoom needed to see protozoa in an optical microscope?

I was searching for a microscope and I saw some with prices of over 10.000$. However I need to buy one that can actually show me the cells of a culture I am growing at home, and I really need to know ...
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6 views

fimbriae under AFM

Can any one,please, recommend a protocol for watching bacteria with fimbriae under atomic force microscope? I have tried a lot but always there were no fimbriae i don't know why.
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Simple and inexpensive geobiochemical studies [closed]

I plan to write a research proposal for conducting a small experiment related to geobiochemistry. I aim to make the experiment: 1) Simple (middle school aged students could comprehend its main ideas),...
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1answer
275 views

Comparison between Transmission Electron Microscope (TEM) to Scanning Electron Microscope (SEM)

Can someone state the advantages of Transmission Electron Microscope (TEM) over Scanning Electron Microscope (SEM) and vice versa?
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1answer
76 views

Is it possible to identify this microscopic particle?

So I was playing amateur scientist with my 11 year old. This photograph is from rainwater in a bird-feeding bowl in south England, at 300x. It looks biological, but I have no idea what this is. Is ...
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1answer
48 views

What do the text annotations in an electron micrograph mean?

I saw a photograph taken from an electron microscope, with some text. I want to know what is the meaning of these numbers and letters? Thank you! Regards.
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Is it normal to see yeast cells stacked in the Z axis when counting yeast?

I just got started with yeast cell counting and was surprised to observe yeast cells that are at different z axis depths at 400x. That is, by adjusting the focus (not the x/y stage) I can make some ...
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1answer
40 views

How are light sheet microscopy 3D images and their computed sections built up from stacks of 2D images?

The iBiology Techniques video Microscopy: Dual-View Inverted Selective Plane Illumination (diSPIM) (Hari Shroff) describes light sheet microscopy and the improvement in resolution by introducing dual-...
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Does cheese contain cells of animal stomachs?

When cheese is made with calf rennet, is it possible that the cheese contains cells of this calf (stomach)? Dried and cleaned stomachs of young calves are sliced into small pieces and then put into ...
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1answer
365 views

Mites on ginger root

I was just looking at some mouldy ginger found in the fridge under the microscope and was horrified/delighted to find (what looked to be) mites growing in it. I have searched the web and can only find ...
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2answers
277 views

Atomic force microscopy: depth penetration?

What depth penetration can one typically get with atomic force microscopes? (i.e. how far into the tissue can one image?). I have found numbers on acquisition times (minutes) and spatial resolutions (...
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1answer
25 views

Do different microscopes and attached cameras differ in crop factor between each other?

I analyzed a bunch of microphotographs recently and used "% of image area" to describe the area of structures (chorionic villi, had to show their density in placenta). However my professor suggested I ...
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3answers
3k views

What is the best way to clean immersion oil from the microscope lens?

My job is to photograph mushroom spores at x100 using immersion oil. What is the best practice for cleaning the oil from the lens? Right now I am just wiping it with a cotton swab.
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179 views

What does “dissipated” mean here?

This is the context: You know cardiac muscle cells are small cells relative to skeletal muscle cells, but unlike skeletal muscle cells that are arranged in parallel and their contraction is ...
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1answer
55 views

Sample suggestions for phase contrast microscopy

I am a non-bio student working on an academic paper recently and would like to have some sample preparation for phase contrast microscope. For it to work, I imagine the sample to be: Transparent; ...
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1answer
63 views

Microscope parameters for whole blood alalysis

I wonder, what are the minimum requirements for a microscope for blood cell analysis. I'm interested in: -Counting erythrocytes -Counting and differenciating all typed of leucocytes -Counting of ...
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2answers
4k views

Microscope recommendation for an amateur?

I am a mathematics student looking for a hobby and this has come up as a potential hobby. Thus I am interested in buying a high-magnification microscope that is within the limits of my budget. My ...
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3answers
458 views

What can I see with a microscope at what magnifications?

I'm 26 and where I grew up the schools were complete crap so I've never really used a microscope much or learned much about them. And I want to learn some science and look at some microscopic things, ...
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48 views

Antibody staining

I am staining tissue sections and I did a mistake, I was supposed to have mixed 3 primary antibodies but I stained only with one of them. After the 1h incubation I washed 10 min with PBS and then I ...
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1answer
658 views

Why shouldn't I clean microscope slides with a paper towel?

I've recently bought a microscope and been looking at a lot of bacteria and such, and have quickly run out of clean slides. I've seen videos and articles saying how you shouldn't clean microscope ...
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2answers
177 views

How do I look for pathogen in water with a microscope? Is it possible?

I am a 9th grade student from Manila and we're currently working on an investigatory project. We want to know how effective SODIS or solar water disinfection is. The purification method claims to be ...
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1answer
435 views

Identification of protozoa under microscope

I observed maybe Protozoa from standing FRESH water and from slowly flowing FRESH water. I am complete dilettante. Can you tell what these creatures are? https://www.youtube.com/watch?v=6D5ck3zNJzA&...
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1answer
59 views

Minimum hardware and knowledge to study effects on live cells [closed]

I'm very interested to study the effects (or reactions) of different forces (magnetic fields, electric fields, etc) on living tissues/cells. I know I have to have a living cell, the force applied and ...
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2answers
408 views

Can you use a microscope without an eyepiece? [closed]

Can you use a microscope without an eyepiece?
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123 views

Why there is no fluorescent light from my DAPI-stained Hibiscus Rosa Sinensis Pollen?

I currently work with fluorescence microscope to observe generative and vegetative cells within hibiscus rosa sinensis pollen. I used DAPI to stain pollens but there is no light that indicate the ...
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Scanning Electron Microscope images of wheat grains

I took SEM images of wheat grains but I see some granular particles on its surface and I don't understand what they are? Is it some kind of deformation of the bran layer due to drying? Or is it the ...
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1answer
62 views

Where do i find microbes like tardigrades in winter

Just bought a microscope and i want to see microbes like tardigrades. Where should i look? I have found many bacteria and what seems to be motionless single celled organism. I want to see movement ...
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4answers
417 views

Super-resolution imaging in vivo

Are there any microscopy modalities or techniques for in vivo imaging at higher resolutions than the diffraction limit? I was looking at this list: Super resolution microscopy, but they don't talk ...
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2answers
495 views

Can living cells be imaged with electron microscopy?

According to this article from October 2009, http://news.mit.edu/2009/electron-microscope "Electron microscopes are the most powerful type of microscope, capable of distinguishing even individual ...
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79 views

What is this thing found in my saliva?

Found in my saliva (Optical microscope 40x)
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63 views

Dark and bright sides in fluorescence microscopy images?

I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that one part of image ...
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1answer
476 views

Onion Cells or Pear Cells?

I am researching different cells under a brightfield microscope for homework and am having trouble determining whether or not these cells are Onion cells (Allium cepa) or pear cells (Pyrus) under a ...
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243 views

Common stains for fungal microscopy

I recently got a light microscope to start exploring microscopic features of fungi (and to explore microscopy in general). What are some of the most common and easily available stains used when ...
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1answer
63 views

Opening coverslip package

I feel pretty silly for asking this but I don't want to damage these coverslips. I just ordered a 100ct box of coverslips and see no way to open them without destroying the container they are in. How ...
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0answers
47 views

How can I retain cells in suspension for comparative microscopy?

I am working on a digital holographic microscope. I would like to compare its imaging properties with an actual phase contrast light microscope. My aim is to do this cell by cell, creating a z-stack ...
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1answer
601 views

What is going on in these cells?

Here are some pictures of human iPS cells in culture. After several passages, some cells look really weird, can you help me understand what is going on? What am I looking at? What are those blastocyst-...
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0answers
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Why do I see so many kinetochores?

I am analysing RPE-1 cells from humans and I do not understand why I see so many kinetochores by immunofluorescence (more than 100 in many cells). They are in prometaphase.
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3k views

I can't see anything at all in my microscope

I just got the Amscope M102C microscope, and it is the first microscope I've operated. I decided to try to look at a fly leg at 40x magnification, but all I could see were the blobs in your eye you ...
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0answers
53 views

Please help Identify these algae [closed]

Found this algae in a small stony stream that feeds a medium sized river. I can not identify either of the filamentous algae in this photo
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1answer
509 views

Is Zeiss Primo Start microscope worth the price for student use? [closed]

I study physics but I have got interested about biology too. Surfing around the web, I have found out that in buying microscopes one needs to decide between the cheaper no-name brands vs. the four big ...