Questions tagged [microscopy]

The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

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Does cheese contain cells of animal stomachs?

When cheese is made with calf rennet, is it possible that the cheese contains cells of this calf (stomach)? Dried and cleaned stomachs of young calves are sliced into small pieces and then put into ...
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Can I just simply exchange the eyepiece in a microscope?

I hope it's the right place to ask about microscopy. So my question is what parameters do I have to take into account when buying a new eyepiece for my microscope? Currently I own an Aomekie XSP-42 ...
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How can I keep pond water “alive”?

I've been preparing wet slides for my kids to look at from pond water. I have a mason jar of pond water. What can I do to keep the microbes in the jar alive going forward so that we can look at it any ...
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What does “dissection-based microscopy” mean as described here, and how can it give genetic information?

The Phys.org article 'DNA microscopy' offers entirely new way to image cells references the new Open Access paper in Cell DNA Microscopy: Optics-free Spatio-genetic Imaging by a Stand-Alone Chemical ...
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Why are Barr Bodies usually seen along the edge of the nucleus under the light microscope?

We do a typical class exercise of aceto-orsein staining of buccal epithelial cells from female students to visualize Barr bodies under the light microscope. All the illustrations and pictures in the ...
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Antibody staining

I am staining tissue sections and I did a mistake, I was supposed to have mixed 3 primary antibodies but I stained only with one of them. After the 1h incubation I washed 10 min with PBS and then I ...
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Why do I see so many kinetochores?

I am analysing RPE-1 cells from humans and I do not understand why I see so many kinetochores by immunofluorescence (more than 100 in many cells). They are in prometaphase.
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Microscopy Book Suggestions

I've learned programming through great book recommendations; many from the Stack Exchange series of sites. I'm hoping to take this approach in order to gain a fundamental understanding of how ...
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Can anyone identify this microorganism(s)?

I took a sample of water squeezed out of pincushion moss and put it under my microscope at a range of 100-250x and found this strange little organism. At first, I thought it was some kind of algae due ...
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Contrast Media for Plant cells

I currently have a waterweed (elodea) preparation for my optical microscope but i can only see some of the structures (membrane, wall, chloroplasts and the nucleus). Now I want to see more (Like the ...
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What is this thing found in my saliva?

Found in my saliva (Optical microscope 40x)
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Common stains for fungal microscopy

I recently got a light microscope to start exploring microscopic features of fungi (and to explore microscopy in general). What are some of the most common and easily available stains used when ...
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279 views

Light microscope specimen size

I was going through sample questions for Science Olympiad, and one of the questions went like this: A cell is observed through a light microscope at 4x magnification. The cell takes up about half ...
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background extraction in microscope analysis

I have taken microscope pictures for proteins in HeLa cells, in order to quantify the intensity (immunofluorescence). The proteins are both at the nuclear envelope, and in order to quantify the ...
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Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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643 views

Are there any drawbacks to having a fixed-Köhler microscope?

I am considering to buy the Zeiss Primo star microscope. There are two variants: one with so called "Full Köhler" illumination and one with "Fixed-Köhler" illumination. Are there any drawbacks to ...
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umbrella term for microtiter plate, petri dish, cell culture flask etc

I'm looking for a term I can use in a software user interface that includes anything that may carry cell cultures or similar biological imaging samples (ie that may conceivably go into an automated ...
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What renders a cytoplasm basophilic?

I know that being basophilic or acidophilic corresponds to affinity to certain dyes used in microscopy. What i want to know is what characteristics of the cytoplasm can we infer from its basophilic ...
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Scanning Electron Microscope images of wheat grains

I took SEM images of wheat grains but I see some granular particles on its surface and I don't understand what they are? Is it some kind of deformation of the bran layer due to drying? Or is it the ...
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Why there is no fluorescent light from my DAPI-stained Hibiscus Rosa Sinensis Pollen?

I currently work with fluorescence microscope to observe generative and vegetative cells within hibiscus rosa sinensis pollen. I used DAPI to stain pollens but there is no light that indicate the ...
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Dark and bright sides in fluorescence microscopy images?

I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that one part of image ...
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How can I retain cells in suspension for comparative microscopy?

I am working on a digital holographic microscope. I would like to compare its imaging properties with an actual phase contrast light microscope. My aim is to do this cell by cell, creating a z-stack ...
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I can't see anything at all in my microscope

I just got the Amscope M102C microscope, and it is the first microscope I've operated. I decided to try to look at a fly leg at 40x magnification, but all I could see were the blobs in your eye you ...
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Slicing cell cultures frozen in vitrified ice with a laser for CryoEM

Could a cell culture frozen in vitrified ice for CryoEM be effectively sliced into thin planes and resolved images produced? I was thinking if this could be done to get images from inside a cell.
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Can anyone explain in a simple manner what is Fourier shell correlation (FSC)? And how is the conversion from 1D to 3D FSC?

3D FSC curves are used to show directional isotropy and global resolutions in cryo-EM maps. I am having a difficulty to understand the graphs since I do not have absolute clear idea about 3D FSC and ...
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Would it be possible to determine the strength of each neural connection in a connectome data set?

I understand that a connectome is a map of connections between neurons. Would it be possible with current day technology to create a more detailed map that gives not only the connections themselves ...
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Image Dataset for Cardiac cells for Mus musculus?

I'm working on developing a transfer learning model to detect cells in a culture using microscopic images. for that i require data set of images having microscopic images of various cell types. Is ...
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Eyepiece graticule question

In my textbook for Biology A level, it says that the "relative size of the divisions in the eyepiece graticule increases with magnification". What does this mean? I thought that the value which each ...
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Could someone identify these microorganisms on microscope?

I'm new in this StackExchange forum, so I don't know if this is an appropriate question. Can someone please help me recognize these micro-organisms on these photo taken with my phone during my ...
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Microscope Recommendations

I am a Engineering University student, in my last year of completion of my degree. I had interests in biochemistry and biology since I was a kid, and been learning about that side by side in my spare ...
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Is it normal to see yeast cells stacked in the Z axis when counting yeast?

I just got started with yeast cell counting and was surprised to observe yeast cells that are at different z axis depths at 400x. That is, by adjusting the focus (not the x/y stage) I can make some ...
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455 views

Effects of Fixation on Cell Membrane

Why does fixing of cells with something like formaldehyde disrupt the membrane enough for probes, antibodies, and dyes to get into the cell?
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51 views

How to increase contrast with only a top entry specimen holder

My question is: If my microscope had a top entry specimen holder, instead of a side entry holder, what additional option(s) should I have to increase the contrast with the same constraint that I only ...
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What's the percentage of zoom needed to see protozoa in an optical microscope?

I was searching for a microscope and I saw some with prices of over 10.000$. However I need to buy one that can actually show me the cells of a culture I am growing at home, and I really need to know ...