Questions tagged [microscopy]

The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

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Machine learning for light microscopy -- problems to solve?

I would like solve some biological problems that would improve the state-of-the-art of biology or bioinformatics. In particular, I want to apply machine learning on light microscopic images. The ...
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20 votes
1 answer
630 views

What is going on in these cells?

Here are some pictures of human iPS cells in culture. After several passages, some cells look really weird, can you help me understand what is going on? What am I looking at? What are those blastocyst-...
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19 votes
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What magnification do I need to see blood cells?

If I want to buy a microscope for my kids to be able to view single celled creatures and blood cells, about what magnification is required? A Celestron Pentaview digital scope claims up to 600×. Is ...
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18 votes
2 answers
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What is the magnification necessary to see pond life

My daughter is 5 and wants to learn about Biology. I thought a great start is to see life from pond water through a USB microscope. It would be great if she could see organisms like amoebas, ...
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15 votes
4 answers
891 views

Online repositories of scanning electron microscope photographs?

I have looked at every single Wikimedia commons photo in the category 'scanning electron microscope'. Unfortunately, I could not find any other repositories of such photos, but I would readily look at ...
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1 answer
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Can anyone ID this microorganism?

I found this microorganism in a wet moss sample taken from my backyard in Richmond, Virginia (USA). I took some pictures of it at 40x, 100x, and 250x. I'm not entirely sure what it is, but I think it'...
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11 votes
3 answers
1k views

What to look for when buying a light microscope?

I am a science lover and want to buy a microscope to explore things around me. Like studying cell structures, microorganisms, blood and plants. It would be great if there is way to photograph slides. ...
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10 votes
1 answer
351 views

Star-like organism seen in microscope

I found this strange star-like thing while observing a slide of onion root somatic cells. I don't know that it is; I even doubt if it is an organism. It has conical arms directed radially outwards ...
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Microscope recommendation for an amateur?

I am a mathematics student looking for a hobby and this has come up as a potential hobby. Thus I am interested in buying a high-magnification microscope that is within the limits of my budget. My ...
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8 votes
1 answer
82 views

To which thing does "two-thirds" refer here?

Life and Letters of Charles Darwin, volume 2 includes the following letter from Darwin to Hooker, about a examining a plant that absorbs ammonia:- Reflect how little ammonia a plant can get growing ...
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7 votes
1 answer
36k views

How do I identify the different stages of meiosis under microscope?

This is a question I'm troubled with as my exams are knocking at the door. I need help so I decided to post this here. Here's what I know and understand about meiotic stages: Specimen: Grasshopper (...
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6 votes
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What kind of microscope for ML/biological research?

I am a computer science student, focusing on machine learning applications. I have been always interested in biology but I lack any training in it. Now, I had an idea that I could introduce myself to ...
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4 answers
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Super-resolution imaging in vivo

Are there any microscopy modalities or techniques for in vivo imaging at higher resolutions than the diffraction limit? I was looking at this list: Super resolution microscopy, but they don't talk ...
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6 votes
3 answers
13k views

Is it true that viruses cannot be seen with phase-contrast microscopy?

There appears to be a lot of material on the internet claiming that viruses can be only seen with electron microscopes, and not with light microscopes. To the contrary, for example this old paper ...
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2 answers
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What is the state of automated quantitative analysis?

Quantitative analysis of microscope images is essential for many types of studies, but the techniques used most often are very labor-intensive. I have been able to find some literature about using ...
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6 votes
1 answer
180 views

What Websites Have Image Libraries for Bacteria and other Microorganisms

What Websites Have Image Libraries for Bacteria and other Microorganisms? With age of cell phone microscopes and hand held spectrometers it would be interesting and valuable to be able to compare in-...
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What is a good source that will help me classify protists?

I am a 15 year old that enjoys looking at the micro world. The only issue that I have is that I can't find a good online source that will help me classify the name of the micro organism that I'm ...
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5 votes
2 answers
450 views

Dyes to bind to double stranded DNA?

Are there any commercially available fluorescent dyes that will bind only to double stranded DNA (not RNA, single stranded DNA etc.) for studying in vitro using confocal microscopy?
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Can living cells be imaged with electron microscopy?

According to this article from October 2009, http://news.mit.edu/2009/electron-microscope "Electron microscopes are the most powerful type of microscope, capable of distinguishing even individual ...
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Mites on ginger root

I was just looking at some mouldy ginger found in the fridge under the microscope and was horrified/delighted to find (what looked to be) mites growing in it. I have searched the web and can only find ...
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5 votes
1 answer
739 views

Software for counting fly eggs

Is there any software that could be used to rapidly (quicker than humans) count the eggs laid by Drosophila on a substrate by placing them under a microscope with a camera attached? The eggs are laid ...
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Isotropy of sarcomere bands in skeletal muscle cells

As seen under a polarization microscope, the A-Band in skeletal muscle fibres is so named because it is anisotropic in its refractive index which is a characteristic of orderly crystalline structure. ...
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What are these structures in blood from a nosebleed?

I decided to look at some blood from a nosebleed (not mine) under the microscope and took some pictures of the results. There many large orange structures interspersed within the blood cells and I'm ...
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4 votes
3 answers
15k views

Cannot see images beyond a certain magnification with a microscope

Using a new microscope with my son, we cannot see images beyond a certain magnification: ...
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4 votes
2 answers
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What kind of microscope should be used to view biological structures (such as spores) roughly 5 µm in length?

I would like to be able to identify genera of fungi based on the shape, size, and colour of spores. So the requirements are: Must be able to view a 5 micrometre object clearly with reasonable ...
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4 votes
2 answers
367 views

Atomic force microscopy: depth penetration?

What depth penetration can one typically get with atomic force microscopes? (i.e. how far into the tissue can one image?). I have found numbers on acquisition times (minutes) and spatial resolutions (...
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4 votes
2 answers
1k views

How to operate a microtome?

In the picture above (Source: Amazon.com, United Scientific MCT001 Hand Microtome) we can see a microtome. How is it operated?
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1 answer
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Tissue identification in vascular bundle sections

I always have trouble differentiating the tissues in a vascular bundle. I specially cannot properly identify the vascular cambium and phloem. I know xylem vessel elements are large cells with thicker ...
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4 votes
1 answer
243 views

How can I reduce the background gradient in phase contrast microscopy?

I'm looking for ways to set up a phase contrast microscope to reduce the brightness gradient that appears across the background of my images. I use phase contrast to find the outlines of cells in my ...
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4 votes
1 answer
390 views

How to evaluate the efficacy of an antibody for fluorescence microscopy?

I'm trying to evaluate data taken from fluorescence microscopy with antibody staining, and am wondering whether there is any standard way to evaluate the specificity of the antibody for such ...
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2 answers
106 views

Antibody staining

I am staining tissue sections and I did a mistake, I was supposed to have mixed 3 primary antibodies but I stained only with one of them. After the 1h incubation I washed 10 min with PBS and then I ...
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3 answers
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What is the best way to clean immersion oil from the microscope lens?

My job is to photograph mushroom spores at x100 using immersion oil. What is the best practice for cleaning the oil from the lens? Right now I am just wiping it with a cotton swab.
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4 votes
1 answer
294 views

What microscope/magnification would I need to observe P. Acnes bacteria?

I am currently attempting to grow a culture of P. Acnes bacteria. Right now, my only hope in identifying colonies of the bacteria in the culture is to use a black light to find colonies that glow ...
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4 votes
2 answers
169 views

Does cheese contain cells of animal stomachs?

When cheese is made with calf rennet, is it possible that the cheese contains cells of this calf (stomach)? Dried and cleaned stomachs of young calves are sliced into small pieces and then put into ...
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4 votes
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How to visualize the ECM?

Specifically, I'd like to look at changes in HA (hyaluronic acid) production. Most often you only see people staining the cell surface or removing cells from culture for fixation and then imaging. ...
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4 votes
1 answer
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What is a simple protocol for staining cells in suspension?

I am an engineering student studying how electric fields affect cells, specifically the phenomena of electroporation in living cells. I know that electroporation is widely used for introducing genes ...
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4 votes
0 answers
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How to select a microscope for deep microscopy? Specifically for Deep Learning/ML model development

I am new to biology and microscopy. I am a data scientist. I am faced with a question on selecting a good microscope that enables automation of scanning the slides. And cellular object detection using ...
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4 votes
0 answers
48 views

Can I just simply exchange the eyepiece in a microscope?

I hope it's the right place to ask about microscopy. So my question is what parameters do I have to take into account when buying a new eyepiece for my microscope? Currently I own an Aomekie XSP-42 ...
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4 votes
0 answers
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How can I keep pond water "alive"?

I've been preparing wet slides for my kids to look at from pond water. I have a mason jar of pond water. What can I do to keep the microbes in the jar alive going forward so that we can look at it any ...
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3 votes
5 answers
127 views

Woodland outdoor microscopy advice

Let me start by saying I have never used a microscope outdoors, and in general I have never owned a microscope nor used one regularly. However, I am currently developing and organising some ...
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3 votes
1 answer
63 views

What do the text annotations in an electron micrograph mean?

I saw a photograph taken from an electron microscope, with some text. I want to know what is the meaning of these numbers and letters? Thank you! Regards.
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3 votes
1 answer
3k views

What does fluorogenic mean for fluorescent probes?

Many fluorescent probes are described as being fluorogeninc as an advantage for fluorescence microscopy but what does fluorogenic mean? Dictionary definitions state that it means generating ...
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3 votes
1 answer
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How are new staining protocols designed for microscopy?

How are new staining protocols designed for microscopy? Is it manual work or does it rely on computer simulations? Say for example I thought the H&E staining does not bring enough contrast. How ...
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3 votes
1 answer
535 views

Needle-like crystals observed in wild-grape wine under microscope. Potentially harmful?

I recently crushed and strained about thirty pounds of wild grapes to ferment into wine. About a month in, I did a visual inspection of the wine under microscope to try and identify some yeast and ...
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3 votes
2 answers
206 views

How much are phase-contrast and dark-field microscopy used in biological research? [closed]

How much are phase-contrast and dark-field microscopy used in biological research today? It seems these were invented a long time ago, so I am thinking would it be a good idea to learn these ...
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3 votes
1 answer
588 views

Benefits of CLARITY?

What are the benefits of CLARITY over this technique that was published more than a year earlier? Of course the second technique needs a fancier microscope that is likely more expensive and requires ...
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3 votes
1 answer
141 views

Sample suggestions for phase contrast microscopy

I am a non-bio student working on an academic paper recently and would like to have some sample preparation for phase contrast microscope. For it to work, I imagine the sample to be: Transparent; ...
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3 votes
1 answer
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Can oil be used instead of water in preparing wet mounts for microscopy?

Is there a reason that oil (e.g., vegetable or clear mineral) is not used to make wet mounts in microscopy? I am not referring to oil immersion technique but substituting oil for water in wet mounts ...
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3 votes
1 answer
485 views

What are these stars in my microscopic image?

I see this very curious patterns on the sun-facing side of a jasmine (probably) leaf - visible with a makeshift microscope (about 50x) What are them? Pores? Are there other plants with similar ...
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3 votes
1 answer
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Ferns: How big can a circular sorus be?

I looked at some small ferns under the microscope. The circular sori of the ferns I looked at were less than 2 mm in diameter. The microscope I used had rather weak magnification, so I didn't get to ...
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