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Questions tagged [molecular-biology]

The study of the molecular processes underlying life.

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Human Body Electricity

Since the human body has electricity, can the human body use that energy in any possible way? Such as using it to power something in any way. As you know we can use technology because we have ...
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1answer
10 views

How to confirm secondary structure formation of Precursor miRNA on gel?

I want to fold precursor-miRNA into its secondary structure and then confirm it on gel. At first, I heat it in annealing buffer at 95 C for minutes and cool down slowly. Then I run it on gel but the ...
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2answers
8k views

The GUG start codon in E. coli: identity of initiating tRNA and efficiency of translation

Translation in E. coli is usually initiated at an AUG codon, which encodes the amino acid methionine. In some cases, however, the start codon is GUG, which normally encodes valine. If GUG is used as ...
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0answers
10 views

How does hydrophilic and/or hydrophobic components of polysaccharides influence their 3 dimensional conformation?

It is my understanding that polysaccharides are insoluble in water. Does this mean they are hydrophobic? Beyond being insoluble in water how does this affect their 3D structure? Thanks!
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1answer
108 views

Is one Short Tandem Repeat (STR) enough for a reliable identification?

The lab simulates the analysis of just one STR in the genome. Would this analysis be sufficient for a reliable identification in real life? If not, explain what could be done using DNA to identify ...
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0answers
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How does CRISPR/Cas9 compare to plasmid injection for editing new genes into cells?

I've heard that both of these techniques can be used to add new genes to cells, so I'm curious as to how they compare, e.g.: can plasmids be used in eukaryotes like CRISPR? are plasmids kept after ...
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0answers
10 views

do platelets cytoplasm contains DNA?

I'm a 12th-grade student and I have an entrance exam this year. I have a question in biology. I would appreciate you if you help me <3 as we thoroughly know platelets are multiple pieces of a big ...
2
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0answers
31 views

RNA Molecule folding

For a Single Stranded RNA Molecule : A) Adenine Content Always Equals to Thymine Content B) Cytosine content Equals to Guanine content C) Adenine content Always Equal to Uracil D) Guanine content ...
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0answers
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How is Saccharomyces cerevisiae grown on acetate and ammonium able to produce TCA intermediates?

I am working with a metabolic model of Saccharomyces cerevisiae and I am studying its growth on acetate and ammonia. I am performing Flux Balance Analysis to compute the growth rate and then I am ...
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1answer
17 views

Size constraints on CRISPR guide RNA

I had a quick questions on the size limitations of a CRISPR guide. More specifically on the shorter end. Can I make a guide that is say 7-10bp and still have an active complex? I transfect using an ...
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0answers
16 views

How to rid MRSA on my lower calves that have been getting worse for over a year now [closed]

MRSA in lower right extremity and partial MRSA in lower left extremity/calves/Edema related from lung cancer operation 4 years prior.
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3answers
171 views
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1answer
2k views

Can bacteria, viruses, and cancer cells be destroyed by resonance?

Radiotherapy has been used to treat cancer. Can the resonances by coordinated electromagnetic waves (and/or other forms of waves), of various frequencies, amplitudes and pulse rates, directed from ...
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1answer
30 views

Improving DNA quality and yield from stool samples

I prepared genomic DNA from pig stools but the concentration was very low — about 3~4 ng/µl, when I measured it using nanodrop. The A260/A280 and ...
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1answer
25 views

Why pellet and resuspend E. coli for plasmid prep

For maxipreps, cant you just add all the stuff that would be in P1 (RNase A, EDTA) then just add P2? Because if we pellet cells then resuspend theres got to be a reason, right? Is it slats and other ...
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1answer
19 views

Is the lac operon repressed in the presence of both glucose and lactose?

In the presence of both sugars (glucose and lactose) will there be repression of the lac operon completely? I know that more glucose means less cAMP --> less CAP --> less positive regulation, and ...
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1answer
25 views

Could deutrium oxide be used to make organic compounds with Deutrium instead of hydrogen? [closed]

I am wondering for prokaryotes if Deutrium Oxide could be made into Deutrium Glucose or other biological compounds with Deutrium.
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3answers
8k views

Do we consume dna, proteins of other organisms?

When we eat raw meat, e.g. chicken or fish, we are actually consuming the DNA, proteins etc. which are present in their cells. Wouldn't this affect our cell functions as this DNA might enter our ...
0
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1answer
81 views

How are single-stranded binding proteins removed from the lagging strand during DNA replication?

The lagging strand, downstream of the Okazaki fragment, is covered in single-stranded binding proteins (SSBPs) during DNA replication. What is the mechanism which ensures that SSBPs are removed from ...
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1answer
25 views

Design rules for DNA linkers

I want to use double stranded DNA linkers to physically bind two "things" together, by grafting ssDNA on each one of them and using DNA hybridization as the locking mechanism. I do not expect the ...
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1answer
27 views

Ligation without purifying insert

I am planning to insert a 45 bp sequence in a vector. After restricting my insert to create compatible sticky ends, I am finding no way to clean it up. Is there any way for cleaning this fragment ...
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1answer
20 views

How to identify whether something is in linkage disequilibrium?

If the following loci indicated the presence of an SNP in flu strains, is Locus 2 and Locus 3, which are located 10 bp apart in linkage disequilibrium? "When alleles and molecular markers are ...
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0answers
11 views

Why are higher doses of atropine required to produce central effects?

Reason given in my book is restricted entry into the brain..is it something to do with the chemisty?
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1answer
29 views

What is the difference between a signal peptide and a transit peptide?

From what I know, the two names are used interchangeably and I haven't found any resource which says otherwise either. Is there at all any difference, is there a transit peptide that is not a signal ...
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2answers
652 views

How will changing the concentration of a Tris buffer affect amylase enzyme activity?

For instance if you increase the amount of Tris but pH still does not change then will the enzyme activity still proceed normally? If it does change the pH will it change enzyme structure and why?
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1answer
26 views

Will DNA nanostructures be useful for medical applications?

There is a lot of work being done on developing small nanomechanical structures and even small nanomechanical actuators built with DNA. I have heard researchers motivate this research with claims ...
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1answer
16 views

What is an acceptable qPCR efficiency when adjust using the Pfaffl equation?

I have efficiencies of 76% and 85%. THis is clearly below what is normally acceptable. But the pfaffl equation corrects this, doesnt it? However is it acceptable to publish this data?
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1answer
52 views

Gene vs Protein homology

I am trying to find homologs for some proteins and I am wondering if it is better to find it by comparing the proteins or the coding genes or either is fine.
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3answers
228 views

Problems with housekeeping genes

I have tried several housekeeping genes to analyze the relative expression of a cytokine for measure the inflammatory local response in mice ears, all the housekeeping genes I have tried are not ...
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1answer
21 views

Why do we use different micropipette tips for different volumes? [closed]

Isn’t it easier to use a 1000 µl micropipette tip for all volumes? I don’t understand why there are different tips for smaller volumes. Does it affect the accuracy of the pipette for very small (<...
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1answer
28 views

Melting point of animal fat and plant fat

I notice in daily life that animal fat typically has a higher melting point than plant fat does. I wonder the reason why this happens. What exactly contributes to this difference? Is it because of the ...
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1answer
37 views

Why are there inverted repeats in response elements?

The following picture shows an inverted repeat sequence in a response element. Response element sequences for glucocorticoids, estrogen, and thyroid hormone show that they all contain inverse repeats. ...
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0answers
15 views

Formation of annular structures in carbohydrates

In Lehninger's Principles of Biochemistry, David L. Nelson and Michael M. Cox state that monosoccharides with more than four carbon atoms often have cyclic structures (pyrannoses and furans) and ...
7
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1answer
161 views

Does depolymerisation take place at the minus end of microtubule?

Wikipedia says that Dynamic instability refers to the coexistence of assembly and disassembly at the 'ends' of a microtubule. but Karp's Cell Biology, 7th edition says Dynamic instability is ...
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1answer
37 views

When muscles contract is the process similar to how non-newtonian fluids react?

I'm trying to understand how muscles contraction/tension works but getting loss in on the cellular level. From my understanding, when muscle tissue need to contract, the cells are flooded with calcium?...
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1answer
74 views

Why replication collapse but not stall leads to DNA break?

I have been looking into the concept of replication dynamics and was wondering why collapsing but not stalling leads to a DNA break.
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1answer
75 views

Is there a reliable source for storage and stability of reducing agents like DTT?

Reading the literature on DTT, one is confronted with a confusing mass of papers; some claim that a 1M solution in water is stable, other papers say it is not. I use the reaction with DTNB to show ...
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0answers
18 views

How to take precise/low number of cells from cell lysate? [closed]

I would like to know that how can we take low or precise number of cells from a cell lysate? (without using any protein quantification assay). Let say, If I have a cell line having cell density 5x10^...
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1answer
20 views

Does *E. coli Dh5 Alpha* strain has the ability to degrade polyethylene?

Does E. coli Dh5 Alpha strain has the ability to degrade polyethylene? What are the bacteria that can be used as a control in the research of polyethylene degradation?
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0answers
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Multiplex real-time PCR on different sized amplicons

I'm planning to do multiplexing on my qPCRs for 3 different amplicons with widely varying sizes (180 bp, 200 bp and 370 bp) Is there going to be a significant bias in the quantification due to the ...
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1answer
40 views

Antigen molecular mimicry

Let us consider a situation in which the body is attacked by a microbe, and the microbe is captured by the immune system for recognition of surface antigens. The surface antigen recognized mimics one ...
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0answers
32 views

What M9 stands for

Can anyone tell me what M9 stands for in M9 medium? I have tried googling but I still cannot find it.
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3answers
182 views

Retrieve all predicted cds from NCBI

Please apologies if this has been answered somewhere else, but I couldn't find an answer to this problem. I would like to retrieve all the predicted coding sequences on the NCBI ftp for a given ...
5
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1answer
506 views

Are all genes transcribed in differentiated cells?

My textbook tells me that it’s specific transcription factors that allow for a different set of genes to be expressed in different cells (differential gene expression). My book gives the example of ...
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2answers
158 views

number of chromatids seen in karyotype

The karyotype is performed on a cell whose cycle has been stopped in metaphase or pro metaphase, using colchicine or by other means. In the textbooks I read that during the S phase each of the 46 ...
0
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1answer
23 views

Insertion Confirmation Cloning Strategy

So I am in a bit of a time constraint. Essentially, I inserted a DNA fragment via molecular cloning which contains a unique RE site. I need to confirm whether my colony has or does not have the ...
2
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1answer
30 views

Is studying chemical and physical properties of chemical substances that make up organisms really a task of molecular biology?

I have read in a high school textbook that (translated into English by myself): "Branch of science that concerns itself with studying chemical and physical properties of substances that make up ...
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2answers
90 views

How can you band wheat chromosomes using Giemsa dye?

I am working with wheat chromosomes and trying to stain the chromosomes. However, during staining with Giemsa dye chromosomes appear totally dark. I'm not sure why this might be. Anyone have any ideas?...
2
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1answer
48 views

Why is Turner's syndrome a problem?

So Turner's syndrome is a chromosomal defect where one of the X chromosomes is missing. For more details visit the [Wikipedia page] (https://en.m.wikipedia.org/wiki/Turner_syndrome). But I don't ...
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0answers
19 views

Can we spike with a different enzyme to a SYBR Green Master Mix?

I followed the standard SYBR Green Protocol for doing a qPCR. For which I used 10 uL of 1X SYBR Green Master Mix Forward Primer and Reverse Primer (each at a final conc. = 8.5 uM) Template (unknown ...