Stack Exchange Network

Stack Exchange network consists of 174 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

Visit Stack Exchange

The scientific study of the structure and function of genes at the molecular level, particularly chromosomes and DNA.

0
votes
1answer
15 views

Insertion Confirmation Cloning Strategy

So I am in a bit of a time constraint. Essentially, I inserted a DNA fragment via molecular cloning which contains a unique RE site. I need to confirm whether my colony has or does not have the ...
17
votes
3answers
3k views

Are mutations a source of genetic variation?

Here is a question from the book SAT II Success Biology E/M (where the SAT is the exam taken by the American high school students): Which of the following statements is true about mutations? (A) ...
9
votes
3answers
450 views

Why did scientists think humans had 100,000 genes (before the Human Genome Project)?

One of the major results of the Human Genome Project (HGP) was that humans have far fewer separate genes than previously thought. From a 2004 article about the HGP: Francis S. Collins, director of ...
0
votes
0answers
5 views

Ratio PD:NPD:TT in tetrad analysis

In tetrad anlysis in yeast it is often used that if two genes are unlinked and far from centromere, the expected PD:NPD:TT ratio is 1:1:4, i.e. we are getting PD and NPD each with probablity 1/6 and ...
0
votes
0answers
10 views

Meting temperature of PCR products and product expected size

I know the Melting temperature of my primers but I would like to know if the melting temperature of my product match with the melting temperature I get in the melting curve when doing a qPCR, in order ...
1
vote
1answer
25 views

What does “novel, predicted deleterious, within autozygome” mean in genetics?

I am a medical student and just got involved with a genetic lab and research. I read a lot phrases like: "Although no mutations have been reported in this gene, we think this variant is interesting ...
0
votes
0answers
25 views

Immortal DNA Hypothesis

Confused by the immortal DNA hypothesis. My instructor states that it arises from the notion that stem cells divide asymmetrically, and so I thought that the original DNA template strands were always ...
-1
votes
1answer
60 views

Is it theoretically possible to safely eliminate most viruses in the atmosphere, hence preemptively cure all the viral diseases? [closed]

Could we create a genetically modified virus or bacteria (with inability to mutate into something dangerous for animals) that would quickly spread all over the planet and selectively kill most of the ...
0
votes
0answers
9 views

EJ138 bladder cancer cell line

My question is about EJ138 bladder cancer cell line. Is there any mutation in this cell line, esp in TP53 gene?
0
votes
1answer
20 views

Thousands of Reporters Integrated in Parallel (TRIP) barcodes

Has anyone worked with constructing barcodes for TRIP? How does constructing of barcodes work in general? I don't seem to be able to grasp the basics of constructing these barcodes.
0
votes
0answers
12 views

Why are okazaki fragments of eukaryotes smaller? [duplicate]

Prokaryotes have bigger fragments as compared to eukaryotes. Why so ?
4
votes
2answers
136 views

How does transcription end?

In rho-dependent termination in prokaryotes, how does RNA polymerase “know” that it has reached the end of a gene and that it has to stop so that the rho-factor can bind mRNA’s rut site? Is there a ...
1
vote
0answers
18 views

Young family members to learn genetics - Amino Labs? [closed]

I'm an engineer but want to learn about biology with a niece and nephew who are early teens. I saw Amino Labs (https://amino.bio/) and thought because I work a lot with my hands, doing hands on ...
1
vote
1answer
25 views

How do PrP mutations lead to prion disease?

My understanding is: The PrP gene in human cells is expressed as both PrP-c (normal protein) and PrP-sc (prion disease protein). This happens post transcriptionally, that is, the normal and the ...
1
vote
1answer
23 views

What is the role of CRISPR-dCas9 in gRNA-dCas9 transcription regulator complexes?

In this paper, I read that mutant versions of Cas proteins such as a deactivated Cas9 (dCas9) are used alongside a guide-RNA (gRNA) to form variants of CRISPR tool that can function as transcription ...
0
votes
2answers
91 views

Can a viable embryo develop from the fusion of two egg cells?

For a zygote to form, two haploid gametes undergo meiosis and fuse during fertilisation. Since two egg cells (or even two sperm cells) are both haploid, is it theoretically possible for them to make ...
5
votes
1answer
99 views

How does DNA codon (5`-3`) convert to mRNA?

There is a problem I stumbled upon when doing a lesson on molecular genetics (Grade 12 level). The lesson contains many tables in this format: I don't understand how it got to the mRNA codon ...
1
vote
1answer
68 views

About The Selfish Gene Book - How Replicators Molecules forms duplicates?

I was reading The Selfish Gene. In the 2nd chapter - "Replicators" I read: Think of the replicator as a mold or template. Imagine it as a large molecule consisting of a complex chain of various ...
2
votes
0answers
55 views

True examples of common variation due to Mendelian Inheritance

Classic examples of mendelian inheritance are genetic diseases such as sickle-cell anemia, Tay-Sachs, cystic fibrosis, and xeroderma pigmentosa. For some of these diseases, it is believed that they ...
0
votes
0answers
19 views

How to calculate the Marker Index(MI) for a dominant marker such as RAPD and ISSR?

I am working in the genetic diversity analysis of a plant species and have used RAPD and ISSR markers. After scoring the data in a binary matrix, I am trying to find the marker index of the primers. I ...
2
votes
2answers
50 views

What dictates which protein to synthesize at a given time?

I understand how DNA is replicated and how it directs the synthesis of proteins from amino acids through RNA polymerase, RNA and ribosomes. Now I want to understand how it works in the big picture. ...
0
votes
0answers
12 views

Question Concerning Plasmid Transplant

Upon reading a report today, I discovered a interesting technic of plasmid transformation. Triparental Conjugation, a bacterial conjugation including three parts , a conjugative plasmid, a donor cell ,...
0
votes
0answers
10 views

Increasing my PCR efficiency to be able to better visualise my bands

I run an electrophoresis in agarose gel and my expected bands are extremely week, is even hard to see them. It looks I need to increase my PCR efficiency. I have read about a Hot start setp, which ...
-1
votes
2answers
66 views

Do all retroviruses evolve into dna viruses? [closed]

Since even nonprocreative sex transfers genetic material it seems redundant for viruses to destroy the host. Retroviruses are sufficiently simple to fit into DNA. So why not just become DNA viruses?
1
vote
0answers
31 views

Isolation of Intact Granules from Mast Cells

How to isolate intact granules from mast cells without using sucrose and percoll?
0
votes
1answer
57 views

Is it Theoretically Possible to Create a Male clone from the donor Cell of a Female?

I understand that Dolly the Sheep was created without the presence of a sperm cell. Instead, nucleus DNA from somatic cells of adult sheep were used. Since Dolly was female, i am assuming that the ...
0
votes
0answers
10 views

primer blast for BSP methylation

How can I blast my BSP(Bisulfite sequencing) primers? I designed in this way that C nucleotide converted to T and my primers just sit on the converted DNA! I blast it with Bisearch but I don't know ...
1
vote
1answer
13 views

Is there a biological explanation for a 0.5 difference in allele size with PCR product?

CONTEXT I am currently working on a set of diversity, this diversity in interspecific (within the same genus). I am using SSR markers, the primers were designed on one species and are working really ...
2
votes
0answers
32 views

how can I get stronger staining for my lymph node sections

I am using the same protocol and same antibodies that the literature says but still I cannot get good staining for my lymph node sections, I tried to change the fixation method and I am using now ...
1
vote
0answers
32 views

Control for Bisulfite sequencing

I am wondering how can I control my converted DNA! I converted my DNA with bisulfite then I amplified the converted DNA with specific primers of BSP and purified my product and finally sequenced the ...
2
votes
1answer
26 views

Expected bands in polyacrylamide gel

I have something in my notes that doesn't seem right. If I use restriction enzymes on a PCR product how many bands should I see provided I know the restriction enzimes will cut the DNA? If I use two ...
2
votes
1answer
43 views

Antibiotic resistance

I have a biological puzzle that's been perplexing me for years. Can you review my logic and tell me where I'm wrong or if I'm on to something. As I understand it, most, if not all genetic ...
0
votes
1answer
32 views

How do I reverse my insert within a plasmid?

I have a plasmid with: ...T7pro. -- RBS -- XBa1 cut site -- ProDpro. -- RBS -- AmilCP -- T -- Spe1 cut site -- T... How do I design primers to reverse the portion in bold, such that the T7 and ProD ...
0
votes
1answer
36 views

What are the types of interactions in biological network (protein networks)?

In the KGML files, the types of relations between genes or proteins are precisely activation, inhibition, expression, repression, indirect effect, state change, binding/association, dissociation, ...
0
votes
2answers
64 views

How were genes located before the development of bioinformatics?

I would like to know the detailed procedure of how scientists in earlier time were able to locate genes like how we were able to locate Huntington gene in 1983?
1
vote
1answer
45 views

Why are not both alleles knocked out at the same time in knock out mouse?

I was reading this little 'article' about homologous recombination and knockout mouse. According to this article you first remove embryonic stem cells from a gray-fur blastocyst, then insert the ...
0
votes
0answers
12 views

Is there a distinction between linkage phase and haplotype?

Linkage phase- combination of alleles that are coinherited Haplotype- combination of alleles an organism has at different loci. So could an informative meiosis be defined as a meiosis in which a ...
1
vote
1answer
51 views

What is the difference between alarmones and riboswitches

An Alarmone is an intracellular signal molecule that is produced due to harsh environmental factors. They regulate the gene expression at transcription level. A riboswitch is a regulatory segment of a ...
0
votes
0answers
42 views

What are the interactions between mtDNA and nucelus' DNA?

In an exam situation I have been presented with the following question by the teacher: What are the interactions between the mitochondrial DNA and the cell's nuclear DNA? To my knowledge there is ...
0
votes
0answers
17 views

Will the addition of 3 amino acids in the C-terminal of a membrane protein affects its properties?

I am planning to use 2A self-cleavage peptide to express my gene of interest downstream of an intrinsic gene in my model organism so that I can exploit the intrinsic promoter, instead of cloning it ...
0
votes
0answers
13 views

Why may some telomeres not appear on metaphase chromosomes using Q-FISH?

There are some chromosomes that appear to have missing telomeres e.g. 2 instead of the expected 4, but there appears to be no fusions or breaks.
1
vote
0answers
17 views

What is the chemical mechanism by which PcP(c) is converted to PcP(Sc) in Fatal Familial Insomnia

I know the gene PRNP and it's location. There’s a mutation at codon 178 (asparagine found instead of normal aspartic acid) on the prion protein gene (also found in fCJD) and more uniquely there is a ...
4
votes
1answer
40 views

Reversal of cross-linking in ChIP-seq

Chromatin immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell. A summary of the protocol for ...
0
votes
1answer
86 views

What are the advantages of using interphase cells rather than metaphase cells for gene mapping purposes using FISH?

I have been told that interphase cells are better for gene mapping purposes than metaphase cells but don't know why.
3
votes
1answer
55 views

Do tRNAs that recognize multiple codons have any preference for one over another?

What are the effects of the different binding strength/affinity between the synonymous codons corresponding to a single tRNA ?
0
votes
0answers
13 views

Looking for neuronal gene expression database

I am looking to see if there is a database that contains gene expression profiles differences of differentially stimulated neurons.
0
votes
1answer
50 views

Can I use commercial DNA loading dye for my RNA samples without degrading them?

I was wondering if I have to use special RNA loading dye or can I run RNA samples with regular loading dye that is used for DNA?
2
votes
1answer
53 views

How to attain a deep conceptual grasp of key atomic/molecular/genetic/ notions? [closed]

It's a total noob question, I know. I'm a layperson trying to come to grips with basic scientific terminology. I always face a conceptual hurdle in trying to understand micro-talk. The way I'm using ...
0
votes
0answers
16 views

Do all E. coli Express OmpC Membrane-Protein?

In a lot of papers I am reading, people are amplifying the OmpC gene (which codes for the OmpC membrane protein), from 'E. coli' but they do not specify the strain they used. According to my ...
3
votes
2answers
48 views

Gene terminology - is one gene a concrete, single physical sequence?

Suppose you have two identical copies of the same, coding nucleotide sequence (e.g. two copies of BCL2 - a random gene I found on Wikipedia). Could you say that these are two genes (i.e. the name "...