Questions tagged [noncoding-rna]
The noncoding-rna tag has no usage guidance.
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How many cuts are done during CRISPR-Cas9 in one cell?
In a CRISPR-Cas9 experiment, the protein cuts the site matching the cRNA part of the gRNA. My question is: How many cuts are possible if multiples sites matching the cRNA are found in the cell?
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How frequent are transposons in action? [closed]
A transposable element (TE, transposon, or jumping gene) is a DNA sequence that can change its position within a genome, sometimes creating or reversing mutations and altering the cell's genetic ...
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Difference between LIGR-seq and PARIS methods
I would like to reuse a dataset produced by LIGR-seq for ncRNA secondary structure prediction in the style of PARIS method.
At ...
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Where in the cell does this enhancer RNA knockdown take place?
In Pnueli et al., 2015, they knock down an enhancer RNA using RNAi, testing whether it is a mere byproduct or whether it has a key role in the enhancer's function. They find their system works: the ...
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Are pseudogene counted as a subtype of long non-coding RNA genes?
Are pseudogenes regarded as a subtype of the genes for long non-coding RNAs?
I could not find the answer to this question in an internet search.
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Why does a cell need siRNA?
My question is about small non-coding RNAs. miRNA and siRNA are both known for mRNA regulation. miRNA can both transcriptionally inhibit and degrade mRNA by cutting it. For inhibition just a small ...
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What is the difference between alarmones and riboswitches
An Alarmone is an intracellular signal molecule that is produced due to harsh environmental factors. They regulate the gene expression at transcription level. A riboswitch is a regulatory segment of a ...
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Eukaryotic equivalent of bacterial tmRNA
According to this Wikipedia article,
tmRNA is only found in bacteria, with its purpose being to “rescue stalled ribosomes”.
This brings me to the question of is there a eukaryotic equivalent of this ...
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Is spliceosomal RNA called snRNP or snRNA? [closed]
I have been reading articles by various sources, and they seem to interchangeably call the U1/U2/U4/U6 compounds involved in RNA splicing snRNPs as well as snRNAs.
This confuses me deeply. They must ...
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Why is Mus Musculus a good model organism? [closed]
I'm reading through a number of articles that use partial hepatectomy in Mus Musculus to study the mechanisms for liver regeneration (1, 2, 3). I have no doubt that a better understanding of ...
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Where does tracrRNA comes from? [closed]
I'm talking about CRISPR system. I know the crRNA is transcribed from the palindromic repeat and the "spacer" but I don't know where the tracrRNA comes from.
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Which are the best programs to analyze circular RNA?
Hello I'm just started with bioinformatics and I would like to know which programs are best for the task of finding circRNA (circular RNA) in sequences of tissues and different cellular types. I have ...
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non-coding RNA modelling in silico
I've been trying to model a non-coding RNA molecule which binds to a RNA binding protein. I found the DNA sequence for ncRNA from NCBI. I did not find the RNA seq of the same. Hence my doubt is, ...
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Are there parts of DNA that do not have genes?
A question in my genetics class is "Are the number of genes proportional to the genome size?"
The answer is "no," and is it because the genome is the entirety of all the DNA in an organism, but that ...
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Can ncRNA convert to coding RNA/mRNA by mutations?
DNA has transcribed into RNA (Non-coding). Can this RNA mutate and become a Protein-Coding one/mRNA? Have there been any such instances reported by scientists?
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What is meant by "Expression" of Non-Coding RNA?
I was having a look at lncRNAdb and its help says:
The ENCODE project gene annotation list, GENCODE, has predicted that the human genome contains 14,470 lncRNAs whereas only a small proportion of ...
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Small non-coding RNA (sRNA) vs. micro RNA (miRNA)?
What are the differences between micro RNAs (miRNA) and small non-coding RNAs (sRNA)? Are these two terms used interchangeably?
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Are non-coding RNAs introns?
I am slightly confused as to what part of the genome codes for non-coding RNAs. Is it the introns? This would make sense to me as to why they are not transcribed as the introns are not transcribed. Or ...
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Are some non-coding RNA spliced?
Are some non-coding RNA spliced like mRNA? I tried to find some information but I don't find anything...
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