Questions tagged [ph]

pH is a measure of the acidity of a solution. It is defined as the negative logarithm (base 10) of the concentration of hydronium ions in a solution.

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1answer
8k views

Why do you die if you cannot breathe?

I was wondering what the actual reason for death by suffocation is. Obviously it is related to oxygen deprivation. But what is the underlying cause of death? Is it due to insufficient oxygen for ...
3
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1answer
4k views

What are the highest and lowest possible pH, paCO2 and HCO3 in the blood of living human?

What are the highest and lowest possible values of pH, $paCO_2$, and $HCO_3$ in the (arterial or venous) blood of a living human being?
7
votes
1answer
30k views

Determining net charge of amino acid at given pH

I am trying to calculate the net charge on an amino acid, but I seem to be doing it wrong. The question I am trying to answer is "In a pH 7.0 buffer, what will be the charge of Methionine?" We are ...
5
votes
1answer
5k views

Does pH affect Michaelis constant?

I have been trying to confirm the Km of a substrate (which is 34 +/- 4 mM). This value was obtained in 50 mM MOPS, pH 6.3. I conducted my kinetics assay in a buffer of pH 7 and obtained a Km value in ...
5
votes
3answers
59k views

Why does pH have an effect on enzymes?

I am currently studying biology and would like to know why enzymes work best in a particular narrow range of pH (the so-called pH optimum). Unlike temperature change, I do not think this has much to ...