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Tagged with plasmids bioinformatics
4 questions
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vote
0
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Plasmid harbouring subunit S of type I R/M system
We have an assembly of a genome of a Lactobacillus species. The genome also contains a 7.5kb plasmid.
Aside from the "usual plasmid genes" the plasmid contains:
- toxin/antitoxin system
- Subunit S ...
- 195
1
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1
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Reading annotations from a plasmid map
Can anyone explain why inside plasmid mapping software the annotations are layered under each other, I am confused as to why there are essentially two layers within the map, for example in the image ...
- 137
3
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4
answers
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Identifying the origin of replication of an unannotated *E. coli* plasmid
I have attempted a few searches for a list of origins of replication for plasmids in E. coli, but I was only able to find a list of origins, but not their individual sequences. The available plasmid ...
- 9,462
2
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2
answers
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Primer design for Gibson assembly
I'm trying to design a primer for Gibson assembly. My gene of interest is on a plasmid, and I want to copy that gene, and put it into a different plasmid.
I am unsure how to design my primers for ...
