All Questions
Tagged with plasmids recombination
5 questions
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Why does plasmid recombination require precise "copying and pasting" but microinjection doesn't?
Just learning about biotechnological techniques of gene transfer at the moment. With bacterial plasmid recombination, from a high-school level, we are taught that:
The desired gene is cut using a ...
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Why is Homologous Recombination (HR) more frequent at long sequence repeats?
I'm studying plasmids in bacteria (E. coli), and trying to understand the well-cited phenomenon that recombination frequency increases with longer repetitive sequences. I think this also applies to ...
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Join a linear plasmid just using a primer (without ligase enzyme)
Can I use just a primer and PCR to join the cutted plasmid like this ? without using any further enzyme like ligase.
After the linear dsDNA plasmid annealed into 2 ssDNA, primer will bind to middle. ...
- 945
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Questions in Regards to Isolating Promotor Region for Recombinant DNA Techniques
For a while now I have been trying to isolate the promotor region of this Protein.
Using sticky end PCR, I need to isolate this promotor region so it has paticular sticky ends for the purpose of ...
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Restriction enzyme digestion
A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B. This produces a 3000 bp and a 2000 bp bands when visualized on an agarose gel. When digested with one ...
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