Questions tagged [prokaryotic-cells]

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Storing bacteria in a dry, room temperature long-term storage whilst maintaining viability

I'm quite new to biology, but I'm wondering if there are any methods of storing bacteria in long-term, dry conditions with no climate control. Perhaps they could be dormant in a powder or granule-like ...
Lee's user avatar
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How can I easily distinguish between eukaryotic and prokaryotic cells using flow cytometry?

I am trying to count populations of cells in a co-culture. One organism is Pichia pastoris and the other organism is a gram-negative bacterium. Pichia stains gram-positive using crystal violet and the ...
Noah Sprent's user avatar
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In what stage of bacterial growth can penicillin inhibit the growth of the cellular wall?

The bacterial growth has 3 main stages: lag-phase, log-phase and stationary phase. I was wondering in which one of them penicillin can inhibit the growth of the cellular wall of bacteria and why. I ...
Francesca's user avatar
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What defines a single organism?

I suppose what prompts me to ask this question is my wondering if it is possible to have a multicellular prokaryotic organism. For instance, can a biofilm be considered a single organism? Why or why ...
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How do I homogenise a small pellet of 10 to 50 cells within less than 50 µL?

I have a problem with declumping/ homogenising bacterial cells in small volumes (10 to 50 µL). I know that I can get all of my cells out of the tube, but they are still stuck together and won't form ...
Sam's user avatar
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1 answer
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DNA replication - how many times and when does it occur?

I’m currently learning about DNA replication in both prokaryotic and eukaryotic cells. And my lecturer has mentioned that replication is a once in a lifetime activity. And I’m not sure what this is ...
Irene Lee's user avatar
13 votes
1 answer
4k views

Do all bacterial cells have a double membrane?

I’m reading that gram positive and negative bacteria have an inner plasma membrane surrounding the cytoplasm, then a periplasmic space, then an outer membrane. How does this compare to my general ...
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Why is translation so much faster in prokaryotes than eukaryotes?

Prokaryotes perform transcription and translation much faster than eukaryotes. If memory serves, a single 70S prokaryotic ribosome can incorporate around 20 amino acids per second, whereas the 80S ...
forest's user avatar
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Are there differences between the activation proteins of Eukaryotes and those of Prokaryotes

I'm in BIO 203 (for reference to my skill level), and I noticed the textbook makes a whole section out of transcriptional activator proteins, their function and applications in eukaryotes, but in ...
PGEchic's user avatar
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1 answer
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How do you differenciate between the eukaryotes and prokaryotes?

I wonder how we differentiate between the eukaryotes and prokaryotes. Eukaryotes: Eukaryotes (also spelled "eucaryotes") comprise animals, plants, and fungi—which are mostly multicellular - as ...
Bobtrollsten's user avatar
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Difference in multicellularity between prokaryotic and eukaryotic organisms

As most prokaryotic organisms are single-celled, and in eukaryotic organisms this is the reverse, is there some evolutionary advantage that led to these features evolving? Or is it purely the fact ...
Elizabeth T's user avatar
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How Mitochondria differs from Prokaryotes(probably regarding energy production)? [closed]

In other words, what evolutionary advantage is offered by mitochondria to eukaryotes(probably regarding energy production), which prokaryotes themselves cannot evolve or do?(here I am assuming that ...
amsquareb's user avatar
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Advantages and disadvantages of sporulation compared with competence in bacteria?

Why do bacteria have both of these mechanisms to deal with the same environmental stress: nutrient deprivation? In a culture exposed to this condition, often both competent cells and sporulated cells ...
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3 votes
1 answer
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Is Sda a protein, or is it a protein domain of DnaA?

I initially thought that a domain was a specific part of a protein, with it given tertiary structure, to which a given molecule is able to bind. (I think I recall phrases such as "the haem binding ...
Meep's user avatar
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1 vote
1 answer
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Do Alicyclobacilli produce carbon dioxide?

Do Alicyclobacilli, especially guaiacol producing ones, still produce carbon dioxide? Alicyclobacillus spp. are well known spoilage organisms in the fruit juice industry. Apparently there is no "...
Johnny's user avatar
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5 votes
1 answer
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Why are plant cells rectangular and animal cells spherical shaped?

Is that because the plant cells have cell walls and animal cells don't, or is there a function performed by the different shapes? Perhaps round shapes assist the movement of cells?
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1 answer
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Why did eukaryotic cells develop? [closed]

If eukaryotic cells can survive in extreme conditions, then why are their still prokaryotic organisms?
Babra Ejaz's user avatar
4 votes
3 answers
21k views

Why do bacteria die in a high sugar content environment?

I was reading some questions in the test bank. Then in Chapter 27, I wonder why the answer is A. (undergo death by plasmolysis). Is the high concentration of sugar that kills them or the effects of ...
Joseph's user avatar
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1 answer
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What is the name of cellular respiration that Prokayotic cells use?

Eukaryotic cells use mitochondria using anaerobic or aerobic respiration. Prokayotic cells lack mitochondria but are bacteria. Mitochondria originated from bacteria and can be used in the same way. ...
anonymous's user avatar
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14 votes
2 answers
41k views

Linear and Circular DNA

So school has taught us that eukaryotes have DNA that is linear and inside of a membrane - called the nucleus. And that prokaryotes have circular DNA that is free floating inside of the cell. We ...
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