Questions tagged [protein-engineering]

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Why are camelid-derived nanobodies called VHH (variable heavy domain of heavy chain)?

Single-domain antibodies (or nanobodies) derived from camelid heavy-chain antibodies are called VHH antibodies, where VHH stands for "variable heavy domain of heavy chain". I assume the ...
mgarort's user avatar
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5 votes
3 answers
202 views

Saturated Mutagenesis Screening

I am hoping to mutate the active site of the enzyme I am researching that has 5 residues in proximity with the substrate. I am wondering how many colonies I'll have to assess to theoretically sample ...
JEJS's user avatar
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Are there any small peptides with protease activity?

I'm looking for a very small protease to base an engineered protein on. I did a search for "peptides with protease activity" but all the results seem to be as peptides to be used as protease ...
Alex I's user avatar
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1 vote
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Papers where protein oligomerization has been engineered away

I have a protein that acts as a monomer, but appears to have a relatively high self-affinity in vivo (measured using transient transfection of a plasmid coding for the protein), which is annoying ...
Mike Flynn's user avatar
1 vote
0 answers
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Young family members to learn genetics - Amino Labs? [closed]

I'm an engineer but want to learn about biology with a niece and nephew who are early teens. I saw Amino Labs (https://amino.bio/) and thought because I work a lot with my hands, doing hands on ...
user97113's user avatar
3 votes
0 answers
75 views

Low cost cell free expression system

I am a graduate student looking to incorporate some protein engineering into my training. I have been reading a lot about E coli lysate based systems, but they still require 3rd party additives like ...
TheCodeNovice's user avatar
3 votes
1 answer
462 views

Could AI be applied to protein folding?

Two years later, there is a follow up question to the one asked here: How do we know if the folding@home project results are right? Since we are quite sure F@H is working right and following this ...
kreuzerkrieg's user avatar
5 votes
1 answer
1k views

what is the meaning of VIP-cre::LSL-TOM::SOMGFP(GIN) mice?

I am reading the following paper and the researchers use "VIP-cre::LSL-TOM::SOM-GFP(GIN)" mice (page 4) for multi cell patch clamp and "VIP-cre::LSL-TOM and SOM-cre::LSL-TOM" mice injected ...
user135172's user avatar
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62 views

Is it feasible to grow edible crops for lawns?

Is it possible to grow some type of plant that functions well as a lawn (e.g. is somewhat grass like), but that is humanly edible? Would anything that currently exists fit this role? For example ...
Jonathan's user avatar
  • 103
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1 answer
57 views

Computational engineering of protein binding pocket

I have an X-ray structure of an enzyme with reported activity to a small molecule. This activity is rather low since it is not the native substrate. I can run molecular modeling simulations (e.g. ...
Dahlai's user avatar
  • 207
5 votes
2 answers
349 views

What is protein folding and how is it relevant to disease?

I am trying to understand what is protein folding and how it could help cure some diseases. When reading articles about it, it looks like the goal is to find perfect folds for proteins because some ...
Pop Flamingo's user avatar
2 votes
2 answers
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Sheep vs. Furry animals

Why can't we take the fur of a fox without killing or skinning it just like the sheep , what's the biological or chemical structure or even the trait which is on a sheep's body that separates wool ...
user27247's user avatar
10 votes
3 answers
684 views

How do we know if the folding@home project results are right?

Some of us are involved in the folding@home project, spending time, money, and resources. I would like to know the answer to two main questions: How do we know we fold it right? I mean, these models ...
kreuzerkrieg's user avatar
0 votes
1 answer
558 views

How far can we go in engineering our own evolution? [closed]

After recently seeing Christopher Nolan's Interstellar and reading Kip Thorne's The Science of Interstellar, I've seriously been reflecting on the challenges mankind faces in becoming space pioneers, ...
docscience's user avatar
1 vote
1 answer
38 views

Could the proteome of E. coli be fluorescently labelled?

What proportion of the total number of proteins in E. coli could be fluorescently labelled for PALM/STORM imaging?
user2002858's user avatar
3 votes
1 answer
49 views

Has any research lab done serious work to engineer new bacteria which assemble graphene wafers?

I was thinking about crazy uses for engineered bacteria. Nano-assembly of Graphene seems like a potentially excellent target for the technology. Have any research/full-blown labs worked on this? Any ...
SmugDoodleBug's user avatar
8 votes
0 answers
734 views

Rosetta ab initio prediction and protein-protein interaction fitness help [closed]

I have designed several proteins which I predict have interactions with another protein using the sequence based Conjoint Triad Method. I would like to know which ones structurally are predicted to ...
24 votes
1 answer
736 views

Phagemid display

If I'm using a bacteriophage for phage display and I'm trying to avoid avidity effects by using a helper phage what would be the best way to maintain a large library size while keeping everything ...
bobthejoe's user avatar
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7 votes
1 answer
609 views

What is the fastest way to build an alanine scanning library?

For interfacial studies, I would like to build an alanine scanning library for one of my proteins examining 20 sites. I will ultimately express the gene using E.coli cell-free protein synthesis. I ...
bobthejoe's user avatar
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