Stack Exchange Network

Stack Exchange network consists of 174 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

Visit Stack Exchange

Questions tagged [protein-engineering]

The tag has no usage guidance.

1
vote
0answers
32 views

Papers where protein oligomerization has been engineered away

I have a protein that acts as a monomer, but appears to have a relatively high self-affinity in vivo (measured using transient transfection of a plasmid coding for the protein), which is annoying ...
1
vote
0answers
19 views

Young family members to learn genetics - Amino Labs? [closed]

I'm an engineer but want to learn about biology with a niece and nephew who are early teens. I saw Amino Labs (https://amino.bio/) and thought because I work a lot with my hands, doing hands on ...
3
votes
0answers
32 views

Low cost cell free expression system

I am a graduate student looking to incorporate some protein engineering into my training. I have been reading a lot about E coli lysate based systems, but they still require 3rd party additives like ...
2
votes
1answer
419 views

Could AI be applied to protein folding?

Two years later, there is a follow up question to the one asked here: How do we know if the folding@home project results are right? Since we are quite sure F@H is working right and following this ...
5
votes
1answer
474 views

what is the meaning of VIP-cre::LSL-TOM::SOMGFP(GIN) mice?

I am reading the following paper and the researchers use "VIP-cre::LSL-TOM::SOM-GFP(GIN)" mice (page 4) for multi cell patch clamp and "VIP-cre::LSL-TOM and SOM-cre::LSL-TOM" mice injected ...
0
votes
1answer
52 views

Is it feasible to grow edible crops for lawns?

Is it possible to grow some type of plant that functions well as a lawn (e.g. is somewhat grass like), but that is humanly edible? Would anything that currently exists fit this role? For example ...
0
votes
1answer
43 views

Computational engineering of protein binding pocket

I have an X-ray structure of an enzyme with reported activity to a small molecule. This activity is rather low since it is not the native substrate. I can run molecular modeling simulations (e.g. ...
5
votes
2answers
133 views

What is protein folding and how is it relevant to disease?

I am trying to understand what is protein folding and how it could help cure some diseases. When reading articles about it, it looks like the goal is to find perfect folds for proteins because some ...
3
votes
2answers
171 views

Sheep vs. Furry animals

Why can't we take the fur of a fox without killing or skinning it just like the sheep , what's the biological or chemical structure or even the trait which is on a sheep's body that separates wool ...
8
votes
3answers
427 views

How do we know if the folding@home project results are right?

Some of us are involved in the folding@home project, spending time, money, and resources. I would like to know the answer to two main questions: How do we know we fold it right? I mean, these models ...
-1
votes
1answer
500 views

How far can we go in engineering our own evolution? [closed]

After recently seeing Christopher Nolan's Interstellar and reading Kip Thorne's The Science of Interstellar, I've seriously been reflecting on the challenges mankind faces in becoming space pioneers, ...
1
vote
1answer
30 views

Could the proteome of E. coli be fluorescently labelled?

What proportion of the total number of proteins in E. coli could be fluorescently labelled for PALM/STORM imaging?
3
votes
1answer
44 views

Has any research lab done serious work to engineer new bacteria which assemble graphene wafers?

I was thinking about crazy uses for engineered bacteria. Nano-assembly of Graphene seems like a potentially excellent target for the technology. Have any research/full-blown labs worked on this? Any ...
8
votes
0answers
694 views

Rosetta ab initio prediction and protein-protein interaction fitness help [closed]

I have designed several proteins which I predict have interactions with another protein using the sequence based Conjoint Triad Method. I would like to know which ones structurally are predicted to ...
24
votes
1answer
659 views

Phagemid display

If I'm using a bacteriophage for phage display and I'm trying to avoid avidity effects by using a helper phage what would be the best way to maintain a large library size while keeping everything ...
7
votes
1answer
534 views

What is the fastest way to build an alanine scanning library?

For interfacial studies, I would like to build an alanine scanning library for one of my proteins examining 20 sites. I will ultimately express the gene using E.coli cell-free protein synthesis. I ...