Questions tagged [protein-interaction]
The protein-interaction tag has no usage guidance.
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Alternatives to His-tags
What are the alternatives to using a histidine-tag in detecting protein-protein interactions? Why would they be more beneficial than his-tags?
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What are the structural characteristics of a protein-protein interaction network
I am an researcher in computer science. I am interesting in the alignment problem of PPI networks of difference species. I know a PPI network is a graph in its nature. I want to know whether there are ...
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C-myc/anti-myc Ab Interaction with Fusion Proteins
I am going to prepare a c-myc fusion protein with the following configuration:
(28-residue signal sequence)-(c-myc)-(GGSGGGSG Linker)-(Protein of Interest (POI))
POI is a transmembrane protein and ...
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How to find a Cancer PPI network data [closed]
I need a Protein Protein Interactions network of Cancer (any type - just I want a PPI of any type cancer), I search and see DIP PPI and ....
How to specify that is Cancer PPI and how to get a PPI of ...
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What is the significance in an alpha-helix being right-handed or left-handed?
Why is that often when alpha-helices are discussed, it is also mentioned their direction - right-handed (clockwise) or left-handed (anti-clockwise)?
I have heard that left-handed alpha-helices are ...
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Are multi-chain proteins synthesized as one biological unit?
Are multi-chain proteins (especially homo/hetero-dimers) synthesized together as one overall unit or are they separate monomers which bind together at some point after synthesis, and are there any ...
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Why are VAL, MET and ALA substitutions commonly used for protein behaviour and function studies?
I have seen that amino acids are commonly replaced with VAL, MET or ALA to study the effects of these specific substitutions. Why are these specific amino acids used in particular, what are the ...
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How is Glycophorin A and straphylococcal related to Escherichia coli and what does readily purified mean in this context?
I am reviewing the paper "Glycophorin A Dimerization Is Driven by Specific Interactions between Transmembrane Alpha-Helices." There is a statement in the abstract which I don't understand:
"The ...
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Interaction study of oligomeric proteins
I'm dealing with two different protein(say, protein_a and protein_b) which stays in an interacting-oligomeric form in biological system.
I have so far successfully been able to purify both the ...
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What tools can help me determine if a plant homologous protein interacts with an ncRNA in the same way?
First post here, so bare with me if I violate any etiquette or formatting rules.
Let's say I've got a protein in humans. When it interacts with an ncRNA found in humans, it does something that I'm ...
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Determining the appropriate bioconjugate
I'm thinking of crosslinking two proteins. The crystallographic nature of the interaction and the binding motif for each molecule is know. The paper says the intermolecular contacts (over 50; van der ...
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What is the difference between options protein and replication in the NCBI database?
After checking the NCBI help page, I am still unclear about the difference between protein and replication interactions for HIV.
http://www.ncbi.nlm.nih.gov/genome/viruses/retroviruses/hiv-1/...
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In protein-protein interactions what is the difference between a binding site and an interface?
I see binding site and interface used almost interchangeably in the literature, but I'm not sure if it is exactly the same thing or not - what is the difference?
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Help with STRING database data
I'm working with data downloaded from STRING database (string-db.org) for protein-protein interactions.
My idea is to compare the topology of connections of the same protein on different organisms.
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What is the protein with the highest affinity to lactose?
I was wondering: What is the protein with the highest affinity to lactose? Are there some databases with values for affinity?
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protein-binding sRNAs
I am actually a computational biologist, pardon me if my question description is a bit off-note, my curiosity is driving me on
A bit of background and context:
In bacteria, a regulatory protein ...
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I2D vs STRING for protein-protein interactions
Can someone clearly explain me the differences between STRING and I2D(Interlogous interaction database) ? What does each database do best ? Which database is better for what kinds of studies etc ?
user6417
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Why are low throughput methods more trustable for protein interaction?
I read that protein interactions found using high throughput methods are less trustable than those found using low throughput methods.
For example, searching for interaction of BRCA2 on BioGRID, I ...
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What are the differences between HPRD and BIOGRID databases?
What are the differences between HPRD and BIOGRID protein-protein interactions databases?
What are their purposes? Why do we need two different databases?
How is data collected into each one?
How ...
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