Questions tagged [recombination]
The process of breaking and recombining strands of DNA.
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How to calculatie frequency of recombination between two genes based on their coordinates in ENSEMBL?
I have two genes:
https://www.ncbi.nlm.nih.gov/gene/326619 and https://www.ncbi.nlm.nih.gov/gene/326620
both are mapped to chromosome 13. First is at position 23944778..23945232 second is at positions ...
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Recombinations between Distant coronaviruses
With recombination I mean long ones, say > 1000 nucleotides, so that the two parents must be coronaviruses and the two pieces of genomes must have compatible genomic coordinates for the recombinant ...
2
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1answer
123 views
Number of homozygous recessive offspring
A question from the KVPY-SX examination, organised by the Indian Institute of Science (IISc) in Bengaluru, India, held on 3rd November, 2019.
A plant heterozygous for height and flower colour (TtRr) ...
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How can we know which alleles are together on a chromosome?
This illustration says that if the two homozygotes pr+ pr+ vg+ vg+ and pr pr vg vg are crossed to produce a heterozygous offspring (pr+ pr vg+ vg), then:
this cross gives us exactly what we need to ...
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1answer
118 views
What is the map unit between the genes?
While doing some biology I came across this question:
The following tables present results of plant crosses involving two linked genes: S is a seed-color gene, and L is a plant-height gene. Each gene ...
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1answer
65 views
What is the probability of having a son with blood type O? [closed]
So I was solving some biology questions, and this question left me confused. The response is supposed to be 22% according to the answers, but all the methods that I used did not lead me to it. I ...
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1answer
125 views
How can I change the payload of a bacteriophage used to transform E. coli?
I was looking at bacteriophages and how they're used to transform E.coli. While the whole process of how a bacteriophage works makes sense theoretically, I wanted to know how one goes about changing ...
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What is the correct nomenclature for expressing a genotype where a recombination event may occur?
Given an example punnett square:
...
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1answer
115 views
Confusion about how to compute probabilities for linked genes
I am confused about how to compute probabilities for linked genes.
The problem that I am solving is this:
P generation: AAbbddEEff X aaBBDDeeFF. If A and B are 40 centimorgan apart, what is the ...
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2answers
73 views
Using punnett squares to express sex/gene linkage and recombination frequency
I have been using punnett squares to visualize mating experiments. I am looking at a pairing which is known to have a crossover event occur. I know from empirically collected data the recombination ...
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1answer
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Why V(D)J recombination only happens in B and T cell development?
Does V(D)J recombination only happen in B and T cell development? Can it happen in other types of cells?
If V(D)J recombination only happens in B and T cell development, why other types of cells ...
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1answer
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Clarifying distinction between genetic recombination , translocation. and transposition
Having thought about the distinciton between these terms I have come up with the following definitions, are these correct?
Translocation describes the relocation of a chromosomal segment to a ...
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QA/monitoring in recombinant vaccine manufacturing
On a16z[0] podcast on vaccines[1], an interesting tidbit came up: vaccine manufacturing in cell cultures is expected to hit a major challenge in terms of quality control due to unpredictable behaviour ...
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How many of the four meiotic daughter chromosomes of a homologous pair can be recombinant via crossover?
In graphics I've seen, crossing over occurs between the "inner" two chromatids in a side-by-side arrangement of two duplicated chromosomes:
This suggests that only two of the four meiotic daughter ...
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1answer
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How can prokaryotes repair double strand breaks by homologous recombination if they're haploids?
Title says it all, I must be going crazy.
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Why is Homologous Recombination (HR) more frequent at long sequence repeats?
I'm studying plasmids in bacteria (E. coli), and trying to understand the well-cited phenomenon that recombination frequency increases with longer repetitive sequences. I think this also applies to ...
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Meiotic recombination hotspots
Im trying to find a proper and general file with chromosomal coordinates for meiotic recombination hotspots.
I know that ucsc hgtables, has a table with recombination regions and their recombination ...
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Are highly-polyploid organisms more resistant to ionising-radiation-induced DNA damage?
Homologous recombination, although most famous for its use to mix together maternal and paternal alleles during meiosis, is most commonly used as a DNA-repair mechanism, allowing cells to repair ...
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1answer
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Help with gene linkage and mapping!
Here's an interesting question I've been trying to figure out:
My annotations are in red. So far, I've figured out:
We know 630 colonies survived when plated with ACD antibiotics...meaning they ...
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0answers
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chromosome 19 and recombination
Im doing a project with structural variation created by recombination within the human genome during spermatogenesis, where im especially examining intrachromatid homolog recombination.
I find that ...
2
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1answer
407 views
Insertional inactivation using pBR322
I have questions regarding "selection using insertional inactivation".
In a typical DNA recombination experiment using plasmid pBR322 where a new gene in inserted in the place of tet gene, it is ...
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70 views
Why do genetic testing companies (FTDNA,AncestryDNA,23andme) express DNA shared in centimorgans (cM) instead of in number of base pairs or in percent?
Expressing DNA shared in number of base pairs or in percent of total genome would be so much simpler and easier to understand for the customers.
.
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How did the molecular machinery for recombination originate?
I'm wondering about the origins of genetic recombination. During crossover new chromosomes are created. They have different allele combinations from the original two chromosomes. This process allows ...
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How is the right amount of nucleotides synthesised during homologous recombination?
I'm reading about homologous recombination in the context of double-strand break repair. It seems like the trick is that around the break, homologous recombination uses a template duplex to generate ...
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334 views
Cis and trans linkage
Question:
Two dominant non-allelic genes are 50map units apart, the linkage is:
(A) Cis type
(B) Trans Type
(C) Complete
(D) Absent/Incomplete
My take:
After a lot of searching on the net I found ...
3
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1answer
115 views
Difference between crossover rate and recombination rate?
I am not a biology student and therefore, need clarification if crossover rate and recombination rate are the same thing. So if the text says 'recombination rate per base pair per generation' or '...
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2answers
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How is a specific fragment isolated for PCR amplification?
For background I am interested in studying engineering applications of a specific protein, which is not commercially available. My end goal is to express the gene for the protein in bacterial cells, ...
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Join a linear plasmid just using a primer (without ligase enzyme)
Can I use just a primer and PCR to join the cutted plasmid like this ? without using any further enzyme like ligase.
After the linear dsDNA plasmid annealed into 2 ssDNA, primer will bind to middle. ...
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1answer
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Lambda red recombination without electroporation
All protocols that I found about Lambda red recombination are using Electroporation as a method to introduce (inject) the homologous DNA (usually a PCR products or a linear dsDNA) to the E.coli cell. ...
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1answer
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Are there chromosomes that we inherit that are not recombinated? [closed]
We do have chromosomes that recombinated ,but aren't there pure chromosomes that are not recombinated that people would have?
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1answer
548 views
How would one go about isolating a specific mRNA out of a pool of others? [closed]
I'm writing an essay about DNA recombinant techniques where the question asks to outline all the steps to arrive at having a colony of bacteria expressing a gene of interest.The question makes you ...
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DNA strand displacement and Branch migration- the same or different or interrelated?
So, recently I have begun studying DNA strand displacement and then I come across the term 'branch migration' (p.s. I am not a biology major student). What I understand is that branch migration is ...
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1answer
141 views
How would homologous recombination and mutations affect speciation?
I'm wondering how homologous recombination and mutations can affect how speciation can occur from one species (so that 2 will be created).
I'm doing research and I found that different mutations and ...
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2answers
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How do biologists determine the parents of a child
I am not well-versed in biology so this question might be wrong. As far as I understand meiosis, two germ line cells with 23 chromosomes each (one cell from the father and another from the mother) ...
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1answer
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Why does the insulin taken from pig when injected into humans causes allergy but not the insulin taken from bacteria?
At earlier times, insulin was injected to human patients that were taken from pigs and horses. But that caused allergy it seems.
But why is the insulin produced by the recombinant bacteria not ...
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1answer
226 views
How exactly are genes swapped during the process of recombination?
I am studying recombination in Meiosis and the idea of crossing over doesn't make sense. My understanding is that 46 chromosomes in our germ-cell (23 from mom + 23 from dad) line up next to each other ...
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Question about genetic recombination
I am having some difficulty understanding a few things about genetic recombination, in part because of confusion from different diagrams in books.
First of all, I wanted to verify whether I have ...
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Need clarification on Coalescent with Recombination
I've been reading about recombination with coalescent. The link to that file is as follows:
http://www.stats.ox.ac.uk/__data/assets/pdf_file/0008/4688/Reading_for_Recombination.pdf
I get the part ...
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1answer
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Questions in Regards to Isolating Promotor Region for Recombinant DNA Techniques
For a while now I have been trying to isolate the promotor region of this Protein.
Using sticky end PCR, I need to isolate this promotor region so it has paticular sticky ends for the purpose of ...
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How is randomness of the branch migration process ensured?
Branch migration (part of genetic recombination) is a random process. It looks like Ruv-ABC proteins are mainly responsible for this process, but how is randomness ensured? After all, it can only be a ...
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1answer
134 views
Restriction enzyme digestion
A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B. This produces a 3000 bp and a 2000 bp bands when visualized on an agarose gel. When digested with one ...
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Heteroduplex and Products of cleavage of Holliday Junction (Resolution) [duplicate]
In homologous recombination (Holliday model), the last process i.e resolution can occur in two ways (horizontl and vertical cuts) leading to formation of either "splice" or crossover products or "...
0
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2answers
253 views
Why does meiotic recombination disrupt the independent assortment of genes on the same chromosome?
There are many sources that say that genes that are "linked" by being on the same chromosome assort with each other -- that when an allele is passed on that the gamete containing said allele will also ...
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1answer
165 views
Data on Recombination rate and Gene Positions in Humans
I am looking for estimates of per base pair recombination rate in humans and indication on gene positions. The goal is to be a able to plot recombination rate in the y-axis and position (in bp) along ...
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1answer
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What is the difference between conditional and inducible activation?
In the modern genetically modified animal models, there are not only constitutive mutations which start producing proteins already in the embryo, but also mutations which can be turned on later. I've ...
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1answer
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meaning of "usually greater than 200 base pairs"
From "Risks from GMOs due to Horizontal Gene Transfer", by Paul Keese:
Homologous recombination. All cellular organisms have molecular functions dedicated to recognizing and recombining DNA ...
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1answer
452 views
Recombination fraction( frequency) in Homozygotes
I have a question about the recombination frequency I hope you have time to answer.
Background: If we look at homozygous individual A/A B/B which has been formed from the gametes AB and AB, this ...
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2answers
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Genetic linkage greater than 50 centimorgans
Classically, the linkage between two loci can be measured in centimorgans (cM), which represents the percent chance that these two loci will recombine an odd number of times (generating a recombinant ...
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1answer
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How do I find the sequence of the phage BxB1 gene?
Specifically, I'm trying to find the sequence of BxB1 and its att sites. I have success finding the att site sequences, but I'm having a bit more of an issue with finding the sequence of the actual ...
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1answer
952 views
Which process is right to describe V(D)J recombination? RAG-1 and RSS recurring process
I'm studying V(D)J recombination. I think I have two incompatible books about explantaion of the process. Which is right?
In Molecular Biology of the Cell 5th Ed., firstly RAG (-1?) combines to RSSs ...
