Questions tagged [recombination]

The process of breaking and recombining strands of DNA.

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How is a specific fragment isolated for PCR amplification?

For background I am interested in studying engineering applications of a specific protein, which is not commercially available. My end goal is to express the gene for the protein in bacterial cells, ...
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Are there chromosomes that we inherit that are not recombinated? [closed]

We do have chromosomes that recombinated ,but aren't there pure chromosomes that are not recombinated that people would have?
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Possible Genotypes — Linked Genes

Please consider the following question: Genes A and B are located on the same chromosome. If two individuals with the genotype shown below are crossed, which genotype could be formed? Let / ...
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What is a “tool strain”?

When a biologist is talking about a genetically engineered mouse strain which is a "tool strain", what does that mean? What is the exact definition of a tool strain? What is the difference between a ...
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What is the map unit between the genes?

While doing some biology I came across this question: The following tables present results of plant crosses involving two linked genes: S is a seed-color gene, and L is a plant-height gene. Each gene ...
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Confusion about how to compute probabilities for linked genes

I am confused about how to compute probabilities for linked genes. The problem that I am solving is this: P generation: AAbbddEEff X aaBBDDeeFF. If A and B are 40 centimorgan apart, what is the ...
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Questions in Regards to Isolating Promotor Region for Recombinant DNA Techniques

For a while now I have been trying to isolate the promotor region of this Protein. Using sticky end PCR, I need to isolate this promotor region so it has paticular sticky ends for the purpose of ...
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Restriction enzyme digestion

A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B. This produces a 3000 bp and a 2000 bp bands when visualized on an agarose gel. When digested with one ...
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Why does meiotic recombination disrupt the independent assortment of genes on the same chromosome?

There are many sources that say that genes that are "linked" by being on the same chromosome assort with each other -- that when an allele is passed on that the gamete containing said allele will also ...
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How can we know which alleles are together on a chromosome?

This illustration says that if the two homozygotes pr+ pr+ vg+ vg+ and pr pr vg vg are crossed to produce a heterozygous offspring (pr+ pr vg+ vg), then: this cross gives us exactly what we need to ...
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What is the probability of having a son with blood type O? [closed]

So I was solving some biology questions, and this question left me confused. The response is supposed to be 22% according to the answers, but all the methods that I used did not lead me to it. I ...
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QA/monitoring in recombinant vaccine manufacturing

On a16z[0] podcast on vaccines[1], an interesting tidbit came up: vaccine manufacturing in cell cultures is expected to hit a major challenge in terms of quality control due to unpredictable behaviour ...
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Why is Homologous Recombination (HR) more frequent at long sequence repeats?

I'm studying plasmids in bacteria (E. coli), and trying to understand the well-cited phenomenon that recombination frequency increases with longer repetitive sequences. I think this also applies to ...
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Why do genetic testing companies (FTDNA,AncestryDNA,23andme) express DNA shared in centimorgans (cM) instead of in number of base pairs or in percent?

Expressing DNA shared in number of base pairs or in percent of total genome would be so much simpler and easier to understand for the customers. .
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How did the molecular machinery for recombination originate?

I'm wondering about the origins of genetic recombination. During crossover new chromosomes are created. They have different allele combinations from the original two chromosomes. This process allows ...
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Cis and trans linkage

Question: Two dominant non-allelic genes are 50map units apart, the linkage is: (A) Cis type (B) Trans Type (C) Complete (D) Absent/Incomplete My take: After a lot of searching on the net I found ...
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Join a linear plasmid just using a primer (without ligase enzyme)

Can I use just a primer and PCR to join the cutted plasmid like this ? without using any further enzyme like ligase. After the linear dsDNA plasmid annealed into 2 ssDNA, primer will bind to middle. ...
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Lambda red recombination without electroporation

All protocols that I found about Lambda red recombination are using Electroporation as a method to introduce (inject) the homologous DNA (usually a PCR products or a linear dsDNA) to the E.coli cell. ...
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DNA strand displacement and Branch migration- the same or different or interrelated?

So, recently I have begun studying DNA strand displacement and then I come across the term 'branch migration' (p.s. I am not a biology major student). What I understand is that branch migration is ...
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Heteroduplex and Products of cleavage of Holliday Junction (Resolution) [duplicate]

In homologous recombination (Holliday model), the last process i.e resolution can occur in two ways (horizontl and vertical cuts) leading to formation of either "splice" or crossover products or "...
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Why V(D)J recombination only happens in B and T cell development?

Does V(D)J recombination only happen in B and T cell development? Can it happen in other types of cells? If V(D)J recombination only happens in B and T cell development, why other types of cells ...

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