Questions tagged [ribosome]

Organelles responsible for protein synthesis in cells. They are distinguished into "free" and "membrane-bound" classes.

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11
votes
3answers
27k views

Why do 60S & 40S ribosomal subunits make an 80S ribosome (not 100S)?

Why do 60S & 40S subunits make an 80S (not 100S) ribosome and, similarly, 50S & 30S make 70S? 60+40 is not equal to 80, nor is 50+30 equal to 70, so why are the subunits of the 80S and 70S ...
6
votes
2answers
667 views

Can ribosomes read ssDNA?

My question is whether translation can be done, either naturally or artificially, through a ribosome reading (single-stranded) DNA directly. If not, I would like to know what allows ssRNA to be ...
24
votes
1answer
7k views

What is the criticality of the ribosome binding site relative to the start codon in prokaryotic translation?

In prokaryotic translation, how critical for efficient translation is the location of the ribosome binding site, relative to the start codon? Ideally, it is supposed to be -7b away from the start. ...
15
votes
1answer
803 views

Can DNA act as a translation substrate?

I get conflicting answers. One would think if it was true, it would be rather seminal and widely known. There are papers from Khorana[1], Holland[2], and Bretscher[3] (late 60s) that suggest that it ...
7
votes
4answers
16k views

RNA or ribosome, which one moves during translation?

During translation ribosomes decode the genetic information present in the mRNA and protein synthesis takes place. During this process which of those two does move, the ribosome or the mRNA?
5
votes
1answer
225 views

What is the distance between the 3' 18s rRNA (the Kozak consensus sequence) and the A-site of eukaryotic ribosomes during protein translation?

Reposted from Quora The Kozak sequence is shown to be important in translation initiation, and the A-site is the anti-codon recognition site. If the distance is not known, how could I find out what ...
2
votes
1answer
620 views

Translation of Poly-U in the Nirenberg and Matthaei experiment

In the Nirenberg and Matthaei experiment the artificial mRNA, polyU, was translated into polyphenylalanine in a cell-free system, establishing that UUU was the codon for Phe. How did this work as the ...
3
votes
2answers
1k views

Why does azithromycin not affect human mitochondria?

Drugs like tetracyclines, macrolides and aminoglycosides bind to prokaryotic ribosomes. It is interesting that our body too having mitochondria, which have prokaryotic ribosomes, there is little(?) ...