Questions tagged [rrna]

Ribosomal RNA - builds together with the protein subunits the Ribosome. It is the most predominant material of the ribosome, approximately 60% of the weight are made of rRNA and 40% of proteins.

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Where can I obtain the sequence of the E. coli ribosomal RNA precursor?

I have found separate sequences for 16S rRNA (from the small subunit) and 23S rRNA and 5S rRNA (from the large subunit). I need the full precursor rRNA sequence, which I cannot find. However the ...
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How to do desalt reaction by passage through a Micro Bio-spin 6 column?

I want to perform the bisulfite sequencing on rRNA to detect the 5-mC. I followed this protocol • DNA-free™ DNA Removal Kit (Invitrogen) • Purified RNA, Treat RNA according to manufacturer's ...
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How to map the N6-dimethyladenosine by primer extension?

I am trying to map N6-dimethyladenosine on rRNA using primer extension (low dNTPs assay) method. But i am not able to detect map my position. I went through some articles they mentioned like they ...
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16S rRNA and 5S rRNA datasets

I need 16S rRNA and 5S rRNA (Gammaproteobacteria - E.coli) Secondary structure files in ct format. I tried looking for them in different RNA databases, could not find one. Can someone tell me where ...
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Ribosomes producing proteins, but need proteins to be produced?

So according to my textbook: RNA is used to create ribosomal RNA (known as rRNA) which is then combined with proteins to form the ribomsomes necessary for protein synthesis. I'm a bit confused ...
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16s rRNA Sequencing From Gut Microbiome (stool)

Do I extract RNA or DNA from gut microbiome (stool samples) if want to do 16s rRNA sequencing?
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550 views

How is 16s rRNA used to identify unknown bacteria?

How is 16s rRNA used to identify unknown bacteria? With PCR the 16S rRNA is amplified and sequenced, but how can you identify the bacteria with this 'piece of knowledge' if the 16S rRNA is conserved ...
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Are upstream activating factor (UAF) and upstream binding factor (UBF) the same thing?

During ribosome pre-40S and pre-60S synthesis, many sources state the importance of UAF or UBF in initiation complex of ribosome DNA transcription. None of the sources I've seen mentions the other ...
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Why is 5S-rRNA is different from other rRNAs in place of transcription and usage of RNA polymerase?

While transcription of rRNAs happens in nucleolus mediated by RNA Polymerase-I, we see that 5S-rRNA is transcribed elsewhere by RNA Polymerase-III. What is the cause and why?
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178 views

What do cells do when they aren't creating proteins?

I've always thought that the majority of the "work" in a cell is protein production, until I read the following. The Wikipedia article on the central dogma of molecular biology states this: 80% of ...
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How to build S16 rRNAs phylogenetic trees?

Here is what I know so far: Multiple copies are present in prokaryotes, but according to a paper by Lukjancenko el al.[1] it is enough to select the most similar ones. There are stem regions in the ...