Questions tagged [sds-page]

Questions pertaining to sodium dodecyl sulfate polyacrylamide gel electrophoresis, a technique used to separate proteins according to electrophoretic mobility

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How do SDS-PAGE gels differ in a Bis-Tris system vs. a Tris-Glycine system?

Protein migrate differently in Bis-Tris and Tris-Glycine gels. I was curious about the actual reasons why. Do certain gel systems result in a tighter resolution than others?