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Questions tagged [small-rnaseq]

Next-Gen Sequencing of RNA sample enriched with small RNAs (~15-200nt) such as miRNA, piRNA etc.

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Precursor miRNA and a mature miRNA

What is the main difference between a precursor miRNA and a mature miRNA? It is often the case that we have more than one precursor miRNA but only one mature miRNA. The miRNA-seq data contains only ...
sammy's user avatar
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2 votes
1 answer
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Problems with analysis of small RNAseq data - Adapter trimming

I have always faced a problem while analyzing small RNAseq data, at the step of adapter trimming. Overview of small RNAseq (Illumina) RNA is size fractionated using columns or PAGE 3' and 5' adapter ...
WYSIWYG's user avatar
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small RNA bioanalyzer traces showing miRNA enrichment for sRNA-seq

I am bioinformatician trying to support some small RNA-seq experiments. All bioanalyzer traces that I have seen for RNA at the small RNA range show at peak at 22. But probably it depends on the ...
lpantano's user avatar