Questions tagged [splicing]
Splicing is a modification of an RNA after transcription, in which intervening nucleotide sequence sequences (introns) are removed and the remaining sequences (exons) are joined.
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Is exon order always preserved in splicing?
Are there any cases in which the splicing machinery constructs an mRNA in which the exons are not in the 5' -> 3' genomic order? I'm interested any such cases, whether they involve constitutive or ...
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What are some examples of genes that code for multiple proteins?
The title pretty much says it all. It is widely taught that a gene in a eukaryotic system could produce more than one protein due to post-transcriptional modification, but I do not believe I have come ...
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What percentage of introns are spliced in polyadenylated mRNAs?
I am asking a variation of a previously asked question: What percentage of RNA splicing happens during transcription?
I am planning mRNA purification using poly(A) antisense-oligo pull-down and, for ...