Questions tagged [staining]

Set of methods to visualize certain properties (structure, gene expression, potential changes, etc.) of organisms or single cells.

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How a pathologist would analyse this H&E image?

I am working on a project which involves writing computer software to analyse histological images. A typical image looks like this: It is a Hematoxylin and Eosin stained biopsy of breast cancer ...
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How does gold chloride stain neuromuscular junctions?

Gold chloride is used in muscle biopsies to stain the NMJ. What is the substrate that it binds to?
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Hoechst red and blue spectra - what does the red emission tell us?

I understand that the blue spectrum is emitted when Hoechst 33342 binds to nuclear DNA. But what exactly is the red spectrum telling us? I read that the red spectrum can be indicative of apoptosis, ...
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287 views

Common stains for fungal microscopy

I recently got a light microscope to start exploring microscopic features of fungi (and to explore microscopy in general). What are some of the most common and easily available stains used when ...
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DRAQ5 and PI incompatible?

I'm interested to analyse hemolymph cells from a marine mollusc. I wanna count only "alive" cells so my strategy was the following: DRAQ5+ (nucleated cells) and PI- (alive cells) and identify ...
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Is there any double staining method to visualise separate colour for plant tissue and fungal tissue?

Is there any double staining method for developing separate colour on fungal tissue and plant tissue when the said 2 types of tissues are intermingled? Unlike my previous question, this question not ...
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82 views

Dark and bright sides in fluorescence microscopy images?

I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that one part of image ...
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34 views

Is it possible to color sperm cells so they look more clear under a microscope?

Im working on something that requires me to check the velocity of a sperm cell under a microscope but I imagine it would be very hard for a program to keep track of the sperm cells.
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294 views

DAPI staining showing unknown artefacts?

Dear fellow researchers, I have been facing some problem with staining nucleus with DAPI. I use the standard protocol for fixation 4%PFA+0.1% TX-100+2%BSA. I use 0.2ug/ml conc. of DAPI for 5 minutes....
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How can I stain only the Chitin but not Cellulose or Glucans?

Is there any stain, that binds specifically to chitin but not to cellulose? (for differential staining and double staining purpose) It seems the lactophenol-cotton blue, aniline blue, trypan blue etc ...
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Re-stain slice using a different secondary antibody?

I stained a free-floating ~300uM brian slice using 4 different 1º antibodies (rabbit, chick, mouse, rat). For the mouse primary, I mistakenly used an Alexa-350 secondary, when I should have used 594. ...
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83 views

How to use DRAQ5 and MitoTracker CMXros dyes to stain turtle erythrocytes?

I am working on turtle species and want to use DRAQ5 and MitoTracker CMXRos to stain turtle nucleated erythrocytes. Can anyone suggest me a protocol to carry this stain out? Would the two stains ...
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Barr body Giemsa staining

Not all the female cheek cells show Barr Bodies when stained with Giemsa stain? Only 30 to 40% female cells show Barr Bodies. Why? Our Experiment: We washed mouth twice with listerine to reduce ...