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Questions tagged [sterilisation]

Sterilization: any process that eliminates or kills all forms of life and other biological agents present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media. Sterilization can be achieved by heat, chemicals, irradiation, high pressure, and filtration.

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How do I determine an acceptable $F_0$ value in sterilization?

Here's a sample question: The sterilization process using moist heat is used to kill B.stearothermophilus by heating to 115 degrees C and holding it for 30 mins. For B.stearthermophilus, it ...
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Effects of uv-c 280nm 1.200uW-sec LED radiation on water?

I recently ordered a LARQ bottle. It's a drinking bottle for water that is equipped with a UV-C LED 280nm in the cap. It switched on every 2 hours and thus purifies the water. It provides a 1.200uW-...
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Cleaning out Agar from Autoclave Reservoir

I have a Tuttnauer EZ10 autoclave where the previous user poured m-Endo Agar into the reservoir. Using vinegar, we've been able to remove most of it, but there is a thin film of purple residue coating ...
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Micro-propagation of Moss in Agar (sterilization?)

I'm having trouble sterilizing my moss sample for micro-propagation. Moss is basically a sponge and it has a massive surface area. I tried Isopropyl alcohol figuring it had a very high probability of ...
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what is the reason for using 70% alcohol (ethanol)l? [duplicate]

For the purpose of sterilization I am using 70% alcohol (ethanol) for a really very long time. but whenever I ask about why actually we are using 70% alcohol till now I did not get a very specific ...
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Sterilize/disinfect sugar for lab use

How do I sterilize/disinfect ordinary table sugar for lab use? I'm using the sugar in an agar and I want it to be as clean as possible. Are there any effective, conventional ways of doing this?
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When is strict sterile technique necessary? Cloning vs. protein expression

Background: I have previously worked with RNA, and then we use laminar flow hoods for any work where we do plating or inoculation of cultures while applying sterile technique. I am now working in a ...
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Using UV to kill bacteria in shoes

I see that UV-C is the best to kill bacteria generally. We are interested in using to kill off odor in shoes. What illuminance is needed to kill most bacteria with UV-C? Thanks!
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Why is 70% ethanol preferred for aseptic techniques?

Are other concentrations (say 80%) less effective,or is this just for convenient manufacturing? Is the concentration chosen only because it is less volatile than 100 percent ethanol and hence safer?
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What is effectiveness of methyl-alcohol as sporicide?

Methyl-alcohol is the weakest alcohol in terms of bactericidal properties. However, I have read that it also works as sporicide. What is the effectiveness of methyl-alcohol in killing the spores of ...
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How sterile is sterile when working with nucleic acids to prevent contamination?

I am reading up on preparatory work on working with nucleic acids and a lot of the instructions speak on excessive procedures on cleaning environments with high %ethanol and making sure the equipment ...
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Are there sterilisation methods that do not denature proteins as heat does?

Context: Most countries require milk to be sterilised through radiation or heat to remove possible harmful bacteria. Both of these processes denature the proteins in the milk (ref). Are there methods ...
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Hydrogen Peroxide on Open Wounds

I know that H2O2 is a chemical with high oxidative potential. It can deliver nascent oxygen which can destroy bacteria. I understand that open wounds have cells that are dividing at a fast rate to ...
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Will an 11W UVC lamp kill bacteria?

If I shone a UVC lamp (11W) on something (say for example a plastic phone case) for an hour, would that kill off a noticeable amount of bacteria? That is, if I were to go over the case with a cotton ...