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Questions tagged [t7-promoter]

Promoter from the bateriophage T7. The promoter is highly specific for the T7-RNA polymerase and widely used in the field of molecular biology as promoter for controlled gene expression.

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How do I reverse my insert within a plasmid?

I have a plasmid with: ...T7pro. -- RBS -- XBa1 cut site -- ProDpro. -- RBS -- AmilCP -- T -- Spe1 cut site -- T... How do I design primers to reverse the portion in bold, such that the T7 and ProD ...
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What is the space between -10 promoter sites and start sites (+1) [closed]

Between the transcriptional start site of a gene and the -10 promoter region there is always a space of around 10 nucleotides, what is this space function and purpose? Do you see the space between ...
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683 views

Gene configuration : Divergent vs convergent

I start reading a paper where they discuss the effect of the divergent promoter/gene and the convergent promoter/gene on the genetic expression of an organism. So I want to know what is the ...
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Are the human genes' promoters all known?

It seems to be a basic question, but I couldn't find a certain answer. The human genome is known for more than a decade, and is available through several data providers as the NCBI etc. For the genes'...
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T7 vs pBAD promoter strength

How do pBAD and T7 promoter strengths compare when pBAD is induced under conditions which would lead to its highest strength? Are there any papers that compare the two inducible promoter systems?
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What is the highest number of genes expressed under one T7 promoter?

What is the highest number of genes expressed under one T7 promoter? Also, what is the longest polycistronic mRNA? I am interested in the context of heterologously expressing biosynthetic gene ...
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Why does pETDuet-1 only have one T7 terminator but two T7 promoters?

Why does pETDuet-1 only have one T7 terminator but two T7 promoters (vector map below)? Is there any advantage of having only one terminator? There are going to be two mRNAs transcribed from this ...
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633 views

T7 phage promoter action in mammals

Does anyone know if the T7 phage promoter is efficient in mammalian systems?
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Why does the T7 RNA Polymerase require a reducing environment ie. DTT

Every bloody protocol suggests adding in DTT when doing in vitro RNA transcription. Why? The rationale seems to be that the cytoplasm traditionally has a reducing environment but as the only protein ...
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What determines a successful protein expression in E. coli?

Some proteins express well in a heterologous host; others- don't. A few requirements are known to determine the protein expression, like a strong promoter (like T7) for transcription and a strong ...