Questions tagged [transcription]

The process that produces a complementary strand of RNA from a section of DNA or (rarely) other RNA.

29 questions with no upvoted or accepted answers
Filter by
Sorted by
Tagged with
3 votes
0 answers
47 views

What is the "anatomy" of transcriptional regulation in Archaea?

I want to know what are the DNA elements that are recognized by transcription factors in Archaea and so what is the "anatomy" of transcriptional regulation in this case. I found in this ...
Manuela's user avatar
  • 443
2 votes
0 answers
25 views

Strong activation of MAPK signaling pathway

I am reading a paper named "Mathematical models of protein kinase signal transduction". In that paper, I encountered a condition called "strongly activated pathways". In that ...
이영규's user avatar
2 votes
0 answers
326 views

Sequence between PolyA signal and PolyA tail

Between the polyadenylation (PolyA) signal (AAUAAA in humans) and the start of the polyA tail, there is a short sequence of about 10~20 (G/U rich) bases, is there a special term for it? Image credit: ...
zehata's user avatar
  • 121
2 votes
0 answers
1k views

Multiple transcripts encoding for one protein

Trying to get a better understanding of the process of DNA to proteins. So when we have a gene, it is read from the 5' to 3' end, only translating the exons to mRNAs. A single gene can have multiple ...
Danielson's user avatar
  • 121
2 votes
0 answers
69 views

Link between macro lncRNA and DNA looping

I was wondering if anybody knows some publication about macro lncRNA (very long unspliced RNAs) or more generally a transcribed RNA that may lead to cis-DNA looping of genomic regions overlapped by ...
Nicolas Rosewick's user avatar
1 vote
0 answers
28 views

How I can find the list of transcription factor proteins involved in transcription of a specific gene?

I want to get the list of transcription factor proteins involved in the transcription of the human SIRT1 gene. How can I access that?
Mehad's user avatar
  • 19
1 vote
0 answers
140 views

Why isn’t prokaryotic mRNA modified post-transcriptionally like eukaryotic mRNA?

Eukaryotic mRNA is subject to addition of a methyl-guanosine cap at the 5’ end, addition of 100-200 adenosines to the 3’ end, and splicing. Why don’t these occur in prokaryotes? Is it because there is ...
HypnoticBuggyWraithVirileBevy's user avatar
1 vote
0 answers
35 views

Is there any current knowledge of how DNA polymerase is "motivated" to perform error corrections during transcription?

I was trying to understand the process of how polymerase performs error corrections on DNA. Every paper on this topic mentions what happens during the process, but there's no mention of how it happens....
Nav's user avatar
  • 527
1 vote
0 answers
42 views

Functional Difference between the Distal Promoter and Enhancer Region

According to the Wikipedia article on eukaryotic promoter regions, the distal promoter is: Anything further upstream (but not an enhancer or other regulatory region whose influence is positional/...
Hawkeye's user avatar
  • 596
1 vote
0 answers
32 views

response elements in transcription regulation

I'm studying eukaryotic gene regulation and I want to know whether there are some instances where transcription factors don't need to bind to response elements to initiate transcription. RNA ...
mj2000's user avatar
  • 56
1 vote
0 answers
101 views

Confusion about the role of capping and polyadenylation in nuclear transport

Before termination stage of transcription a GMP cap is added to the 5' end and poly-A tail to 3' end of mRNA. The link(http://sciencing.com/role-rna-polyadenylation-5434666.html) says: The poly(A) ...
Mockingbird's user avatar
1 vote
0 answers
39 views

Modifications of free nucleotides

We know that RNA can get modified post transcriptionally (RNA modification). So when the RNA is degraded, is it possible for such modifications to still be present on the individual nucleotides -- ...
Opt's user avatar
  • 131
1 vote
0 answers
623 views

Usage of the terms: Enhancer, Upstream activating sequence and Downstream activating sequence

From wikipedia An upstream activating sequence or upstream activation sequence (UAS) is a cis-acting regulatory sequence. It is distinct from the promoter and increases the expression of a ...
Tyto alba's user avatar
  • 8,782
1 vote
0 answers
92 views

Transcription by RNA polymerase in absence of inducer and activator

Does the presence of activator(CAP-cAMP complex) and inducer(lactose) only increase the likelihood that a particular gene(here operon) gets transcribed, i.e increases the rate of transcription of that ...
Tyto alba's user avatar
  • 8,782
1 vote
0 answers
58 views

Hydrogen bonding and the blocking thereof in nucleic acids during nuclear processes

In transcription, RNA polymerase unwinds the DNA double helix and begins attaching RNA nucleotides to the template strand. In its wake, the DNA double helix closes back—this is only natural, seeing as ...
lightweaver's user avatar
  • 1,933
1 vote
0 answers
89 views

Is DNA transcription inactive during mitosis ie no proteins are made? How does the cell survive?

I've read that during mitosis, DNA exists as heterochromatin, a form that is unable to be accessed for transcription. Does this mean no new proteins are made during what can be 20% of a cell's life? ...
Jon Wilcox's user avatar
1 vote
0 answers
43 views

Will bacterial promoter without either -10 or -35 sequence allow no transcription?

I have a strong constitutive promoter on a plasmid inside of E.Coli, which expresses a gene. Having either -10 or -35 sequence modified to random sequence, will it ensure zero transcription from that ...
Neil's user avatar
  • 103
1 vote
0 answers
84 views

Are upstream activating factor (UAF) and upstream binding factor (UBF) the same thing?

During ribosome pre-40S and pre-60S synthesis, many sources state the importance of UAF or UBF in initiation complex of ribosome DNA transcription. None of the sources I've seen mentions the other ...
Kenny Kim's user avatar
  • 365
1 vote
0 answers
62 views

What is the suitable terminology to describe this study approach?

I need to know the correct term(s) which are usually used in the parlance of both biology and bioinformatics for this study approach: About 11 transcripts were investigated using qPCR for a number of ...
doctorate's user avatar
  • 181
0 votes
0 answers
26 views

Speed of cell signal to protein production

How long does a cell take to make a protein from the point of receiving the signal to produce the protein? Does the cell have stores of the protein ready to release or is the protein created in real-...
webworm's user avatar
  • 101
0 votes
0 answers
31 views

Can the complement of the start sequence occur in a gene?

Put another way, I suppose I'm wondering if the start sequence of a gene always actually starts a gene. If so, I assume it means that the complement of the start sequence in one strand would always ...
creallf's user avatar
  • 101
0 votes
0 answers
30 views

What is the enzyme production rate of a single E. Coli bacterium?

Suppose an E.Coli bacterium intakes a plasmid which encodes for an enzyme, if the promoter strength is known, is is possible to predict the rate at which the enzyme is produced? Is there an equation ...
Vicenzo DeVito's user avatar
0 votes
0 answers
74 views

If RNA transcription is 5' end to 3' end, what is the directionality of the promoter for?

I am confused. If RNA transcription always grows from the 5' end to the 3' end, what does the direction of the promoter do for the mRNA transcribed? From what i understand the RNA polymerase always ...
green onion's user avatar
0 votes
0 answers
55 views

Do transcription factors need to undergo extensive post-translational modification?

Some proteins need additional processing to become fully functional, for example NiFe-hydrogenases need to be cleaved by endopeptidases for some of their subunits to be active. Is it known whether ...
JordanJordan's user avatar
0 votes
0 answers
31 views

Does the two-state gene expression model apply to constitutive genes?

Wikipedia defines constitutive genes as a gene that is transcribed continually as opposed to a facultative gene, which is only transcribed when needed. I don't have a strong theoretical background ...
pew31's user avatar
  • 9
0 votes
0 answers
35 views

Why majority of synthesized mRNAs are halted at Nuclear Pore Complex?

I was reading about mRNA export process. I came to know that only 36% of synthesized mRNA transport events at Nuclear Pore Complexes(NPC) of the nuclear membrane get successful in crossing to the ...
dexterdev's user avatar
  • 1,133
0 votes
0 answers
439 views

What are the causes of abortive initiation?

I was reading more about DNA transcription, and it mentioned abortive initiation. The article gave no explanation as to why the phenomenon occurs. The only explanation I can think of is that it is ...
Hawkeye's user avatar
  • 596
0 votes
0 answers
203 views

What is regulatory promoter?

I came across this term in University question paper. What is the difference between core promoter and regulatory promoter? I have googled (Web, Scholar and Books) it but couldn't find any ...
Tyto alba's user avatar
  • 8,782
0 votes
0 answers
1k views

Does prokaryotic transcription have activators / enhancer regions involved?

I am designing a biosensor, and I need to know whether prokaryotic transcription involves or can involve (if a gene needs to be regulated) enhancer regions. Also, where are enhancer regions located (...
A. Radek Martinez's user avatar