All Questions
2 questions
4
votes
1
answer
281
views
How does the DNA cross through bacterial cell wall during electroporation?
There exists a lot of literature on electroporation of Gram-positive and negative bacteria. Most of it gives an explanation that electroporation works by creating transient pores in cell membranes of ...
- 155
3
votes
1
answer
649
views
Plasmid Design and Integration events [Single vs Double cross over]
When linearising a vector by restriction digest within the middle of a homologous region can a single cross over integration event only occur if the plasmid is re-ligated within the cell after ...
- 31