All Questions
Tagged with transformation ecoli
3 questions
3
votes
1
answer
2k
views
Problem with bacterial transformation with electroporation
I have a problem with a bacterial transformation of a yeast gene that I can not solve.
I isolated yeast DNA and did a PCR to get my product. I am using pCGCUm vector with a GFP construct. I digest ...
- 338
1
vote
1
answer
117
views
How can I improve efficiency of Ecoli transformation?
I am an intern in biology institute. I have a 17.3kbp plasmid need to transform to Ecoli. But I have tried many time but have no or very few colony on LB plate.
I know large plasmid have less ...
- 11
0
votes
1
answer
33
views
I accidentally plated DH5a E. coli and left them in the 4℃ for 12 hours, if I put them into the 37℃ incubator will they start growing?
I started ligation of my constructs late last night and was super tired, since I used all of my ligation mixture I would rather not have to re-ligate.
I did my transformation procedure as follows:
...
- 19