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Questions tagged [vector]

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Do expression vectors themselves contain ATG? [closed]

Do expression vectors themselves contain ATG codon?
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Yeast integrating vectors: where to cut?

I understand that yeast integrating plasmids (YIps) must be linearized in order to promote homology-directed recombination. However I don't quite understand where. In the end, the external regions of ...
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Are there vectors available without any promoter?

Looking for a vector with the following characteristics: 1. Without any promoter 2. Selectable marker: Puromycin 3. Blunt end restriction sites 4. Transfection host: Human or Mouse cell lines.
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How do I reverse my insert within a plasmid?

I have a plasmid with: ...T7pro. -- RBS -- XBa1 cut site -- ProDpro. -- RBS -- AmilCP -- T -- Spe1 cut site -- T... How do I design primers to reverse the portion in bold, such that the T7 and ProD ...
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What are the advantages using SUMO vector for expression? [closed]

My question is : What are the advantages using SUMO vector for expression?
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Debug Fresno; why are the released mosquitos said to be sterile?

I saw in the news that Verily Life Sciences will participate in an experimental release of sterile male aedes aegypti mosquitos in Fresno, California. Aedes aegypti is considered an invasive species ...