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Alkaline Phosphatase and Ligase Protocol for Cloning

In the image the circular molecule is a restricted vector and the linear red molecule is a DNA insert. I found this protocol in my lessons notes, but I don't understand how it is possible that ligase ...
Mirko's user avatar
  • 221
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Cloning using pET28a and Protein Expression in DH5alpha and BL21

Can someone please direct me to an e-resource or a book that will help a newbie like me learn in depth about Cloning using pET28a and Protein Expression in DH5alpha and BL21. Though I have done ...
Carica Rubus's user avatar
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1 answer
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Unusual terminology of vector used

What is the significance of a+ in the name pET-28 a(+)? Is there any a- strain and differences between a,a+,a-?
Arnab Ray's user avatar